IL-8 Recombinant Rabbit Monoclonal Antibody [PSH10-96]
Overview
Product Name
IL-8 Recombinant Rabbit Monoclonal Antibody [PSH10-96]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human IL-8 aa 1-99.
Species Reactivity
Human
Validated Applications
WB, FC
Molecular Weight
Predicted band size: 11 kDa
Positive Control
U-937 treated with 100ng/mL TPA for 24 hours then add 5μg/mL LPS for 4 hours then add 300ng/mL BFA for 3 hours cell lysate, U-937 treated with 100ng/mL TPA for 24 hours then add 5μg/mL LPS for 4 hours then add 300ng/mL BFA for 3 hours.
Conjugation
unconjugated
Clone Number
PSH10-96
Reactivity Data
| WB | FC | |
|---|---|---|
| Human |
|
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000
-
FC
-
1:1,000
Target
Function
Interleukin 8 (IL-8 or chemokine (C-X-C motif) ligand 8, CXCL8) is a chemokine produced by macrophages and other cell types such as epithelial cells, airway smooth muscle cells and endothelial cells. Endothelial cells store IL-8 in their storage vesicles, the Weibel–Palade bodies. In humans, the interleukin-8 protein is encoded by the CXCL8 gene. IL-8 is initially produced as a precursor peptide of 99 amino acids which then undergoes cleavage to create several active IL-8 isoforms. In culture, a 72 amino acid peptide is the major form secreted by macrophages. There are many receptors on the surface membrane capable of binding IL-8; the most frequently studied types are the G protein-coupled serpentine receptors CXCR1 and CXCR2. Expression and affinity for IL-8 differs between the two receptors (CXCR1 > CXCR2). Through a chain of biochemical reactions, IL-8 is secreted and is an important mediator of the immune reaction in the innate immune system response.
Background References
1. Ramachandra N et al. Role of IL8 in myeloid malignancies. Leuk Lymphoma. 2023 Nov-Dec
2. Jiang H et al. Targeting IL8 as a sequential therapy strategy to overcome chemotherapy resistance in advanced gastric cancer. Cell Death Discov. 2022 Apr
Subcellular Location
Secreted.
UNIPROT
Synonyms
(Ala-IL-8)77 antibody
(Ser-IL-8)72 antibody
9E3 antibody
Beta thromboglobulin like protein antibody
C-X-C motif chemokine 8 antibody
CEF-4 antibody
chemokine, CXC motif, ligand 8 antibody
CXCL8 antibody
Emoctakin antibody
GCP-1 antibody
ExpandImages
-
☑ Cell treatment (CT)
Western blot analysis of IL-8 on different lysates with Rabbit anti-IL-8 antibody (HA723263) at 1/2,000 dilution.
Lane 1: U-937 cell lysate
Lane 2: U-937 treated with 100ng/mL TPA for 24 hours then add 5μg/mL LPS for 4 hours then add 300ng/mL BFA for 3 hours cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 11 kDa
Observed band size: 11 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723263) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Flow cytometric analysis of U-937 cells untreated (left) / treated with 100ng/mL TPA for 24 hours then add 5μg/mL LPS for 4 hours then add 300ng/mL BFA for 3 hours (right) labeling IL-8.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA723263, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
-
Aldometanib mitigates LPS-induced Lung fibroblasts injury through the activation of AMPK-mediated signaling pathways
Journal: Toxicon
DOI: 10.1016/j.toxicon.2025.108489
IF: 2.4
Application: WB
Reactivity: Human
Publish date: 2025 Jul
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