Nectin 2 Recombinant Rabbit Monoclonal Antibody [PSH11-54]
Overview
Product Name
Nectin 2 Recombinant Rabbit Monoclonal Antibody [PSH11-54]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Nectin 2 aa 1-360.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, FC
Molecular Weight
Predicted band size: 58 kDa
Positive Control
MCF7 cell lysate, HT-29 cell lysate, MCF7.
Conjugation
unconjugated
Clone Number
PSH11-54
Reactivity Data
| WB | IF-Cell | FC | |
|---|---|---|---|
| Human |
|
|
|
| Mouse |
|
|
|
| Rat |
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000
-
IF-Cell
-
1:100
-
FC
-
1:1,000
Target
Function
Nectin is a Ca2+-independent homophilic cell adhesion molecule that belongs to the immunoglobulin superfamily. Human nectin is identical to the poliovirus receptor-related protein (PRR) and has been identified as the α-herpesvirus entry mediator. Nectin constitutes a family consisting of at least Nectin 1, 2 and 3; each member has two or three splicing variants. Nectin 2, also designated PRR2/HveB, is ubquitously expressed, with the highest levels of expression in some human neuronal cell lines, fibroblastic cells, keratinocytes and primary activated T lymphocytes. Nectin 2 has two splicing variants, Nectin 2α (short form) and 2δ (long form). Both Nectin 2α and 2δ have a C-terminal conserved motif (E/A-X-Y-V). This motif interacts with the PDZ domain of the F-Actin-binding protein afadin, through which it is linked to the Actin cytoskeleton. The extracellular regions of the splicing variants are identical, but their transmembrane regions and cytoplasmic regions are unique. Nectin 2 mediates the entry of three mutant herpes simplex virus type 1 (HSV-1) strains that do not use HveA as co-receptor, but not wildtype HSV-1 strains. Nectin 2 also mediates the entry of HSV-2 and pseudorabies virus, but not bovine herpes virus type 1. Nectin 2δ is tyrosine phosphorylated in response to cell-cell adhesion.
Background References
1. Wienke J et al. Integrative analysis of neuroblastoma by single-cell RNA sequencing identifies the NECTIN2-TIGIT axis as a target for immunotherapy. Cancer Cell. 2024 Feb
2. Agorku DJ et al. Colorectal cancer-associated fibroblasts inhibit effector T cells via NECTIN2 signaling. Cancer Lett. 2024 Jul
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
CD 112 antibody
CD112 antibody
CD112 antigen antibody
Herpes virus entry mediator B antibody
Herpes virus entry protein B antibody
Herpesvirus entry mediator B antibody
Herpesvirus entry protein B antibody
Hve B antibody
HveB antibody
Nectin-2 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of Nectin 2 on different lysates with Rabbit anti-Nectin 2 antibody (HA723346) at 1/2,000 dilution.
Lane 1: MCF7 cell lysate
Lane 2: MOLT-4 cell lysate (negative)
Lane 3: HT-29 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 58 kDa
Observed band size: 58-75 kDa
Exposure time: 25 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723346) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Relative expression (RE)
Immunocytochemistry analysis of MCF7 (positive) and MOLT-4 (negative) labeling Nectin 2 with Rabbit anti-Nectin 2 antibody (HA723346) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Nectin 2 antibody (HA723346) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
☑ Relative expression (RE)
Flow cytometric analysis of MOLT-4 (left, negative) and MCF7 (right, positive) cells labeling Nectin 2.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA723346, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
-
VSIG2 as a novel immunosuppressive ligand interacts with Nectin-2 to regulate T cell responses
Journal: Journal Of Neuroinflammation
DOI: 10.1186/s12974-025-03645-7
IF: 10.1
Application: WB
Reactivity: Mouse
Publish date: 2025 Dec