MAGI1 Recombinant Rabbit Monoclonal Antibody [PSH10-91]
Catalog# HA723258
MAGI1 Recombinant Rabbit Monoclonal Antibody [PSH10-91]
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WB
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Human
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Mouse
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Rat
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HA751382
不含抗保成分
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unconjugated
Overview
Product Name
MAGI1 Recombinant Rabbit Monoclonal Antibody [PSH10-91]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human MAGI1 aa 551-1,000.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 165 kDa
Positive Control
U-2 OS cell lysate, SW480 cell lysate, HepG2 cell lysate, HCT 116 cell lysate, HUVEC cell lysate, Mouse liver tissue lysate, Mouse pancreas tissue lysate, Rat pancreas tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
PSH10-91
Reactivity Data
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
Target
Function
Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 is an enzyme that in humans is encoded by the MAGI1 gene. The protein encoded by this gene is a member of the membrane-associated guanylate kinase homologue (MAGUK) family. MAGUK proteins participate in the assembly of multiprotein complexes on the inner surface of the plasma membrane at regions of cell–cell contact. The product of this gene may play a role as scaffolding protein at cell–cell junctions. Alternatively spliced transcript variants encoding different isoforms have been identified.
Background References
1. Wörthmüller J et al. MAGI1, a Scaffold Protein with Tumor Suppressive and Vascular Functions. Cells. 2021 Jun
2. Wang Y et al. MAGI1 inhibits interferon signaling to promote influenza A infection. Front Cardiovasc Med. 2022 Aug
Subcellular Location
Cell junction, tight junction, Cell membrane.
Synonyms
AIP 3 antibody
AIP-3 antibody
AIP3 antibody
Atrophin 1 interacting protein 3 antibody
Atrophin-1-interacting protein 3 antibody
BAI1 associated protein 1 antibody
BAI1-associated protein 1 antibody
BAIAP 1 antibody
BAIAP1 antibody
BAP 1 antibody
ExpandImages
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Western blot analysis of MAGI1 on different lysates with Rabbit anti-MAGI1 antibody (HA723258) at 1/2,000 dilution.
Lane 1: U-2 OS cell lysate (20 µg/Lane)
Lane 2: SW480 cell lysate (20 µg/Lane)
Lane 3: HepG2 cell lysate (20 µg/Lane)
Lane 4: HCT 116 cell lysate (20 µg/Lane)
Lane 5: HUVEC cell lysate (20 µg/Lane)
Lane 6: Mouse liver tissue lysate (40 µg/Lane)
Lane 7: Mouse pancreas tissue lysate (40 µg/Lane)
Lane 8: Rat pancreas tissue lysate (40 µg/Lane)
Lane 9: Mouse brain tissue lysate (40 µg/Lane)
Lane 10: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 165 kDa
Observed band size: 100-180 kDa
Exposure time: Lane 1-8: 2 minute 7 seconds; Lane 9-10: 25 seconds;ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723258) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"