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Survivin Recombinant Rabbit Monoclonal Antibody [PSH11-73]

Usd: 385

Specification

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Catalog# HA723369

Survivin Recombinant Rabbit Monoclonal Antibody [PSH11-73]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

Survivin Recombinant Rabbit Monoclonal Antibody [PSH11-73]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human Survivin aa 1-142.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 16 kDa

Positive Control

HeLa cell lysate, HeLa treated with 100ng/mL Nocodazole for 18 hours cell lysate, mouse spleen tissue, mouse testis tissue.

Conjugation

unconjugated

Clone Number

PSH11-73

Reactivity Data

WB IHC-P
Human
Tested Tested
Not Recommended
Mouse
Tested Tested
Tested Tested
Rat
Not Recommended
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

Target

Function

Survivin, also called baculoviral inhibitor of apoptosis repeat-containing 5 or BIRC5, is a protein that, in humans, is encoded by the BIRC5 gene. Survivin is a member of the inhibitor of apoptosis (IAP) family. The survivin protein functions to inhibit caspase activation, thereby leading to negative regulation of apoptosis or programmed cell death. This has been shown by disruption of survivin induction pathways leading to increase in apoptosis and decrease in tumour growth. The survivin protein is expressed highly in most human tumours and fetal tissue, but is completely absent in terminally differentiated cells. These data suggest survivin might provide a new target for cancer therapy that would discriminate between transformed and normal cells. Survivin expression is also highly regulated by the cell cycle and is only expressed in the G2-M phase. It is known that Survivin localizes to the mitotic spindle by interaction with tubulin during mitosis and may play a contributing role in regulating mitosis. The molecular mechanisms of survivin regulation are still not well understood, but regulation of survivin seems to be linked to the p53 protein. It also is a direct target gene of the Wnt pathway and is upregulated by beta-catenin.

Background References

1. Albadari N et al. Survivin Small Molecules Inhibitors: Recent Advances and Challenges. Molecules. 2023 Feb

2. Siragusa G et al. Survivin (BIRC5): Implications in cancer therapy. Life Sci. 2024 Aug

Subcellular Location

Cytoplasm, Nucleus, Chromosome, centromere, cytoskeleton, spindle, kinetochore, Midbody.

UNIPROT

SwissProt: O15392 Human

SwissProt: O70201 Mouse

Synonyms

API4 antibody

Apoptosis inhibitor 4 antibody

Apoptosis inhibitor survivin antibody

Apoptosis inhibitor4 antibody

Baculoviral IAP repeat containing 5 antibody

Baculoviral IAP repeat containing protein 5 antibody

Baculoviral IAP repeat-containing protein 5 antibody

BIRC 5 antibody

BIRC5 antibody

BIRC5_HUMAN antibody

Expand

Images

  • Western blot analysis of Survivin on different lysates with Rabbit anti-Survivin antibody (<a href="/products/HA723369" style="font-weight: bold;text-decoration: underline;">HA723369</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HeLa treated with 100ng/mL Nocodazole for 18 hours cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 16 kDa<br />Observed band size: 16 kDa<br /><br />Exposure time: 12 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723369" style="font-weight: bold;text-decoration: underline;">HA723369</a>) at 1/1,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Survivin on different lysates with Rabbit anti-Survivin antibody (HA723369) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HeLa treated with 100ng/mL Nocodazole for 18 hours cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 16 kDa
    Observed band size: 16 kDa

    Exposure time: 12 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723369) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-Survivin antibody (<a href="/products/HA723369" style="font-weight: bold;text-decoration: underline;">HA723369</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723369" style="font-weight: bold;text-decoration: underline;">HA723369</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-Survivin antibody (HA723369) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723369) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Survivin antibody (<a href="/products/HA723369" style="font-weight: bold;text-decoration: underline;">HA723369</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723369" style="font-weight: bold;text-decoration: underline;">HA723369</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Survivin antibody (HA723369) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723369) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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