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Lipoprotein a Recombinant Rabbit Monoclonal Antibody [PSH11-29]

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Specification

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Catalog# HA723311

Lipoprotein a Recombinant Rabbit Monoclonal Antibody [PSH11-29]

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

Lipoprotein a Recombinant Rabbit Monoclonal Antibody [PSH11-29]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human LPA aa 1-1,050.

Species Reactivity

Human

Validated Applications

WB, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 227 kDa

Positive Control

Human serum lysate, Human plasma lysate, human liver tissue, human glioma tumor tissue.

Conjugation

unconjugated

Clone Number

PSH11-29

Reactivity Data

WB IHC-P IF-Tissue
Human
Tested Tested
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:1,000

  • IF-Tissue

  • 1:200

Target

Function

Lipoprotein(a) is a low-density lipoprotein variant containing a protein called apolipoprotein(a). Genetic and epidemiological studies have identified lipoprotein(a) as a risk factor for atherosclerosis and related diseases, such as coronary heart disease and stroke. Lp(a) is assembled at the hepatocyte cell membrane surface, which is similar to typical LDL particles. However, there are other possible locations of assembly. The particles mainly exist in plasma. Lp(a) contributes to the process of atherogenesis. The structure of apolipoprotein(a) is similar to plasminogen and tPA (tissue plasminogen activator) and it competes with plasminogen for its binding site, leading to reduced fibrinolysis. Also, because Lp(a) stimulates secretion of PAI-1, it leads to thrombogenesis. It also may enhance coagulation by inhibiting the function of tissue factor pathway inhibitor.

Background References

1. Duarte Lau F et al. Lipoprotein(a) and its Significance in Cardiovascular Disease: A Review. JAMA Cardiol. 2022 Jul

2. Lampsas S et al. Lipoprotein(a) in Atherosclerotic Diseases: From Pathophysiology to Diagnosis and Treatment. Molecules. 2023 Jan

Subcellular Location

Extracellular region, extracellular space, plasma lipoprotein particle.

UNIPROT

SwissProt: P08519 Human

Synonyms

AK38 antibody

Antiangiogenic AK38 protein antibody

apo(a) antibody

APOA antibody

apolipoprotein(a) antibody

LP antibody

lp(a) antibody

LPA antibody

Lipoprotein a

Images

  • Western blot analysis of Lipoprotein a on different lysates with Rabbit anti-Lipoprotein a antibody (<a href="/products/HA723311" style="font-weight: bold;text-decoration: underline;">HA723311</a>) at 1/2,000 dilution.<br /><br />Lane 1: Human serum lysate<br />Lane 2: Human plasma lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 227 kDa<br />Observed band size: 500 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br />3-8% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723311" style="font-weight: bold;text-decoration: underline;">HA723311</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Lipoprotein a on different lysates with Rabbit anti-Lipoprotein a antibody (HA723311) at 1/2,000 dilution.

    Lane 1: Human serum lysate
    Lane 2: Human plasma lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 227 kDa
    Observed band size: 500 kDa

    Exposure time: 10 seconds; ECL: K1801;
    3-8% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723311) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Lipoprotein a antibody (<a href="/products/HA723311" style="font-weight: bold;text-decoration: underline;">HA723311</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723311" style="font-weight: bold;text-decoration: underline;">HA723311</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Lipoprotein a antibody (HA723311) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723311) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human glioma tumor tissue with Rabbit anti-Lipoprotein a antibody (<a href="/products/HA723311" style="font-weight: bold;text-decoration: underline;">HA723311</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723311" style="font-weight: bold;text-decoration: underline;">HA723311</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human glioma tumor tissue with Rabbit anti-Lipoprotein a antibody (HA723311) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723311) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded human brain tissue (negative) with Rabbit anti-Lipoprotein a antibody (<a href="/products/HA723311" style="font-weight: bold;text-decoration: underline;">HA723311</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723311" style="font-weight: bold;text-decoration: underline;">HA723311</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded human brain tissue (negative) with Rabbit anti-Lipoprotein a antibody (HA723311) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723311) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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