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Liver Arginase Recombinant Rabbit Monoclonal Antibody [PSH09-78]

Usd: 385

Specification

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Catalog# HA723143

Liver Arginase Recombinant Rabbit Monoclonal Antibody [PSH09-78]

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Liver Arginase Recombinant Rabbit Monoclonal Antibody [PSH09-78]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human Liver Arginase aa 1-322/322

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 35 kDa

Positive Control

Mouse liver tissue lysate, Rat liver tissue lysate, human liver tissue, mouse liver tissue, rat liver tissue, human liver tissue lysates.

Conjugation

unconjugated

Clone Number

PSH09-78

Reactivity Data

WB IHC-P IF-Tissue
Human
Tested Tested
Tested Tested
Tested Tested
Mouse
Tested Tested
Tested Tested
Tested Tested
Rat
Tested Tested
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:10,000

  • IHC-P

  • 1:2,000-1:15,000

  • IF-Tissue

  • 1:500-1:3,000

Target

Function

Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.Defects in ARG1 are the cause of argininemia (ARGIN); also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia.

Background References

1. Crombez EA, Cederbaum SD. Hyperargininemia due to liver arginase deficiency. Mol Genet Metab. 2005 Mar;84(3):243-51. doi: 10.1016/j.ymgme.2004.11.004. Epub 2004 Dec 19. PMID: 15694174.

2. Maharem TM, Zahran WE, Hassan RE, Abdel Fattah MM. Unique properties of arginase purified from camel liver cytosol. Int J Biol Macromol. 2018 Mar;108:88-97. doi: 10.1016/j.ijbiomac.2017.11.141. Epub 2017 Nov 24. PMID: 29180053.

Subcellular Location

Cytoplasm , Cytoplasmic granule

UNIPROT

SwissProt: P05089 Human

SwissProt: Q61176 Mouse

SwissProt: P07824 Rat

Synonyms

A I antibody

Al antibody

ARG 1 antibody

arg1 antibody

ARGI1_HUMAN antibody

Arginase 1 antibody

Arginase liver antibody

Arginase type I antibody

Arginase, liver antibody

Arginase-1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Liver Arginase on different lysates with Rabbit anti-Liver Arginase antibody (<a href="/products/HA723143" style="font-weight: bold;text-decoration: underline;">HA723143</a>) at 1/10,000 dilution.<br /><br />Lane 1: Mouse liver tissue lysate (40 µg/Lane)<br />Lane 2: Mouse skeletal muscle tissue lysate (negative) (40 µg/Lane)<br />Lane 3: Rat liver tissue lysate (40 µg/Lane)<br />Lane 4: Rat skeletal muscle tissue lysate (negative) (40 µg/Lane)<br /><br />Predicted band size: 35 kDa<br />Observed band size: 37 kDa<br />Exposure time: 2 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723143" style="font-weight: bold;text-decoration: underline;">HA723143</a>) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of Liver Arginase on different lysates with Rabbit anti-Liver Arginase antibody (HA723143) at 1/10,000 dilution.

    Lane 1: Mouse liver tissue lysate (40 µg/Lane)
    Lane 2: Mouse skeletal muscle tissue lysate (negative) (40 µg/Lane)
    Lane 3: Rat liver tissue lysate (40 µg/Lane)
    Lane 4: Rat skeletal muscle tissue lysate (negative) (40 µg/Lane)

    Predicted band size: 35 kDa
    Observed band size: 37 kDa
    Exposure time: 2 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723143) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Liver Arginase antibody (<a href="/products/HA723143" style="font-weight: bold;text-decoration: underline;">HA723143</a>) at 1/15,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723143" style="font-weight: bold;text-decoration: underline;">HA723143</a>) at 1/15,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Liver Arginase antibody (HA723143) at 1/15,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723143) at 1/15,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue (negative) with Rabbit anti-Liver Arginase antibody (<a href="/products/HA723143" style="font-weight: bold;text-decoration: underline;">HA723143</a>) at 1/15,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723143" style="font-weight: bold;text-decoration: underline;">HA723143</a>) at 1/15,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue (negative) with Rabbit anti-Liver Arginase antibody (HA723143) at 1/15,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723143) at 1/15,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Liver Arginase antibody (<a href="/products/HA723143" style="font-weight: bold;text-decoration: underline;">HA723143</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723143" style="font-weight: bold;text-decoration: underline;">HA723143</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Liver Arginase antibody (HA723143) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723143) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Liver Arginase antibody (<a href="/products/HA723143" style="font-weight: bold;text-decoration: underline;">HA723143</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723143" style="font-weight: bold;text-decoration: underline;">HA723143</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Liver Arginase antibody (HA723143) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723143) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Western blot analysis of Liver Arginase on human liver tissue lysates with Rabbit anti-Liver Arginase antibody (<a href="/products/HA723143" style="font-weight: bold;text-decoration: underline;">HA723143</a>) at 1/10,000 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 35 kDa<br />Observed band size: 35 kDa<br /><br />Exposure time: 16 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723143" style="font-weight: bold;text-decoration: underline;">HA723143</a>) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Liver Arginase on human liver tissue lysates with Rabbit anti-Liver Arginase antibody (HA723143) at 1/10,000 dilution.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 35 kDa
    Observed band size: 35 kDa

    Exposure time: 16 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723143) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation