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DFNA5 / GSDME Recombinant Rabbit Monoclonal Antibody [PSH10-84]

Usd: 385

Specification

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Catalog# HA723251

DFNA5 / GSDME Recombinant Rabbit Monoclonal Antibody [PSH10-84]

Application

  • WB

  • IP

Reactivity

  • Human

  • Mouse

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

DFNA5 / GSDME Recombinant Rabbit Monoclonal Antibody [PSH10-84]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human GSDME aa 1-496.

Species Reactivity

Human, Mouse

Validated Applications

WB, IP

Molecular Weight

Predicted band size: 55 kDa

Positive Control

SH-SY5Y cell lysate, SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate.

Conjugation

unconjugated

Clone Number

PSH10-84

Reactivity Data

WB IP
Human
Tested Tested
Tested Tested
Mouse
Tested Tested
Rat
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IP

  • 1-2μg/sample

Target

Function

Non-syndromic hearing impairment protein 5 is a protein that in humans is encoded by the DFNA5 gene. Hearing impairment is a heterogeneous condition with over 40 loci described. The protein encoded by this gene is expressed in fetal cochlea, however, its function is not known. Nonsyndromic hearing impairment is associated with a mutation in this gene. The observation that DFNA5 is epigenetically inactivated in a large number of cancers of frequent types (gastric, colorectal, and breast) is another important finding and is in line with its apoptosis-inducing properties. Indeed, if apoptosis is an intrinsic feature of DFNA5, shutting the gene down in tumor cells makes them more susceptible to uncontrolled cellular growth. Moreover, the fact that DFNA5 is regulated by P53 strongly suggests that DFNA5 is a tumor suppressor gene.

Background References

1. Wei Y et al. GSDME-mediated pyroptosis promotes the progression and associated inflammation of atherosclerosis. Nat Commun. 2023 Feb

2. Ai YL et al. Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P. Cell Res. 2023 Dec

Subcellular Location

Cytoplasm, cytosol.

UNIPROT

SwissProt: O60443 Human

SwissProt: Q9Z2D3 Mouse

Synonyms

2310037D07Rik antibody

4932441K13Rik antibody

Deafness, autosomal dominant 5 antibody

Deafness, autosomal dominant 5 protein antibody

DFNA5 antibody

DFNA5 gene antibody

DFNA5_HUMAN antibody

Dfna5h antibody

EG14210 antibody

Fin15 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT),Relative expression (RE)</span><br /><br />Western blot analysis of DFNA5 / GSDME on different lysates with Rabbit anti-DFNA5 / GSDME antibody (<a href="/products/HA723251" style="font-weight: bold;text-decoration: underline;">HA723251</a>) at 1/2,000 dilution.<br /><br />Lane 1: SH-SY5Y cell lysate<br />Lane 2: SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate<br />Lane 3: SW480 cell lysate (negative)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 55 kDa<br />Observed band size: 55/30 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723251" style="font-weight: bold;text-decoration: underline;">HA723251</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT),Relative expression (RE)

    Western blot analysis of DFNA5 / GSDME on different lysates with Rabbit anti-DFNA5 / GSDME antibody (HA723251) at 1/2,000 dilution.

    Lane 1: SH-SY5Y cell lysate
    Lane 2: SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate
    Lane 3: SW480 cell lysate (negative)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 55 kDa
    Observed band size: 55/30 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723251) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of DFNA5 / GSDME on different lysates with Rabbit anti-DFNA5 / GSDME antibody (<a href="/products/HA723251" style="font-weight: bold;text-decoration: underline;">HA723251</a>) at 1/2,000 dilution.<br /><br />Lane 1: 293T transfected with empty control cell lysate<br />Lane 2: 293T transfected with mouse GSDME (N-terminal) cell lysate<br />Lane 3: 293T transfected with mouse GSDME (C-terminal) cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Exposure time: 40 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723251" style="font-weight: bold;text-decoration: underline;">HA723251</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of DFNA5 / GSDME on different lysates with Rabbit anti-DFNA5 / GSDME antibody (HA723251) at 1/2,000 dilution.

    Lane 1: 293T transfected with empty control cell lysate
    Lane 2: 293T transfected with mouse GSDME (N-terminal) cell lysate
    Lane 3: 293T transfected with mouse GSDME (C-terminal) cell lysate

    Lysates/proteins at 10 µg/Lane.

    Exposure time: 40 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723251) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />DFNA5 / GSDME was immunoprecipitated from 0.2 mg SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate with <a href="/products/HA723251" style="font-weight: bold;text-decoration: underline;">HA723251</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA723251" style="font-weight: bold;text-decoration: underline;">HA723251</a> at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (<a href="/products/M1208-2" style="font-weight: bold;text-decoration: underline;">M1208-2</a>) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate (input)<br />Lane 2: <a href="/products/HA723251" style="font-weight: bold;text-decoration: underline;">HA723251</a> IP in SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA723251" style="font-weight: bold;text-decoration: underline;">HA723251</a> in SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 10 seconds; ECL: K1801

    ☑ Cell treatment (CT)

    DFNA5 / GSDME was immunoprecipitated from 0.2 mg SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate with HA723251 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723251 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate (input)
    Lane 2: HA723251 IP in SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate
    Lane 3: Rabbit IgG instead of HA723251 in SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 10 seconds; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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