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BAF170 Recombinant Rabbit Monoclonal Antibody [PSH12-00]

Usd: 385

Specification

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Catalog# HA723413

BAF170 Recombinant Rabbit Monoclonal Antibody [PSH12-00]

Application

  • WB

  • IHC-P

  • IF-Cell

  • IP

  • ChIP

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

BAF170 Recombinant Rabbit Monoclonal Antibody [PSH12-00]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human BAF170 aa 915-1,214.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Cell, IP, ChIP

Molecular Weight

Predicted band size: 133 kDa

Positive Control

K-562 cell lysate, A431 cell lysate, MCF7 cell lysate, HeLa cell lysate, SW620 cell lysate, SW680 cell lysate, PANC cell lysate, C2C12 cell lysate, NIH/3T3 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, human brain tissue, human stomach tissue, mouse brain tissue, mouse stomach tissue, rat brain tissue, rat stomach tissue, HeLa, Neuro-2a.

Conjugation

unconjugated

Clone Number

PSH12-00

Reactivity Data

WB IHC-P IF-Cell IP ChIP
Human
Tested Tested
Tested Tested
Tested Tested
Tested Tested
Tested Tested
Mouse
Tested Tested
Tested Tested
Tested Tested
Rat
Tested Tested
Tested Tested
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:1,000

  • IF-Cell

  • 1:500

  • IP

  • 1-2μg/sample

  • ChIP

  • Use 5 μg for 25 μg of chromatin.

Target

Function

SMARCC2 (BAF170) is a core subunit of the SWI/SNF chromatin remodeling complex, which regulates gene expression by altering chromatin structure in an ATP-dependent manner. This complex is essential for transcription, DNA replication, and repair, and plays a key role in cell growth, differentiation, and development. SMARCC2/BAF170 enhances the ATPase activity of the catalytic subunit BRG1, facilitating nucleosome remodeling and gene regulation. During neural development, SMARCC2/BAF170 is part of both the neural progenitor-specific (npBAF) and neuron-specific (nBAF) chromatin remodeling complexes. The npBAF complex supports neural stem cell self-renewal, while the nBAF complex regulates dendrite growth and neuronal gene expression. SMARCC2/BAF170 also plays a critical role in myeloid differentiation, particularly in granulocytopoiesis and neutrophil granule formation. Its expression is tightly regulated, and its upregulation is associated with the loss of pluripotency in embryonic stem cells.

Background References

1. Bosch E et al. Elucidating the clinical and molecular spectrum of SMARCC2-associated NDD in a cohort of 65 affected individuals. Genet Med. 2023 Nov

2. Sun Z et al. SMARCC2 silencing suppresses oncogenic activation through modulation of chromatin accessibility in breast cancer. Biochem Biophys Res Commun. 2024 Sep

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q8TAQ2 Human

SwissProt: Q6PDG5 Mouse

Entrez Gene: 362815 Rat

Synonyms

BAF 170 antibody

BAF170 antibody

BRG1 associated factor 170 antibody

BRG1-associated factor 170 antibody

Chromatin remodeling complex BAF170 subunit antibody

CRACC 2 antibody

CRACC2 antibody

Mammalian chromatin remodeling complex BRG1 associated factor 170 antibody

Rsc 8 antibody

Rsc8 antibody

Expand

Images

  • Western blot analysis of BAF170 on different lysates with Rabbit anti-BAF170 antibody (<a href="/products/HA723413" style="font-weight: bold;text-decoration: underline;">HA723413</a>) at 1/5,000 dilution.<br /><br />Lane 1: K-562 cell lysate (20 µg/Lane)<br />Lane 2: A431 cell lysate (20 µg/Lane)<br />Lane 3: MCF7 cell lysate (20 µg/Lane)<br />Lane 4: HeLa cell lysate (20 µg/Lane)<br />Lane 5: SW620 cell lysate (20 µg/Lane)<br />Lane 6: SW680 cell lysate (20 µg/Lane)<br />Lane 7: PANC cell lysate (20 µg/Lane)<br />Lane 8: C2C12 cell lysate (20 µg/Lane)<br />Lane 9: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 10: Neuro-2a cell lysate (20 µg/Lane)<br />Lane 11: PC-12 cell lysate (20 µg/Lane)<br />Lane 12: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 13: Rat brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 133 kDa<br />Observed band size: 162 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723413" style="font-weight: bold;text-decoration: underline;">HA723413</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of BAF170 on different lysates with Rabbit anti-BAF170 antibody (HA723413) at 1/5,000 dilution.

