HRP Conjugated Human sTNF RI Recombinant Rabbit Monoclonal Antibody [PSH09-90] - Detector
Overview
Product Name
HRP Conjugated Human sTNF RI Recombinant Rabbit Monoclonal Antibody [PSH09-90] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human TNF RI aa 30-211.
Species Reactivity
Human
Validated Applications
ELISA(Det)
Positive Control
Recombinant Human sTNF RI Protein (HA210562).
Conjugation
HRP
Clone Number
PSH09-90
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH09-89] to Human sTNF RI antibody (Capture) (HA723158) and Recombinant Human sTNF RI protein (HA210562) as the standard.
Target
Function
This gene encodes a member of the TNF receptor superfamily of proteins. The encoded receptor is found in membrane-bound and soluble forms that interact with membrane-bound and soluble forms, respectively, of its ligand, tumor necrosis factor alpha. Binding of membrane-bound tumor necrosis factor alpha to the membrane-bound receptor induces receptor trimerization and activation, which plays a role in cell survival, apoptosis, and inflammation. Proteolytic processing of the encoded receptor results in release of the soluble form of the receptor, which can interact with free tumor necrosis factor alpha to inhibit inflammation. Mutations in this gene underlie tumor necrosis factor receptor-associated periodic syndrome (TRAPS), characterized by fever, abdominal pain and other features. Mutations in this gene may also be associated with multiple sclerosis in human patients.
Background References
1. Nophar Y., Kemper O., Brakebusch C., Engelmann H., Zwang R., Aderka D., Holtmann H., Wallach D. Soluble forms of tumor necrosis factor receptors (TNF-Rs). The cDNA for the type I TNF-R, cloned using amino acid sequence data of its soluble form, encodes both the cell surface and a soluble form of the receptor. EMBO J. 9:3269-3278 (1990)
2. Gregory A.P., Dendrou C.A., Attfield K.E., Haghikia A., Xifara D.K., Butter F., Poschmann G., Kaur G., Lambert L., Fugger L. TNF receptor 1 genetic risk mirrors outcome of anti-TNF therapy in multiple sclerosis. Nature 488:508-511 (2012)
Subcellular Location
Secreted. Cell membrane.
UNIPROT
Synonyms
CD120a antibody
FPF antibody
MGC19588 antibody
p55 antibody
p55-R antibody
p60 antibody
TBP1 antibody
TBPI antibody
TNF R antibody
TNF R55 antibody
ExpandImages
-
Sandwich ELISA analysis of Human sTNF RI matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723154) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human sTNF RI Protein (HA210562) starting from 50 ng/ml to 0 pg/ml and detect antibody (HA723374H, HRP, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"