Phospho-PPP1CA (T320) Recombinant Rabbit Monoclonal Antibody [PSH10-27]
Catalog# HA723194
Phospho-PPP1CA (T320) Recombinant Rabbit Monoclonal Antibody [PSH10-27]
-
WB
-
IHC-P
-
IP
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
Phospho-PPP1CA (T320) Recombinant Rabbit Monoclonal Antibody [PSH10-27]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Thr320 of Human PPP1CA.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IP
Target Molecular Weight
Predicted band size: 38 kDa
Positive Control
HeLa treated with 100nM Calyculin A for 30 minutes cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, PC-12 treated with 100ng/mL Nocodazole for 18 hours cell lysate, human breast cancer tissue, human testis tissue, rat testis tissue.
Conjugation
unconjugated
Clone Number
PSH10-27
Reactivity Data
| WB | IHC-P | IP | |
|---|---|---|---|
| Human |
|
|
|
| Mouse |
|
|
|
| Rat |
|
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000
-
IHC-P
-
1:20,000
-
IP
-
1-2μg/sample
Target
Function
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit is an enzyme that in humans is encoded by the PPP1CA gene. The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Background References
1. Zhang L et al. Arginine methylation of PPP1CA by CARM1 regulates glucose metabolism and affects osteogenic differentiation and osteoclastic differentiation. Clin Transl Med. 2023 Sep
2. Liang H et al. Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration. Cell Death Differ. 2023 Sep
Subcellular Location
Cytoplasm, Nucleus, nucleoplasm, nucleolus.
Synonyms
Alpha isoform serine threonine protein phosphatase PP1alpha 1 catalytic subunit antibody
Catalytic subunit antibody
EC 3.1.3.16 antibody
MGC15877 antibody
MGC1674 antibody
PP 1A antibody
PP-1A antibody
PP1A antibody
PP1A_HUMAN antibody
PP1alpha antibody
ExpandImages
-
☑ Cell treatment (CT)
Western blot analysis of Phospho-PPP1CA (T320) on different lysates with Rabbit anti-Phospho-PPP1CA (T320) antibody (HA723194) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: PC-12 cell lysate
Lane 5: PC-12 treated with 100ng/mL Nocodazole for 18 hours cell lysate
Lane 6: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 38 kDa
Observed band size: 35 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723194) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Phospho-PPP1CA (T320) antibody (HA723194) at 1/20,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723194) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Phospho-PPP1CA (T320) antibody (HA723194) at 1/20,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723194) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-Phospho-PPP1CA (T320) antibody (HA723194) at 1/20,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723194) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Cell treatment (CT)
Phospho-PPP1CA (T320) was immunoprecipitated from 0.2 mg HeLa treated with 50mM Calyculin A for 3 hours cell lysate with HA723194 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723194 at 1/2,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa treated with 50mM Calyculin A for 3 hours cell lysate (input)
Lane 2: HA723194 IP in HeLa treated with 50mM Calyculin A for 3 hours cell lysate
Lane 3: Rabbit IgG instead of HA723194 in HeLa treated with 50mM Calyculin A for 3 hours cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 1 minute 23 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
PPP1CA Rabbit Polyclonal Antibody
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
