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VAChT / SLC18A3 Recombinant Rabbit Monoclonal Antibody [PSH10-50]

Usd: 385

Specification

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Catalog# HA723214

VAChT / SLC18A3 Recombinant Rabbit Monoclonal Antibody [PSH10-50]

Application

  • WB

  • IHC-Fr

  • IHC-P

Reactivity

  • Mouse

  • Rat

BSA and Azide free
Predicted reactivity

  • Cynomolgus monkey

  • Pig

Predicted species support after-sales service
Conjugation

  • unconjugated

Overview

Product Name

VAChT / SLC18A3 Recombinant Rabbit Monoclonal Antibody [PSH10-50]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within N terminal Mouse VAChT.

Species Reactivity

Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

Predicted species support after-sales service

Validated Applications

WB, IHC-Fr, IHC-P

Molecular Weight

Predicted band size: 57 kDa

Positive Control

Mouse brain tissue, mouse striatum tissue, rat brain tissue, rat striatum tissue, Mouse brain tissue lysate, Rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

PSH10-50

Reactivity Data

WB IHC-Fr IHC-P
Mouse
Tested Tested
Tested Tested
Tested Tested
Rat
Tested Tested
Tested Tested
Tested Tested
Cynomolgus Monkey
Published
Published
Published
Pig
Published
Published
Published

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-Fr

  • 1:500

  • IHC-P

  • 1:1,000

Target

Function

The Vesicular acetylcholine transporter (VAChT) is a neurotransmitter transporter which is responsible for loading acetylcholine (ACh) into secretory organelles in neurons making acetylcholine available for secretion. It is encoded by Solute carrier family 18, member 3 (SLC18A3) gene, located within the first intron of the choline acetyltransferase gene. VAChT is able to transport ACh into vesicles by relying on an exchange between protons (H+) that were previously pumped into the vesicle diffusing out, thus acting as an antiporter. ACh molecules are then carried into the vesicle by the action of exiting protons. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase.

Background References

1. Zhang Y et al. Structural insights into VAChT neurotransmitter recognition and inhibition. Cell Res. 2024 Sep

2. Pergolizzi S et al. Expression of VAChT and 5-HT in Ulcerative colitis dendritic cells. Acta Histochem. 2021 May

Subcellular Location

Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane.

UNIPROT

SwissProt: O35304 Mouse

SwissProt: Q62666 Rat

Synonyms

AChR antibody

MGC12716 antibody

rVAT antibody

Slc18a3 antibody

Solute carrier family 18 (vesicular acetylcholine) member 3 antibody

Solute carrier family 18 (vesicular monoamine) member 3 antibody

Solute carrier family 18 member 3 antibody

VAChT antibody

VACHT_HUMAN antibody

Vesicular acetylcholine transporter antibody

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebral cortex<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebral cortex

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: Cerebral cortex<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Rat

    Site: Cerebral cortex

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-VAChT / SLC18A3 antibody (<a href="/products/HA723214" style="font-weight: bold;text-decoration: underline;">HA723214</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723214" style="font-weight: bold;text-decoration: underline;">HA723214</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-VAChT / SLC18A3 antibody (HA723214) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723214) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Rabbit anti-VAChT / SLC18A3 antibody (<a href="/products/HA723214" style="font-weight: bold;text-decoration: underline;">HA723214</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723214" style="font-weight: bold;text-decoration: underline;">HA723214</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Rabbit anti-VAChT / SLC18A3 antibody (HA723214) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723214) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-VAChT / SLC18A3 antibody (<a href="/products/HA723214" style="font-weight: bold;text-decoration: underline;">HA723214</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723214" style="font-weight: bold;text-decoration: underline;">HA723214</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-VAChT / SLC18A3 antibody (HA723214) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723214) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat striatum tissue with Rabbit anti-VAChT / SLC18A3 antibody (<a href="/products/HA723214" style="font-weight: bold;text-decoration: underline;">HA723214</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723214" style="font-weight: bold;text-decoration: underline;">HA723214</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat striatum tissue with Rabbit anti-VAChT / SLC18A3 antibody (HA723214) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723214) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of VAChT / SLC18A3 on different lysates with Rabbit anti-VAChT / SLC18A3 antibody (<a href="/products/HA723214" style="font-weight: bold;text-decoration: underline;">HA723214</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate<br />Lane 2: Mouse kidney tissue lysate (low expression)<br />Lane 3: Rat brain tissue lysate<br />Lane 4: Rat kidney tissue lysate (low expression)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 57 kDa<br />Observed band size: 70 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723214" style="font-weight: bold;text-decoration: underline;">HA723214</a>) at 1/1,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of VAChT / SLC18A3 on different lysates with Rabbit anti-VAChT / SLC18A3 antibody (HA723214) at 1/1,000 dilution.

    Lane 1: Mouse brain tissue lysate
    Lane 2: Mouse kidney tissue lysate (low expression)
    Lane 3: Rat brain tissue lysate
    Lane 4: Rat kidney tissue lysate (low expression)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 57 kDa
    Observed band size: 70 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723214) at 1/1,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"