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SHANK2 Recombinant Rabbit Monoclonal Antibody [PSH10-61]

Usd: 385

Specification

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Catalog# HA723226

SHANK2 Recombinant Rabbit Monoclonal Antibody [PSH10-61]

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Predicted reactivity

  • Cynomolgus monkey

Predicted species support after-sales service
Conjugation

  • unconjugated

Overview

Product Name

SHANK2 Recombinant Rabbit Monoclonal Antibody [PSH10-61]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human SHANK2 aa 901-1,230.

Species Reactivity

Human, Mouse, Rat (Predicted: Cynomolgus monkey)

Predicted species support after-sales service

Validated Applications

WB, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 201 kDa

Positive Control

Mouse brain tissue lysate, Rat brain tissue lysate, mouse brain tissue, mouse hippocampus tissue, mouse cerebellum tissue, mouse liver tissue.

Conjugation

unconjugated

Clone Number

PSH10-61

Reactivity Data

WB IHC-P IF-Tissue
Human
Tested Tested
Not Recommended
Mouse
Tested Tested
Tested Tested
Tested Tested
Rat
Tested Tested
Not Recommended
Cynomolgus Monkey
Published
Published
Published

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:50-1:100

  • IF-Tissue

  • 1:200

Target

Function

SH3 and multiple ankyrin repeat domains protein 2 is a protein that in humans is encoded by the SHANK2 gene. Two alternative splice variants, encoding distinct isoforms, are reported. Additional splice variants exist but their full-length nature has not been determined. This gene encodes a protein that is a member of the Shank family of synaptic proteins that may function as molecular scaffolds in the postsynaptic density (PSD). Shank proteins contain multiple domains for protein-protein interaction, including ankyrin repeats, an SH3 domain, a PSD-95/Dlg/ZO-1 domain, a sterile alpha motif domain, and a proline-rich region. This particular family member contains a PDZ domain, a consensus sequence for cortactin SH3 domain-binding peptides and a sterile alpha motif. The alternative splicing demonstrated in Shank genes has been suggested as a mechanism for regulating the molecular structure of Shank and the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain.

Background References

1. Caumes R et al. Phenotypic spectrum of SHANK2-related neurodevelopmental disorder. Eur J Med Genet. 2020 Dec

2. Olde Heuvel F et al. Shank2 identifies a subset of glycinergic neurons involved in altered nociception in an autism model. Mol Autism. 2023 Jun

Subcellular Location

Apical cell membrane, Cytoplasm, Synapse, Postsynaptic density, Cell projection, growth cone, Cell projection, dendritic spine.

UNIPROT

SwissProt: Q9UPX8 Human

SwissProt: Q80Z38 Mouse

SwissProt: Q9QX74 Rat

Synonyms

AUTS17 antibody

Cortactin binding protein 1 antibody

Cortactin SH3 domain-binding protein antibody

Cortactin-binding protein 1 antibody

CortBP1 antibody

CTTNBP1 antibody

GKAP/SAPAP interacting protein antibody

GKAP/SAPAP-interacting protein antibody

KIAA1022 antibody

Proline rich synapse associated protein 1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of SHANK2 on different lysates with Rabbit anti-SHANK2 antibody (<a href="/products/HA723226" style="font-weight: bold;text-decoration: underline;">HA723226</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 2: Mouse lung tissue lysate (negative) (40 µg/Lane)<br />Lane 3: Rat brain tissue lysate (40 µg/Lane)<br />Lane 4: Rat lung tissue lysate (negative) (40 µg/Lane)<br /><br />Predicted band size: 201 kDa<br />Observed band size: 159 kDa<br /><br />Exposure time: 40 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723226" style="font-weight: bold;text-decoration: underline;">HA723226</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of SHANK2 on different lysates with Rabbit anti-SHANK2 antibody (HA723226) at 1/2,000 dilution.

    Lane 1: Mouse brain tissue lysate (40 µg/Lane)
    Lane 2: Mouse lung tissue lysate (negative) (40 µg/Lane)
    Lane 3: Rat brain tissue lysate (40 µg/Lane)
    Lane 4: Rat lung tissue lysate (negative) (40 µg/Lane)

    Predicted band size: 201 kDa
    Observed band size: 159 kDa

    Exposure time: 40 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723226) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-SHANK2 antibody (<a href="/products/HA723226" style="font-weight: bold;text-decoration: underline;">HA723226</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723226" style="font-weight: bold;text-decoration: underline;">HA723226</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-SHANK2 antibody (HA723226) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723226) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-SHANK2 antibody (<a href="/products/HA723226" style="font-weight: bold;text-decoration: underline;">HA723226</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723226" style="font-weight: bold;text-decoration: underline;">HA723226</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-SHANK2 antibody (HA723226) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723226) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-SHANK2 antibody (<a href="/products/HA723226" style="font-weight: bold;text-decoration: underline;">HA723226</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723226" style="font-weight: bold;text-decoration: underline;">HA723226</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-SHANK2 antibody (HA723226) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723226) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-SHANK2 antibody (<a href="/products/HA723226" style="font-weight: bold;text-decoration: underline;">HA723226</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723226" style="font-weight: bold;text-decoration: underline;">HA723226</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-SHANK2 antibody (HA723226) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723226) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunofluorescence analysis of paraffin-embedded mouse hippocampus tissue labeling SHANK2 with Rabbit anti-SHANK2 antibody (<a href="/products/HA723226" style="font-weight: bold;text-decoration: underline;">HA723226</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA723226" style="font-weight: bold;text-decoration: underline;">HA723226</a>, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of paraffin-embedded mouse hippocampus tissue labeling SHANK2 with Rabbit anti-SHANK2 antibody (HA723226) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA723226, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

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