Gli2 Recombinant Rabbit Monoclonal Antibody [PSH11-23]
Catalog# HA723305
Gli2 Recombinant Rabbit Monoclonal Antibody [PSH11-23]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Gli2 Recombinant Rabbit Monoclonal Antibody [PSH11-23]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Gli2 aa 571-830.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 168 kDa
Positive Control
K-562 cell lysate, HT-29 cell lysate, F9 cell lysate, Mouse colon tissue lysate, Rat pancreas tissue lysate, Rat colon tissue lysate.
Conjugation
unconjugated
Clone Number
PSH11-23
Reactivity Data
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
Product Features
Form
Liquid
Concentration
0.2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:2,000
Target
Function
Zinc finger protein GLI2 also known as GLI family zinc finger 2 is a protein that in humans is encoded by the GLI2 gene. The protein encoded by this gene is a transcription factor. GLI2 belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis.
Background References
1. Chen Z et al. SHH/GLI2-TGF-beta1 feedback loop between cancer cells and tumor-associated macrophages maintains epithelial-mesenchymal transition and endoplasmic reticulum homeostasis in cholangiocarcinoma. Pharmacol Res. 2023 Jan
2. Huang A et al. Shh-Gli2-Runx2 inhibits vascular calcification. Nephrol Dial Transplant. 2024 Jan
Subcellular Location
Nucleus, Cytoplasm, Cell projection, cilium.
Synonyms
CJS antibody
Gli 2 antibody
GLI family zinc finger 2 antibody
GLI Kruppel family member GLI2 antibody
GLI2 antibody
GLI2_HUMAN antibody
Glioma associated oncogene family zinc finger antibody
HPE9 antibody
Oncogene GLI2 antibody
PHS2 antibody
ExpandImages
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Western blot analysis of Gli2 on different lysates with Rabbit anti-Gli2 antibody (HA723305) at 1/2,000 dilution.
Lane 1: K-562 cell lysate (20 µg/Lane)
Lane 2: HT-29 cell lysate (20 µg/Lane)
Lane 3: F9 cell lysate (20 µg/Lane)
Lane 4: Mouse colon tissue lysate (40 µg/Lane)
Lane 5: Rat pancreas tissue lysate (40 µg/Lane)
Lane 6: Rat colon tissue lysate (40 µg/Lane)
Predicted band size: 168 kDa
Observed band size: 133 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723305) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"