Tau Recombinant Rabbit Monoclonal Antibody [PSH10-42] - BSA and Azide free (Detector)
Catalog# HA723209
Tau Recombinant Rabbit Monoclonal Antibody [PSH10-42] - BSA and Azide free (Detector)
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ELISA(Det)
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Human
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Mouse
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Rat
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5.92
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unconjugated
Overview
Product Name
Tau Recombinant Rabbit Monoclonal Antibody [PSH10-42] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human Tau aa 57-68.
Species Reactivity
Human, Mouse, Rat
Validated Applications
ELISA(Det)
Conjugation
unconjugated
Clone Number
PSH10-42
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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ELISA(Det)
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Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-41] to Human Phospho-Tau (T217) antibody (Capture) (HA723841) and Recombinant Human Phospho-Tau (T217) protein (HA211033) as the standard. The reference range value is 0.195-50 ng/mL.
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ELISA(Det)
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Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-43] to Human Non-Phoshpho-Tau (T217) antibody (Capture) (HA723843) and Recombinant Human Phospho-Tau (T217) protein (HA210937) as the standard. The reference range value is 0.117-30 pg/mL.
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ELISA(Det)
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Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-46] to Brain-derived tau/BD-tau antibody (Capture) (HA723847) and Recombinant Human Tau/Tau441 protein (HA210937) as the standard. The reference range value is 39-10,000 pg/mL.
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ELISA(Det)
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Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-48] to Tau antibody (Capture) (HA723850) and Recombinant Human Tau/Tau441 protein (HA210937) as the standard. The reference range value is 39-5,000 pg/mL.
Target
Function
Tau, also known as MAPT (microtubule-associated protein tau), MAPTL, MTBT1 or TAU, is a 758 amino acid protein that localizes to the cytoplasm, as well as to the cytoskeleton and the cell membrane, and contains four Tau/MAP repeats. Expressed in neuronal tissue and existing as multiple alternatively spliced isoforms, Tau functions to promote microtubule assembly and stability and is thought to be involved in the maintenance of neuronal polarity. Tau may also link microtubules with neural plasma membrane components and, addition to its role in microtubule stability, is also necessary for cytoskeletal plasticity. Tau is highly subject to a variety of post-translational modifications, including phosphorylation on serine and threonine residues, polyubiquitination (and subsequent proteasomal degradation) and glycation of specific Tau isoforms. Defects in the gene encoding Tau are associated with Alzheimers disease, pallido-ponto-nigral degeneration (PPND), corticobasal degeneration (CBD) and progressive supranuclear palsy (PSP).
Background References
1. Wang, HY. et al. 2012. Reducing amyloid-related Alzheimer\'s disease pathogenesis by a small molecule targeting filamin A. J. Neurosci. 32: 9773-9784.
2. Kamnaksh, A. et al. 2012. Neurobehavioral, cellular, and molecular consequences of single and multiple mild blast exposure. Electrophoresis. 33: 3680-3692.
Subcellular Location
c
Synonyms
AI413597 antibody
AW045860 antibody
DDPAC antibody
FLJ31424 antibody
FTDP 17 antibody
G protein beta1/gamma2 subunit interacting factor 1 antibody
MAPT antibody
MAPTL antibody
MGC134287 antibody
MGC138549 antibody
ExpandImages
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Sandwich ELISA analysis of Human Tau (phospho T217) matched pair antibodies
Capture: HA723841, Phospho-Tau (T217) Rabbit mAb [PSH16-41]
Detector: HA723209, Phospho-Tau (T217) Rabbit mAb [PSH10-42]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723841) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Phospho-Tau (T217) protein (HA211033) starting from 50000 pg/ml to 0 pg/ml and detect antibody (HA723209, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
Sandwich ELISA analysis of Human Non-p217 Tau matched pair antibodies
Capture: HA723843, Phospho-Tau (T217) Rabbit mAb [PSH16-43]
Detector: HA723209, Phospho-Tau (T217) Rabbit mAb [PSH10-42]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723843) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Phospho-Tau (T217) protein (HA210937) starting from 30000 pg/ml to 0 pg/ml and detect antibody (HA723209, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
Sandwich ELISA analysis of Human Brain-derived tau/BD-tau matched pair antibodies
Capture: HA723847, Brain-derived tau/BD-tau Rabbit mAb [PSH16-46]
Detector: HA723209, Brain-derived tau/BD-tau Rabbit mAb [PSH10-42]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723847) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Brain-derived tau/BD-tau protein (HA210937) starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA723209, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
Sandwich ELISA analysis of human Tau matched pair antibodies
Capture: HA723850, Tau Rabbit mAb [PSH16-48]
Detector: HA723209, Tau Rabbit mAb [PSH10-42]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723850) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Tau protein (HA210937) starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA723209, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
Sandwich ELISA analysis of Human Tau (phospho T217) matched pair antibodies
Capture: HA723877, Phospho-Tau (T217) Rabbit mAb [PSH16-75]
Detector: HA723209, Phospho-Tau (T217) Rabbit mAb [PSH10-42]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723877) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human Phospho-Tau (T217) protein (HA211033) starting from 15000 pg/ml to 0 pg/ml and detect antibody (HA723209, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"