Presenilin 1 / PS-1 Recombinant Rabbit Monoclonal Antibody [PSH11-19]
Overview
Product Name
Presenilin 1 / PS-1 Recombinant Rabbit Monoclonal Antibody [PSH11-19]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human Presenilin 1 aa 311-360.
Product Specificity
Presenilin 1 / PS-1 [PSH11-19] Rabbit mAb detects endogenous levels of the CTF (carboxy-terminal fragment) of presenilin 1 (22 kDa).
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IP
Molecular Weight
Predicted band size: 53 kDa
Positive Control
HeLa cell lysate, HEK-293 cell lysate, MEF cell lysate, HepG2 cell lysate, HL-60 cell lysate, Daudi cell lysate, Neuro-2a cell lysate, Mouse brain tissue lysate, Mouse cerebellum tissue lysate, Rat brain tissue lysate, Rat cerebellum tissue lysate.
Conjugation
unconjugated
Clone Number
PSH11-19
Reactivity Data
| WB | IP | |
|---|---|---|
| Human |
|
|
| Mouse |
|
|
| Rat |
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000
-
IP
-
1-2μg/sample
Target
Function
Presenilin-1 (PS-1) is a presenilin protein that in humans is encoded by the PSEN1 gene. Presenilin-1 is one of the four core proteins in the gamma secretase complex, which is considered to play an important role in generation of amyloid beta (Aβ) from amyloid-beta precursor protein (APP). Accumulation of amyloid beta is associated with the onset of Alzheimer's disease. Presenilins are postulated to regulate APP processing through their effects on gamma secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor, such that they either directly regulate gamma secretase activity or themselves are protease enzymes. Multiple alternatively spliced transcript variants have been identified for this gene, the full-length natures of only some have been determined.
Background References
1. Yang Y et al. Presenilin-1 (PSEN1) Mutations: Clinical Phenotypes beyond Alzheimer\'s Disease. Int J Mol Sci. 2023 May
2. Gamez-Belmonte R et al. Epithelial presenilin-1 drives colorectal tumour growth by controlling EGFR-COX2 signalling. Gut. 2023 Jun
Subcellular Location
Endoplasmic reticulum, Endoplasmic reticulum membrane, Golgi apparatus membrane, Cytoplasmic granule, Cell membrane, Cell projection, growth cone, Early endosome, Early endosome membrane, neuron projection, axon, Synapse.
Synonyms
AD3 antibody
Ad3h antibody
FAD antibody
Homo Sapiens Clone CC44 Senilin 1 antibody
Presenilin-1 CTF12 antibody
Protein S182 antibody
PS 1 antibody
PS-1 antibody
PS1-CTF12 antibody
PSEN1 antibody
ExpandImages
-
Western blot analysis of Presenilin 1 / PS-1 on different lysates with Rabbit anti-Presenilin 1 / PS-1 antibody (HA723301) at 1/2,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HEK-293 cell lysate (20 µg/Lane)
Lane 3: MEF cell lysate (20 µg/Lane)
Lane 4: HepG2 cell lysate (20 µg/Lane)
Lane 5: HL-60 cell lysate (20 µg/Lane)
Lane 6: Daudi cell lysate (20 µg/Lane)
Lane 7: Neuro-2a cell lysate (20 µg/Lane)
Lane 8: Mouse brain tissue lysate (40 µg/Lane)
Lane 9: Mouse cerebellum tissue lysate (40 µg/Lane)
Lane 10: Rat brain tissue lysate (40 µg/Lane)
Lane 11: Rat cerebellum tissue lysate (40 µg/Lane)
Predicted band size: 53 kDa
Observed band size: 18 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723301) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Presenilin 1 / PS-1 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA723301 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723301 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: HA723301 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of HA723301 in HeLa cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 10 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
-
Targeting SHMT2-mediated membrane phospholipid remodeling for enhanced anti-GCSCs treatment
Journal: Interdisciplinary Medicine
DOI: 10.1002/INMD.20240106
IF: 13.6
Application: WB
Reactivity: Human
Publish date: 2025 Apr
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