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VGLUT2 Recombinant Rabbit Monoclonal Antibody [PSH10-54]

Usd: 385

Specification

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Catalog# HA723218

VGLUT2 Recombinant Rabbit Monoclonal Antibody [PSH10-54]

Application

  • WB

  • IHC-Fr

  • IHC-P

  • IF-Tissue

Reactivity

  • Mouse

  • Rat

BSA and Azide free
Predicted reactivity

  • Cynomolgus monkey

  • Pig

Conjugation

  • unconjugated

Overview

Product Name

VGLUT2 Recombinant Rabbit Monoclonal Antibody [PSH10-54]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Species Reactivity

Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

Predicted species support after-sales service

Validated Applications

WB, IHC-Fr, IHC-P, IF-Tissue

Target Molecular Weight

Predicted band size: 65 kDa

Positive Control

Mouse brain tissue, mouse hippocampus tissue, mouse cerebellum tissue, rat brain tissue, rat hippocampus tissue, rat cerebellum tissue, Mouse brain tissue lysate, Rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

PSH10-54

Reactivity Data

WB IHC-Fr IHC-P IF-Tissue
Mouse
Tested Tested
Tested Tested
Tested Tested
Rat
Tested Tested
Tested Tested
Tested Tested
Cynomolgus Monkey
Published
Published
Published
Published
Pig
Published
Published
Published
Published

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-Fr

  • 1:200-1:500

  • IHC-P

  • 1:2,000

  • IF-Tissue

  • 1:500

Target

Function

Predicted to enable L-glutamate transmembrane transporter activity and neurotransmitter transmembrane transporter activity. Involved in neurotransmitter loading into synaptic vesicle. Predicted to be located in synaptic vesicle. Predicted to be active in excitatory synapse. Predicted to be integral component of synaptic vesicle membrane.

Background References

1. Buck SA et al. Roles of VGLUT2 and Dopamine/Glutamate Co-Transmission in Selective Vulnerability to Dopamine Neurodegeneration. ACS Chem Neurosci. 2022 Jan

2. Zhang Y et al. VGluT2 neuron subtypes in the paraventricular thalamic nucleus regulate depression in paraquat-induced Parkinson\'s disease. J Hazard Mater. 2024 Jul

Subcellular Location

Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Synapse, synaptosome, Cell membrane.

UNIPROT

SwissProt: Q8BLE7 Mouse

SwissProt: Q9JI12 Rat

Synonyms

Differentiation associated BNPI antibody

Differentiation associated Na dependent inorganic phosphate cotransporter antibody

Differentiation associated Na(+) dependent inorganic phosphate cotransporter antibody

Differentiation-associated BNPI antibody

Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter antibody

DNPI antibody

SLC17A6 antibody

Sodium dependent inorganic phosphate cotransporter antibody

Solute carrier family 17 (Sodium dependent inorganic phosphate cotransporter) member 6 antibody

Solute carrier family 17 member 6 antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Hippocampus<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500 (VGLUT2, <a href="/products/HA723218" style="font-weight: bold;text-decoration: underline;">HA723218</a>, red); 1/500 (VGAT, <a href="/products/HA601384" style="font-weight: bold;text-decoration: underline;">HA601384</a>, green)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Hippocampus

    Sample: Frozen section

    Antibody concentration: 1/500 (VGLUT2, HA723218, red); 1/500 (VGAT, HA601384, green)

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Hippocampus<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1: 500 (VGLUT2, <a href="/products/HA723218" style="font-weight: bold;text-decoration: underline;">HA723218</a>, red), 1:500 (VGluT1, <a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>, green)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Hippocampus

    Sample: Frozen section

    Antibody concentration: 1: 500 (VGLUT2, HA723218, red), 1:500 (VGluT1, HA601392, green)

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebral cortex<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1: 500 (VGLUT2, <a href="/products/HA723218" style="font-weight: bold;text-decoration: underline;">HA723218</a>, red), 1:500 (VGluT1, <a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>, green)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebral cortex

    Sample: Frozen section

    Antibody concentration: 1: 500 (VGLUT2, HA723218, red), 1:500 (VGluT1, HA601392, green)

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: Hippocampus<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1: 500 (VGLUT2, <a href="/products/HA723218" style="font-weight: bold;text-decoration: underline;">HA723218</a>, red), 1:500 (VGluT1, <a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>, green)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Rat

    Site: Hippocampus

    Sample: Frozen section

    Antibody concentration: 1: 500 (VGLUT2, HA723218, red), 1:500 (VGluT1, HA601392, green)

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: Cerebral cortex<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1: 500 (VGLUT2, <a href="/products/HA723218" style="font-weight: bold;text-decoration: underline;">HA723218</a>, red), 1:500 (VGluT1, <a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>, green)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Rat

