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BLBP Recombinant Rabbit Monoclonal Antibody [PSH10-68]

Usd: 385

Specification

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Catalog# HA723232

BLBP Recombinant Rabbit Monoclonal Antibody [PSH10-68]

Application

  • WB

  • IHC-P

  • IHC-Fr

  • IF-Tissue

Reactivity

  • Mouse

  • Rat

BSA and Azide free
Predicted reactivity

  • Cynomolgus monkey

  • Pig

Predicted species support after-sales service
Conjugation

  • unconjugated

Overview

Product Name

BLBP Recombinant Rabbit Monoclonal Antibody [PSH10-68]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Mouse BLBP aa 1-132.

Species Reactivity

Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

Predicted species support after-sales service

Validated Applications

WB, IHC-P, IHC-Fr, IF-Tissue

Molecular Weight

Predicted band size: 15 kDa

Positive Control

Mouse P7 brain tissue, mouse cerebellum tissue, Mouse cerebellum (P0) tissue lysate, Mouse cerebellum (P7) tissue lysate, Rat cerebellum tissue lysate.

Conjugation

unconjugated

Clone Number

PSH10-68

Reactivity Data

WB IHC-P IHC-Fr IF-Tissue
Mouse
Tested Tested
Tested Tested
Tested Tested
Rat
Tested Tested
Not Recommended
Cynomolgus Monkey
Published
Published
Published
Published
Pig
Published
Published
Published
Published

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:2,000

  • IHC-Fr

  • 1:500

  • IF-Tissue

  • 1:1,000

Target

Function

Fatty acid binding protein 7, brain (FABP7; also brain lipid binding protein, BLBP), is a human gene. The protein encoded by this gene is a brain fatty acid binding protein. Fatty acid binding proteins (FABPs) are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABPs are thought to play roles in fatty acid uptake, transport, and metabolism. FABP7 is expressed, during development, in radial glia by the activation of Notch receptors. Reelin was shown to induce FABP7 expression in neural progenitor cells via Notch-1 activation. According to one study, FABP7 binds DHA with the highest affinity among all of the FABPs.

Background References

1. Sabnis DH et al. BLBP Is Both a Marker for Poor Prognosis and a Potential Therapeutic Target in Paediatric Ependymoma. Cancers (Basel). 2021 Apr

2. Holst CB et al. The HOPX and BLBP landscape and gliogenic regions in developing human brain. J Anat. 2023 Jul

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: P51880 Mouse

SwissProt: P55051 Rat

Synonyms

B FABP antibody

B-FABP antibody

BFABP antibody

BLBP antibody

Brain lipid binding protein antibody

Brain lipid-binding protein antibody

Brain-type fatty acid-binding protein antibody

DKFZp547J2313 antibody

FABP 7 antibody

FABP7 antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebellum<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1:500

    Antigen retrieval: Not required

  • Application: IF-tissue<br /><br />Species: Mouse<br /><br />Site: Cerebral cortex (P7)<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1:1,000

    Application: IF-tissue

    Species: Mouse

    Site: Cerebral cortex (P7)

    Sample: Paraffin-embedded section

    Antibody concentration: 1:1,000

  • Application: IF-tissue<br /><br />Species: Mouse<br /><br />Site: Cerebellum<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1:1,000

    Application: IF-tissue

    Species: Mouse

    Site: Cerebellum

    Sample: Paraffin-embedded section

    Antibody concentration: 1:1,000

  • Immunohistochemical analysis of paraffin-embedded mouse P7 brain tissue with Rabbit anti-BLBP antibody (<a href="/products/HA723232" style="font-weight: bold;text-decoration: underline;">HA723232</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723232" style="font-weight: bold;text-decoration: underline;">HA723232</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse P7 brain tissue with Rabbit anti-BLBP antibody (HA723232) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723232) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse P7 brain tissue with Rabbit anti-BLBP antibody (<a href="/products/HA723232" style="font-weight: bold;text-decoration: underline;">HA723232</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723232" style="font-weight: bold;text-decoration: underline;">HA723232</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse P7 brain tissue with Rabbit anti-BLBP antibody (HA723232) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723232) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-BLBP antibody (<a href="/products/HA723232" style="font-weight: bold;text-decoration: underline;">HA723232</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723232" style="font-weight: bold;text-decoration: underline;">HA723232</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-BLBP antibody (HA723232) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723232) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of BLBP on different lysates with Rabbit anti-BLBP antibody (<a href="/products/HA723232" style="font-weight: bold;text-decoration: underline;">HA723232</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse cerebellum (P0) tissue lysate<br />Lane 2: Mouse cerebellum (P7) tissue lysate<br />Lane 3: Mouse kidney tissue lysate (negative)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 15 kDa<br />Observed band size: 15 kDa<br /><br />Exposure time: 14 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723232" style="font-weight: bold;text-decoration: underline;">HA723232</a>) at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of BLBP on different lysates with Rabbit anti-BLBP antibody (HA723232) at 1/5,000 dilution.

    Lane 1: Mouse cerebellum (P0) tissue lysate
    Lane 2: Mouse cerebellum (P7) tissue lysate
    Lane 3: Mouse kidney tissue lysate (negative)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 15 kDa
    Observed band size: 15 kDa

    Exposure time: 14 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723232) at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of BLBP on different lysates with Rabbit anti-BLBP antibody (<a href="/products/HA723232" style="font-weight: bold;text-decoration: underline;">HA723232</a>) at 1/5,000 dilution.<br /><br />Lane 1: Rat cerebellum tissue lysate<br />Lane 2: Rat kidney tissue lysate (negative)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 15 kDa<br />Observed band size: 15 kDa<br /><br />Exposure time: 59 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723232" style="font-weight: bold;text-decoration: underline;">HA723232</a>) at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of BLBP on different lysates with Rabbit anti-BLBP antibody (HA723232) at 1/5,000 dilution.

    Lane 1: Rat cerebellum tissue lysate
    Lane 2: Rat kidney tissue lysate (negative)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 15 kDa
    Observed band size: 15 kDa

    Exposure time: 59 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723232) at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

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