Presenilin 2 / AD5 Recombinant Rabbit Monoclonal Antibody [PSH11-18]
Overview
Product Name
Presenilin 2 / AD5 Recombinant Rabbit Monoclonal Antibody [PSH11-18]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human Presenilin 2 aa 301-350.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, FC, IP
Molecular Weight
Predicted band size: 50 kDa
Positive Control
HeLa cell lysate, SH-SY5Y cell lysate, RAW264.7 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, PC-12.
Conjugation
unconjugated
Clone Number
PSH11-18
Reactivity Data
| WB | IF-Cell | FC | IP | |
|---|---|---|---|---|
| Human |
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| Mouse |
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| Rat |
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Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000
-
IF-Cell
-
1:100
-
FC
-
1:1,000
-
IP
-
1-2μg/sample
Target
Function
Presenilin-2 is a protein that (in humans) is encoded by the PSEN2 gene. Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1; PSEN2) or the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor, such that they either directly regulate gamma-secretase activity or themselves are protease enzymes. Two alternative transcripts of PSEN2 have been identified. In melanocytic cells PSEN2 gene expression may be regulated by MITF.
Background References
1. Pizzo P et al. Presenilin-2 and Calcium Handling: Molecules, Organelles, Cells and Brain Networks. Cells. 2020 Sep
2. Yoo SS et al. Presenilin-2 knock-In mice show severe depressive behavior via DVL3 downregulation. CNS Neurosci Ther. 2024 Feb
Subcellular Location
Endoplasmic reticulum membrane, Golgi apparatus membrane.
Synonyms
AD3L antibody
AD3LP antibody
AD4 antibody
AD5 antibody
Alzheimer disease 4 antibody
CMD1V antibody
E5-1 antibody
OTTHUMP00000035671 antibody
OTTHUMP00000035672 antibody
OTTHUMP00000228286 antibody
ExpandImages
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Western blot analysis of Presenilin 2 / AD5 on different lysates with Rabbit anti-Presenilin 2 / AD5 antibody (HA723300) at 1/2,000 dilution.
Lane 1: HeLa cell lysate (no heat)
Lane 2: SH-SY5Y cell lysate (no heat)
Lane 3: RAW264.7 cell lysate (no heat)
Lane 4: Neuro-2a cell lysate (no heat)
Lane 5: PC-12 cell lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 23 kDa
Exposure time: Lane 1-4: 3 minutes; Lane 3: 1 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723300) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of PC-12 cells labeling Presenilin 2 / AD5 with Rabbit anti-Presenilin 2 / AD5 antibody (HA723300) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Presenilin 2 / AD5 antibody (HA723300) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of PC-12 cells labeling Presenilin 2 / AD5.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA723300, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Presenilin 2 / AD5 was immunoprecipitated from 0.2 mg SH-SY5Y cell lysate with HA723300 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723300 at 1/1,000 dilution. Mouse anti Rabbit IgG heavy chain (Fc) secondary antibody (M1003-7) at 1/10,000 dilution was used for 1 hour at room temperature.
Lane 1: SH-SY5Y cell lysate (input)
Lane 2: HA723300 IP in SH-SY5Y cell lysate
Lane 3: Rabbit IgG instead of HA723300 in SH-SY5Y cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 3 minutes; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
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Targeting SHMT2-mediated membrane phospholipid remodeling for enhanced anti-GCSCs treatment
Journal: Interdisciplinary Medicine
DOI: 10.1002/INMD.20240106
IF: 13.6
Application: WB
Reactivity: Human
Publish date: 2025 Apr