FOXM1 Recombinant Rabbit Monoclonal Antibody [PSH10-93]
Catalog# HA723260
FOXM1 Recombinant Rabbit Monoclonal Antibody [PSH10-93]
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IHC-P
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IF-Cell
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Human
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Mouse
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HA751383
不含抗保成分
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unconjugated
Overview
Product Name
FOXM1 Recombinant Rabbit Monoclonal Antibody [PSH10-93]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human FOXM1 aa 1-400.
Species Reactivity
Human, Mouse
Validated Applications
IHC-P, IF-Cell
Molecular Weight
Predicted band size: 84 kDa
Positive Control
Human colon tissue, human colon cancer tissue, HeLa, MEF.
Conjugation
unconjugated
Clone Number
PSH10-93
Reactivity Data
| IHC-P | IF-Cell | |
|---|---|---|
| Human |
|
|
| Mouse |
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-P
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1:1,000
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IF-Cell
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1:100
Target
Function
Forkhead box protein M1 is a protein that in humans is encoded by the FOXM1 gene. The protein encoded by this gene is a member of the FOX family of transcription factors. FOXM1 is known to play a key role in cell cycle progression where endogenous FOXM1 expression peaks at S and G2/M phases. FOXM1-null mouse embryos were neonatal lethal as a result of the development of polyploid cardiomyocytes and hepatocytes, highlighting the role of FOXM1 in mitotic division. More recently a study using transgenic/knockout mouse embryonic fibroblasts and human osteosarcoma cells (U2OS) has shown that FOXM1 regulates expression of a large array of G2/M-specific genes, such as Plk1, cyclin B2, Nek2 and CENPF, and plays an important role in maintenance of chromosomal segregation and genomic stability.
Background References
1. Khan MA et al. FOXM1: A small fox that makes more tracks for cancer progression and metastasis. Semin Cancer Biol. 2023 Jul
2. Zhang Z et al. FOXM1: Functional Roles of FOXM1 in Non-Malignant Diseases. Biomolecules. 2023 May
Subcellular Location
Nucleus.
Synonyms
FKHL16 antibody
Forkhead box M1 antibody
Forkhead box protein M1 antibody
forkhead like 16 antibody
Forkhead-related protein FKHL16 antibody
FOX M1 antibody
Foxm1 antibody
FOXM1_HUMAN antibody
FOXM1B antibody
Hepatocyte nuclear factor 3 forkhead homolog 11 antibody
ExpandImages
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Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-FOXM1 antibody (HA723260) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723260) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-FOXM1 antibody (HA723260) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723260) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunocytochemistry analysis of HeLa cells labeling FOXM1 with Rabbit anti-FOXM1 antibody (HA723260) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FOXM1 antibody (HA723260) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of MEF cells labeling FOXM1 with Rabbit anti-FOXM1 antibody (HA723260) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FOXM1 antibody (HA723260) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"