IFT88 Recombinant Rabbit Monoclonal Antibody [PSH11-61]
Catalog# HA723357
IFT88 Recombinant Rabbit Monoclonal Antibody [PSH11-61]
-
WB
-
IHC-P
-
IF-Cell
-
Human
-
Mouse
-
Rat
-
Green monkey
-
HA751417
不含抗保成分
-
unconjugated
Overview
Product Name
IFT88 Recombinant Rabbit Monoclonal Antibody [PSH11-61]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human IFT88 aa 575-824.
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB, IHC-P, IF-Cell
Molecular Weight
Predicted band size: 93 kDa
Positive Control
HEK-293 cell lysate, Jurkat cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, C6 cell lysate, A549 cell lysate, HepG2 cell lysate, COS-1 cell lysate, human testis tissue, rat testis tissue, NIH/3T3, C6.
Conjugation
unconjugated
Clone Number
PSH11-61
Reactivity Data
| WB | IHC-P | IF-Cell | |
|---|---|---|---|
| Human |
|
|
|
| Mouse |
|
|
|
| Rat |
|
|
|
| Green Monkey |
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000-1:5,000
-
IHC-P
-
1:1,000
-
IF-Cell
-
1:50
Target
Function
Intraflagellar transport protein 88 homolog is a protein that is encoded by the IFT88 gene. This gene encodes a member of the tetratrico peptide repeat (TPR) family. Mutations of a similar gene in mouse can cause polycystic kidney disease. Two transcript variants encoding distinct isoforms have been identified for this gene. In 2012 a mutation was found to be responsible for a novel form of ciliopathy and anosmia in humans capable of remedy in mice by adenoviral mediated gene therapy. IFT88 has been shown to interact with BAT2 and WDR62.[8][9] WDR62 is required for IFT88 localization to the cilia basal body and the cilia axoneme.
Background References
1. Kitamura A et al. Ift88 is involved in mandibular development. J Anat. 2020 Feb
2. Wang S et al. IFT88 deficiency in proximal tubular cells exaggerates cisplatin-induced injury by suppressing autophagy. Am J Physiol Renal Physiol. 2021 Sep
Subcellular Location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole, Cell projection, cilium, cilium basal body, flagellum.
Synonyms
D13S1056E antibody
DAF19 antibody
hTg737 antibody
Ift88 antibody
IFT88_HUMAN antibody
Intraflagellar transport 88 homolog antibody
Intraflagellar transport protein 88 homolog antibody
MGC26259 antibody
Polaris homolog antibody
Probe hTg737 (polycystic kidney disease, autosomal recessive) antibody
ExpandImages
-
Western blot analysis of IFT88 on different lysates with Rabbit anti-IFT88 antibody (HA723357) at 1/5,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: HeLa cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: C6 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 93 kDa
Observed band size: 93 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723357) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of IFT88 on different lysates with Rabbit anti-IFT88 antibody (HA723357) at 1/2,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: A549 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: C6 cell lysate
Lane 6: COS-1 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 93 kDa
Observed band size: 93 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723357) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-IFT88 antibody (HA723357) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723357) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-IFT88 antibody (HA723357) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723357) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunocytochemistry analysis of NIH/3T3 cells labeling IFT88 with Rabbit anti-IFT88 antibody (HA723357) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IFT88 antibody (HA723357) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of C6 cells labeling IFT88 with Rabbit anti-IFT88 antibody (HA723357) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IFT88 antibody (HA723357) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"