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Gli2 Recombinant Rabbit Monoclonal Antibody [PSH11-23]

Usd: 385

Specification

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Catalog# HA723305

Gli2 Recombinant Rabbit Monoclonal Antibody [PSH11-23]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

Gli2 Recombinant Rabbit Monoclonal Antibody [PSH11-23]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human Gli2 aa 571-830.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 168 kDa

Positive Control

K-562 cell lysate, HT-29 cell lysate, F9 cell lysate, Mouse colon tissue lysate, Rat pancreas tissue lysate, Rat colon tissue lysate.

Conjugation

unconjugated

Clone Number

PSH11-23

Reactivity Data

WB
Human
Tested Tested
Mouse
Tested Tested
Rat
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

Target

Function

Zinc finger protein GLI2 also known as GLI family zinc finger 2 is a protein that in humans is encoded by the GLI2 gene. The protein encoded by this gene is a transcription factor. GLI2 belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis.

Background References

1. Chen Z et al. SHH/GLI2-TGF-beta1 feedback loop between cancer cells and tumor-associated macrophages maintains epithelial-mesenchymal transition and endoplasmic reticulum homeostasis in cholangiocarcinoma. Pharmacol Res. 2023 Jan

2. Huang A et al. Shh-Gli2-Runx2 inhibits vascular calcification. Nephrol Dial Transplant. 2024 Jan

Subcellular Location

Nucleus, Cytoplasm, Cell projection, cilium.

UNIPROT

SwissProt: P10070 Human

SwissProt: Q0VGT2 Mouse

Entrez Gene: 304729 Rat

Synonyms

CJS antibody

Gli 2 antibody

GLI family zinc finger 2 antibody

GLI Kruppel family member GLI2 antibody

GLI2 antibody

GLI2_HUMAN antibody

Glioma associated oncogene family zinc finger antibody

HPE9 antibody

Oncogene GLI2 antibody

PHS2 antibody

Expand

Images

  • Western blot analysis of Gli2 on different lysates with Rabbit anti-Gli2 antibody (<a href="/products/HA723305" style="font-weight: bold;text-decoration: underline;">HA723305</a>) at 1/2,000 dilution.<br /><br />Lane 1: K-562 cell lysate (20 µg/Lane)<br />Lane 2: HT-29 cell lysate (20 µg/Lane)<br />Lane 3: F9 cell lysate (20 µg/Lane)<br />Lane 4: Mouse colon tissue lysate (40 µg/Lane)<br />Lane 5: Rat pancreas tissue lysate (40 µg/Lane)<br />Lane 6: Rat colon tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 168 kDa<br />Observed band size: 133 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723305" style="font-weight: bold;text-decoration: underline;">HA723305</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Gli2 on different lysates with Rabbit anti-Gli2 antibody (HA723305) at 1/2,000 dilution.

    Lane 1: K-562 cell lysate (20 µg/Lane)
    Lane 2: HT-29 cell lysate (20 µg/Lane)
    Lane 3: F9 cell lysate (20 µg/Lane)
    Lane 4: Mouse colon tissue lysate (40 µg/Lane)
    Lane 5: Rat pancreas tissue lysate (40 µg/Lane)
    Lane 6: Rat colon tissue lysate (40 µg/Lane)

    Predicted band size: 168 kDa
    Observed band size: 133 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723305) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"