Skip to content

Vasopressin Recombinant Rabbit Monoclonal Antibody [PSH11-64]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# HA723360

Vasopressin Recombinant Rabbit Monoclonal Antibody [PSH11-64]

Application

  • IHC-Fr

  • IHC-P

  • IF-Tissue

Reactivity

  • Mouse

  • Rat

BSA and Azide free
Predicted reactivity

  • Cynomolgus monkey

  • Pig

Predicted species support after-sales service
Conjugation

  • unconjugated

Overview

Product Name

Vasopressin Recombinant Rabbit Monoclonal Antibody [PSH11-64]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Mouse Vasopressin aa 32-124.

Species Reactivity

Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

Predicted species support after-sales service

Validated Applications

IHC-Fr, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 17 kDa

Positive Control

Mouse brain tissue, rat hypothalamus tissue, mouse hypothalamus tissue, mouse hypothalamus (paraventricular third ventricle) tissue, mouse hypothalamus (suprachiasmatic nucleus) tissue.

Conjugation

unconjugated

Clone Number

PSH11-64

Reactivity Data

IHC-Fr IHC-P IF-Tissue
Mouse
Tested Tested
Tested Tested
Tested Tested
Rat
Tested Tested
Cynomolgus Monkey
Published
Published
Published
Pig
Published
Published
Published

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-Fr

  • 1:500

  • IHC-P

  • 1:50,000

  • IF-Tissue

  • 1:1,000

Target

Function

Human vasopressin, also called antidiuretic hormone (ADH), arginine vasopressin (AVP) or argipressin, is a hormone synthesized from the AVP gene as a peptide prohormone in neurons in the hypothalamus, and is converted to AVP. It then travels down the axon terminating in the posterior pituitary, and is released from vesicles into the circulation in response to extracellular fluid hypertonicity (hyperosmolality). AVP has two primary functions. First, it increases the amount of solute-free water reabsorbed back into the circulation from the filtrate in the kidney tubules of the nephrons. Second, AVP constricts arterioles, which increases peripheral vascular resistance and raises arterial blood pressure. A third function is possible. Some AVP may be released directly into the brain from the hypothalamus, and may play an important role in social behavior, sexual motivation and pair bonding, and maternal responses to stress. Vasopressin induces differentiation of stem cells into cardiomyocytes and promotes heart muscle homeostasis. It has a very short half-life, between 16 and 24 minutes.

Background References

1. Rigney N et al. Oxytocin, Vasopressin, and Social Behavior: From Neural Circuits to Clinical Opportunities. Endocrinology. 2022 Sep

2. Fage N et al. Norepinephrine and Vasopressin in Hemorrhagic Shock: A Focus on Renal Hemodynamics. Int J Mol Sci. 2023 Feb

Subcellular Location

Secreted.

UNIPROT

SwissProt: P01185 Human

SwissProt: P35455 Mouse

SwissProt: P01186 Rat

Synonyms

ADH antibody

Antidiuretic hormone antibody

Arginine vasopressin neurophysin II antibody

ARVP antibody

AVP antibody

AVP NPII antibody

copeptin antibody

Vasopressin neurophysin II copeptin antibody

VP antibody

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Hypothalamus <br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:500<br /><br />Antigen retrieval: The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature.

    Application: IHC-Fr

    Species: Mouse

    Site: Hypothalamus

    Sample: Frozen section

    Antibody concentration: 1:500

    Antigen retrieval: The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature.

  • Application: IF-tissue<br /><br />Species: Mouse<br /><br />Site: Hypothalamus <br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1,000

    Application: IF-tissue

    Species: Mouse

    Site: Hypothalamus

    Sample: Paraffin-embedded section

    Antibody concentration: 1,000

  • Application: IF-tissue<br /><br />Species: Mouse<br /><br />Site: Hypothalamus (paraventricular third ventricle)<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1,000

    Application: IF-tissue

    Species: Mouse

    Site: Hypothalamus (paraventricular third ventricle)

    Sample: Paraffin-embedded section

    Antibody concentration: 1,000

  • Application: IF-tissue<br /><br />Species: Mouse<br /><br />Site: Hypothalamus (suprachiasmatic nucleus)<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1,000

    Application: IF-tissue

    Species: Mouse

    Site: Hypothalamus (suprachiasmatic nucleus)

    Sample: Paraffin-embedded section

    Antibody concentration: 1,000

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Vasopressin antibody (<a href="/products/HA723360" style="font-weight: bold;text-decoration: underline;">HA723360</a>) at 1/50,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723360" style="font-weight: bold;text-decoration: underline;">HA723360</a>) at 1/50,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Vasopressin antibody (HA723360) at 1/50,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723360) at 1/50,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Vasopressin antibody (<a href="/products/HA723360" style="font-weight: bold;text-decoration: underline;">HA723360</a>) at 1/50,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723360" style="font-weight: bold;text-decoration: underline;">HA723360</a>) at 1/50,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Vasopressin antibody (HA723360) at 1/50,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723360) at 1/50,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat hypothalamus tissue with Rabbit anti-Vasopressin antibody (<a href="/products/HA723360" style="font-weight: bold;text-decoration: underline;">HA723360</a>) at 1/50,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723360" style="font-weight: bold;text-decoration: underline;">HA723360</a>) at 1/50,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat hypothalamus tissue with Rabbit anti-Vasopressin antibody (HA723360) at 1/50,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723360) at 1/50,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat hypothalamus tissue with Rabbit anti-Vasopressin antibody (<a href="/products/HA723360" style="font-weight: bold;text-decoration: underline;">HA723360</a>) at 1/50,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723360" style="font-weight: bold;text-decoration: underline;">HA723360</a>) at 1/50,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat hypothalamus tissue with Rabbit anti-Vasopressin antibody (HA723360) at 1/50,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723360) at 1/50,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"