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Integrin beta 5 Recombinant Rabbit Monoclonal Antibody [PSH10-13]

Usd: 385

Specification

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Catalog# HA723182

Integrin beta 5 Recombinant Rabbit Monoclonal Antibody [PSH10-13]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Rat

  • Green monkey

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

Integrin beta 5 Recombinant Rabbit Monoclonal Antibody [PSH10-13]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human Integrin beta 5 aa 1-719.

Species Reactivity

Human, Rat, Green monkey

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 88 kDa

Positive Control

A549 cell lysate, HT-29 cell lysate, NCI-H1299 cell lysate, PANC-1 cell lysate, COS-1 cell lysate, C6 cell lysate, Rat lung tissue lysate, Rat kidney tissue lysate.

Conjugation

unconjugated

Clone Number

PSH10-13

Reactivity Data

WB IHC-P
Human
Tested Tested
Not Recommended
Rat
Tested Tested
Tested Tested
Green Monkey
Tested Tested
Mouse
Not Recommended
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:200-1:1,000

Target

Function

Integrin beta-5 is a protein that in humans is encoded by the ITGB5 gene. Integrin, beta 5 has been shown to interact with PTK2, Annexin A5 and PAK4. ITGB5 encodes a subunit of integrin that can interact with several alpha chains to form a variety of integrin heterodimers. It also plays a potential role in intercellular communication during tumor progression and metastasis.

Background References

1. Lei T et al. ITGB5 facilitates gastric cancer metastasis by promoting TGFBR2 endosomal recycling. Cancer Lett. 2024 Jun

2. Wen X et al. ITGB5 promotes innate radiation resistance in pancreatic adenocarcinoma by promoting DNA damage repair and the MEK/ERK signaling pathway. Front Oncol. 2022 Sep

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P18084 Human

Entrez Gene: 257645 Rat

Synonyms

FLJ26658 antibody

Integrin beta-5 antibody

Integrin subunit beta 5 antibody

integrin, beta 5 antibody

ITB5_HUMAN antibody

Itgb5 antibody

Testis secretory sperm-binding protein Li 217p antibody

Images

  • Western blot analysis of Integrin beta 5 on different lysates with Rabbit anti-Integrin beta 5 antibody (<a href="/products/HA723182" style="font-weight: bold;text-decoration: underline;">HA723182</a>) at 1/2,000 dilution.<br /><br />Lane 1: A549 cell lysate<br />Lane 2: HT-29 cell lysate<br />Lane 3: NCI-H1299 cell lysate<br />Lane 4: PANC-1 cell lysate<br />Lane 5: COS-1 cell lysate<br />Lane 6: C6 cell lysate<br />Lane 7: Rat lung tissue lysate<br />Lane 8: Rat kidney tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 88 kDa<br />Observed band size: 88 kDa<br /><br />Exposure time: 1 minute 20 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723182" style="font-weight: bold;text-decoration: underline;">HA723182</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Integrin beta 5 on different lysates with Rabbit anti-Integrin beta 5 antibody (HA723182) at 1/2,000 dilution.

    Lane 1: A549 cell lysate
    Lane 2: HT-29 cell lysate
    Lane 3: NCI-H1299 cell lysate
    Lane 4: PANC-1 cell lysate
    Lane 5: COS-1 cell lysate
    Lane 6: C6 cell lysate
    Lane 7: Rat lung tissue lysate
    Lane 8: Rat kidney tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 88 kDa
    Observed band size: 88 kDa

    Exposure time: 1 minute 20 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723182) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Integrin beta 5 antibody (<a href="/products/HA723182" style="font-weight: bold;text-decoration: underline;">HA723182</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723182" style="font-weight: bold;text-decoration: underline;">HA723182</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Integrin beta 5 antibody (HA723182) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723182) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-Integrin beta 5 antibody (<a href="/products/HA723182" style="font-weight: bold;text-decoration: underline;">HA723182</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723182" style="font-weight: bold;text-decoration: underline;">HA723182</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-Integrin beta 5 antibody (HA723182) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723182) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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