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Glucose Transporter GLUT4 Recombinant Rabbit Monoclonal Antibody [PSH10-06]

Usd: 385

Specification

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Catalog# HA723175

Glucose Transporter GLUT4 Recombinant Rabbit Monoclonal Antibody [PSH10-06]

Application

  • WB

  • IHC-Fr

Reactivity

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Glucose Transporter GLUT4 Recombinant Rabbit Monoclonal Antibody [PSH10-06]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human GLUT4 aa 447-509.

Species Reactivity

Mouse, Rat

Validated Applications

WB, IHC-Fr

Molecular Weight

Predicted band size: 55 kDa

Positive Control

Mouse heart tissue, rat skeletal muscle tissue, Mouse heart tissue lysate, Mouse skeletal muscle tissue lysate, Rat skeletal muscle tissue lysate.

Conjugation

unconjugated

Clone Number

PSH10-06

Reactivity Data

WB IHC-Fr
Mouse
Tested Tested
Tested Tested
Rat
Tested Tested
Tested Tested
Human

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-Fr

  • 1:500

Target

Function

Glucose transporter type 4 (GLUT4), also known as solute carrier family 2, facilitated glucose transporter member 4, is a protein encoded, in humans, by the SLC2A4 gene. GLUT4 is the insulin-regulated glucose transporter found primarily in adipose tissues and striated muscle (skeletal and cardiac). At the cell surface, GLUT4 permits the facilitated diffusion of circulating glucose down its concentration gradient into muscle and fat cells. Once within cells, glucose is rapidly phosphorylated by glucokinase in the liver and hexokinase in other tissues to form glucose-6-phosphate, which then enters glycolysis or is polymerized into glycogen. Glucose-6-phosphate cannot diffuse back out of cells, which also serves to maintain the concentration gradient for glucose to passively enter cells.

Background References

1. van Gerwen J et al. Insulin signalling and GLUT4 trafficking in insulin resistance. Biochem Soc Trans. 2023 Jun

2. Yuan Y et al. Cryo-EM structure of human glucose transporter GLUT4. Nat Commun. 2022 May

Subcellular Location

Cell membrane, Endomembrane system, Cytoplasm, perinuclear region.

UNIPROT

SwissProt: P14142 Mouse

SwissProt: P19357 Rat

Synonyms

insulin-responsive antibody

Glucose transporter GLUT 4 antibody

Glucose transporter type 4 antibody

Glucose transporter type 4 insulin responsive antibody

GLUT 4 antibody

GLUT-4 antibody

GLUT4 antibody

GTR4_HUMAN antibody

Insulin responsive glucose transporter type 4 antibody

kug antibody

Expand

Images

  • Immunofluorescence analysis of frozen mouse heart tissue with Rabbit anti-Glucose Transporter GLUT4 antibody (<a href="/products/HA723175" style="font-weight: bold;text-decoration: underline;">HA723175</a>) at 1/500 dilution.<br /><br /><span style="font-weight: bold;">The section was not undergone antigen retrieval.</span> The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA723175" style="font-weight: bold;text-decoration: underline;">HA723175</a>, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of frozen mouse heart tissue with Rabbit anti-Glucose Transporter GLUT4 antibody (HA723175) at 1/500 dilution.

    The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA723175, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

  • Immunofluorescence analysis of frozen rat skeletal muscle tissue with Rabbit anti-Glucose Transporter GLUT4 antibody (<a href="/products/HA723175" style="font-weight: bold;text-decoration: underline;">HA723175</a>) at 1/500 dilution.<br /><br /><span style="font-weight: bold;">The section was not undergone antigen retrieval.</span> The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA723175" style="font-weight: bold;text-decoration: underline;">HA723175</a>, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of frozen rat skeletal muscle tissue with Rabbit anti-Glucose Transporter GLUT4 antibody (HA723175) at 1/500 dilution.

    The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA723175, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Glucose Transporter GLUT4 on different lysates with Rabbit anti-Glucose Transporter GLUT4 antibody (<a href="/products/HA723175" style="font-weight: bold;text-decoration: underline;">HA723175</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse heart tissue lysate (no heat) (30 µg/Lane)<br />Lane 2: Mouse skeletal muscle tissue lysate (no heat) (30 µg/Lane)<br />Lane 3: Mouse kidney tissue lysate (no heat) (negative) (30 µg/Lane)<br />Lane 4: Rat kidney tissue lysate (negative) (30 µg/Lane)<br />Lane 5: Rat lung tissue lysate (no heat) (negative) (30 µg/Lane)<br />Lane 6: Rat skeletal muscle tissue lysate (no heat) (30 µg/Lane)<br /><br />Notice: no heat means the lysate is not boiled.<br />Predicted band size: 55 kDa<br />Observed band size: 50 kDa<br />Exposure time: 20 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723175" style="font-weight: bold;text-decoration: underline;">HA723175</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of Glucose Transporter GLUT4 on different lysates with Rabbit anti-Glucose Transporter GLUT4 antibody (HA723175) at 1/5,000 dilution.

    Lane 1: Mouse heart tissue lysate (no heat) (30 µg/Lane)
    Lane 2: Mouse skeletal muscle tissue lysate (no heat) (30 µg/Lane)
    Lane 3: Mouse kidney tissue lysate (no heat) (negative) (30 µg/Lane)
    Lane 4: Rat kidney tissue lysate (negative) (30 µg/Lane)
    Lane 5: Rat lung tissue lysate (no heat) (negative) (30 µg/Lane)
    Lane 6: Rat skeletal muscle tissue lysate (no heat) (30 µg/Lane)

    Notice: no heat means the lysate is not boiled.
    Predicted band size: 55 kDa
    Observed band size: 50 kDa
    Exposure time: 20 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723175) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation