Bik Recombinant Rabbit Monoclonal Antibody [PSH11-51]
Catalog# HA723343
Bik Recombinant Rabbit Monoclonal Antibody [PSH11-51]
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WB
-
IF-Cell
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IP
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Human
-
unconjugated
Overview
Product Name
Bik Recombinant Rabbit Monoclonal Antibody [PSH11-51]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Bik aa 1-160.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IP
Target Molecular Weight
Predicted band size: 18 kDa
Positive Control
Ramos cell lysate, Raji cell lysate, THP-1 cell lysate, Ramos.
Conjugation
unconjugated
Clone Number
PSH11-51
Reactivity Data
| WB | IF-Cell | IP | |
|---|---|---|---|
| Human |
|
|
|
| Mouse |
|
||
| Rat |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:10,000
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IF-Cell
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1:100
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IP
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1-2μg/sample
Target
Function
Bcl-2-interacting killer is a protein that in humans is encoded by the BIK gene. The protein encoded by this gene is known to interact with cellular and viral survival-promoting proteins, such as BCL2 and the Epstein–Barr virus in order to enhance programmed cell death. Because its activity is suppressed in the presence of survival-promoting proteins, this protein is suggested as a likely target for antiapoptotic proteins. This protein shares a critical BH3 domain with other death-promoting proteins, BAX and BAK.
Background References
1. Mebratu YA et al. Bik promotes proteasomal degradation to control low-grade inflammation. J Clin Invest. 2023 Dec
2. Zhang P et al. TMEM215 Prevents Endothelial Cell Apoptosis in Vessel Regression by Blunting BIK-Regulated ER-to-Mitochondrial Ca Influx. Circ Res. 2023 Oct
Subcellular Location
Endomembrane system, Mitochondrion membrane.
UNIPROT
Synonyms
Apoptosis inducer NBK antibody
BBC1 antibody
Bcl-2-interacting killer antibody
BCL2 interacting killer antibody
bhikhari antibody
BIK antibody
Bik-like killer protein antibody
BIK_HUMAN antibody
BIP 1 antibody
BiP1 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of Bik on different lysates with Rabbit anti-Bik antibody (HA723343) at 1/10,000 dilution.
Lane 1: Ramos cell lysate
Lane 2: SK-Br-3 cell lysate (negative)
Lane 3: Raji cell lysate
Lane 4: THP-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 18 kDa
Observed band size: 18 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723343) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of Ramos cells labeling Bik with Rabbit anti-Bik antibody (HA723343) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Bik antibody (HA723343) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Bik was immunoprecipitated from 0.2 mg Ramos cell lysate with HA723343 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723343 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: Ramos cell lysate (input)
Lane 2: HA723343 IP in Ramos cell lysate
Lane 3: Rabbit IgG instead of HA723343 in Ramos cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 20 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"