    Site: Cerebral cortex

    Sample: Frozen section

    Antibody concentration: 1: 500 (VGLUT2, HA723218, red), 1:500 (VGluT1, HA601392, green)

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebellum<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1: 500 <br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1: 500

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: Cerebellum<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1: 500 <br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Rat

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1: 500

    Antigen retrieval: Not required

  • Application: Immunofluorescence (IHC-Fr)<br /><br />Species: Mouse<br />Tissue: Spinal cord<br />Sample: Frozen section<br /><br />Antigen retrieval: Not required<br /><br />Wash buffer: 1× PBS<br />Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.<br />Primary antibody: VGLUT2: 1/500 (<a href="/products/HA723218" style="font-weight: bold;text-decoration: underline;">HA723218</a>, rabbit, Green), NeuN: 1/1,000 (<a href="/products/HA601482" style="font-weight: bold;text-decoration: underline;">HA601482</a>, mouse, Red), overnight at 4℃.<br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), Goat anti-Mouse IgG (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IHC-Fr)

    Species: Mouse
    Tissue: Spinal cord
    Sample: Frozen section

    Antigen retrieval: Not required

    Wash buffer: 1× PBS
    Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
    Primary antibody: VGLUT2: 1/500 (HA723218, rabbit, Green), NeuN: 1/1,000 (HA601482, mouse, Red), overnight at 4℃.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), Goat anti-Mouse IgG (iFluor™ 594, HA1126), 1.5 hours at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-VGLUT2 antibody (<a href="/products/HA723218" style="font-weight: bold;text-decoration: underline;">HA723218</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723218" style="font-weight: bold;text-decoration: underline;">HA723218</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-VGLUT2 antibody (HA723218) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723218) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-VGLUT2 antibody (<a href="/products/HA723218" style="font-weight: bold;text-decoration: underline;">HA723218</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723218" style="font-weight: bold;text-decoration: underline;">HA723218</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-VGLUT2 antibody (HA723218) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723218) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-VGLUT2 antibody (<a href="/products/HA723218" style="font-weight: bold;text-decoration: underline;">HA723218</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723218" style="font-weight: bold;text-decoration: underline;">HA723218</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-VGLUT2 antibody (HA723218) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723218) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-VGLUT2 antibody (<a href="/products/HA723218" style="font-weight: bold;text-decoration: underline;">HA723218</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723218" style="font-weight: bold;text-decoration: underline;">HA723218</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-VGLUT2 antibody (HA723218) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723218) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-VGLUT2 antibody (<a href="/products/HA723218" style="font-weight: bold;text-decoration: underline;">HA723218</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723218" style="font-weight: bold;text-decoration: underline;">HA723218</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-VGLUT2 antibody (HA723218) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723218) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-VGLUT2 antibody (<a href="/products/HA723218" style="font-weight: bold;text-decoration: underline;">HA723218</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723218" style="font-weight: bold;text-decoration: underline;">HA723218</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-VGLUT2 antibody (HA723218) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723218) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of VGLUT2 on different lysates with Rabbit anti-VGLUT2 antibody (<a href="/products/HA723218" style="font-weight: bold;text-decoration: underline;">HA723218</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate (20 µg/Lane)<br />Lane 2: Mouse lung tissue lysate (negative) (20 µg/Lane)<br />Lane 3: Rat brain tissue lysate (20 µg/Lane)<br />Lane 4: Rat lung tissue lysate (negative) (20 µg/Lane)<br /><br />Predicted band size: 65 kDa<br />Observed band size: 65 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723218" style="font-weight: bold;text-decoration: underline;">HA723218</a>) at 1/2,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of VGLUT2 on different lysates with Rabbit anti-VGLUT2 antibody (HA723218) at 1/2,000 dilution.

    Lane 1: Mouse brain tissue lysate (20 µg/Lane)
    Lane 2: Mouse lung tissue lysate (negative) (20 µg/Lane)
    Lane 3: Rat brain tissue lysate (20 µg/Lane)
    Lane 4: Rat lung tissue lysate (negative) (20 µg/Lane)

    Predicted band size: 65 kDa
    Observed band size: 65 kDa

    Exposure time: 20 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723218) at 1/2,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Application: IF-tissue<br /><br />Species: Mouse<br /><br />Site: Cerebellum<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/500 (VGLUT2, <a href="/products/HA723218" style="font-weight: bold;text-decoration: underline;">HA723218</a>, Rabbit, red); 1/500 (VGLUT1, <a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>, Rat, green)

    Application: IF-tissue

    Species: Mouse

    Site: Cerebellum

    Sample: Paraffin-embedded section

    Antibody concentration: 1/500 (VGLUT2, HA723218, Rabbit, red); 1/500 (VGLUT1, HA601392, Rat, green)

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