HRP Conjugated Strep-Tag II Recombinant Rabbit Monoclonal Antibody [PSH05-13]
Catalog# HA723259
HRP Conjugated Strep-Tag II Recombinant Rabbit Monoclonal Antibody [PSH05-13]
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WB
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Species independent
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HRP
Overview
Product Name
HRP Conjugated Strep-Tag II Recombinant Rabbit Monoclonal Antibody [PSH05-13]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide corresponding to Strep-tag II.
Species Reactivity
Species independent
Validated Applications
WB
Conjugation
HRP
Clone Number
PSH05-13
Reactivity Data
| WB | |
|---|---|
| Species Independent |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
Target
Function
The Strep-tag® system is a method which allows the purification and detection of proteins by affinity chromatography. The Strep-tag II is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin®, a specifically engineered streptavidin, and can be N- or C- terminally fused to recombinant proteins. By exploiting the highly specific interaction, Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions it is especially suited for generation of functional proteins.
Background References
1. Arne Skerra. et. al. The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins. Nat Protoc. 2007;2(6):1528-35.
Synonyms
anti-Strep-Tag II antibody
Strep-Tag II antibody
Strep-Tag II antibody antibody
Strep-Tag IIantiboy antibody
Images
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Western blot analysis of Strep-Tag II on different lysates with Rabbit anti-Strep-Tag II antibody (HA723259) at 1/2,000 dilution.
Lane 1: 293T cell lysate (10 µg/Lane)
Lane 2: 293T transfected with Strep-Tag II-tagged Histone H3.1 (C-terminal) cell lysate (20 µg/Lane)
Lane 3: 293T transfected with Strep-Tag II-tagged ACAT2 (N-terminal) cell lysate (10 µg/Lane)
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723259) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"