Human IL-18BP Recombinant Rabbit Monoclonal Antibody [PSH10-72] - BSA and Azide free (Detector)
Overview
Product Name
Human IL-18BP Recombinant Rabbit Monoclonal Antibody [PSH10-72] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human IL-18BP aa 31-194 (HA210686).
Species Reactivity
Human
Validated Applications
ELISA(Det)
Positive Control
Recombinant Human IL-18BP protein (HA210686).
Conjugation
unconjugated
Clone Number
PSH10-72
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH10-71] to Human IL-18BP antibody (Capture) (HA723235) and Recombinant Human IL-18BP protein (HA210686) as the standard. The reference range value is 39-5,000 pg/ml.
Target
Function
The protein encoded by this gene functions as an inhibitor of the proinflammatory cytokine, IL18. It binds IL18, prevents the binding of IL18 to its receptor, and thus inhibits IL18-induced IFN-gamma production, resulting in reduced T-helper type 1 immune responses. This protein is constitutively expressed and secreted in mononuclear cells. Elevated level of this protein is detected in the intestinal tissues of patients with Crohn's disease. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. Isoform A binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response. An autosomal recessive form of fulminant viral hepatitis, a disease that strikes otherwise healthy individuals during primary infection with common liver-tropic viruses. FVH is characterized by severe liver destruction in the absence of a preexisting liver disorder, leading to encephalopathy within 8 weeks of the onset of the first symptoms.
Background References
1. Belkaya S., Michailidis E., Korol C.B., Kabbani M., Cobat A., Bastard P., Lee Y.S., Hernandez N., Drutman S., Casanova J.L. Inherited IL-18BP deficiency in human fulminant viral hepatitis. J. Exp. Med. 216:1777-1790 (2019)
2. Kim S.-H., Eisenstein M., Reznikov L., Fantuzzi G., Novick D., Rubinstein M., Dinarello C.A. Structural requirements of six naturally occurring isoforms of the IL-18 binding protein to inhibit IL-18. Proc. Natl. Acad. Sci. U.S.A. 97:1190-1195 (2000)
Subcellular Location
Secreted.
UNIPROT
Synonyms
I18BP_HUMAN antibody
IL-18BP antibody
IL18 binding protein antibody
IL18 BP antibody
IL18 BPa antibody
IL18BP antibody
IL18BPa antibody
Interleukin 18 binding protein antibody
Interleukin-18-binding protein antibody
MC51L 53L 54L homolog gene product antibody
ExpandImages
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Sandwich ELISA analysis of human IL-18BP matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723235) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-18BP protein (HA210686) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723236, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
Interpolated concentrations of native IL-18BP in human urine samples.
The concentrations of IL-18BP were measured in duplicates, interpolated from the IL-18BP standard curve and corrected for sample dilution. Undiluted samples are human urine 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-18BP concentration was determined to be 56,552 pg/ml in human urine. -
Interpolated concentrations of spiked IL-18BP in human cell culture media samples.
The concentrations of IL-18BP were measured in duplicates, interpolated from the IL-18BP standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Human IL-18BP Recombinant Rabbit Monoclonal Antibody [PSH10-71] - BSA and Azide free (Capture)
Application: ELISA(Cap)
Reactivity: Human
Conjugate: unconjugated