    Lane 1: K-562 cell lysate (20 µg/Lane)
    Lane 2: A431 cell lysate (20 µg/Lane)
    Lane 3: MCF7 cell lysate (20 µg/Lane)
    Lane 4: HeLa cell lysate (20 µg/Lane)
    Lane 5: SW620 cell lysate (20 µg/Lane)
    Lane 6: SW680 cell lysate (20 µg/Lane)
    Lane 7: PANC cell lysate (20 µg/Lane)
    Lane 8: C2C12 cell lysate (20 µg/Lane)
    Lane 9: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 10: Neuro-2a cell lysate (20 µg/Lane)
    Lane 11: PC-12 cell lysate (20 µg/Lane)
    Lane 12: Mouse brain tissue lysate (40 µg/Lane)
    Lane 13: Rat brain tissue lysate (40 µg/Lane)

    Predicted band size: 133 kDa
    Observed band size: 162 kDa

    Exposure time: 25 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723413) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-BAF170 antibody (<a href="/products/HA723413" style="font-weight: bold;text-decoration: underline;">HA723413</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723413" style="font-weight: bold;text-decoration: underline;">HA723413</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-BAF170 antibody (HA723413) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723413) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-BAF170 antibody (<a href="/products/HA723413" style="font-weight: bold;text-decoration: underline;">HA723413</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723413" style="font-weight: bold;text-decoration: underline;">HA723413</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-BAF170 antibody (HA723413) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723413) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-BAF170 antibody (<a href="/products/HA723413" style="font-weight: bold;text-decoration: underline;">HA723413</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723413" style="font-weight: bold;text-decoration: underline;">HA723413</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-BAF170 antibody (HA723413) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723413) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-BAF170 antibody (<a href="/products/HA723413" style="font-weight: bold;text-decoration: underline;">HA723413</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723413" style="font-weight: bold;text-decoration: underline;">HA723413</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-BAF170 antibody (HA723413) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723413) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-BAF170 antibody (<a href="/products/HA723413" style="font-weight: bold;text-decoration: underline;">HA723413</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723413" style="font-weight: bold;text-decoration: underline;">HA723413</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-BAF170 antibody (HA723413) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723413) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat stomach tissue with Rabbit anti-BAF170 antibody (<a href="/products/HA723413" style="font-weight: bold;text-decoration: underline;">HA723413</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723413" style="font-weight: bold;text-decoration: underline;">HA723413</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue with Rabbit anti-BAF170 antibody (HA723413) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723413) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunocytochemistry analysis of HeLa cells labeling BAF170 with Rabbit anti-BAF170 antibody (<a href="/products/HA723413" style="font-weight: bold;text-decoration: underline;">HA723413</a>) at 1/500 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BAF170 antibody (<a href="/products/HA723413" style="font-weight: bold;text-decoration: underline;">HA723413</a>) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HeLa cells labeling BAF170 with Rabbit anti-BAF170 antibody (HA723413) at 1/500 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BAF170 antibody (HA723413) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunocytochemistry analysis of Neuro-2a cells labeling BAF170 with Rabbit anti-BAF170 antibody (<a href="/products/HA723413" style="font-weight: bold;text-decoration: underline;">HA723413</a>) at 1/500 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BAF170 antibody (<a href="/products/HA723413" style="font-weight: bold;text-decoration: underline;">HA723413</a>) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of Neuro-2a cells labeling BAF170 with Rabbit anti-BAF170 antibody (HA723413) at 1/500 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BAF170 antibody (HA723413) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • BAF170 was immunoprecipitated from 0.2 mg MCF7 cell lysate with <a href="/products/HA723413" style="font-weight: bold;text-decoration: underline;">HA723413</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA723413" style="font-weight: bold;text-decoration: underline;">HA723413</a> at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: MCF7 cell lysate (input)<br />Lane 2: <a href="/products/HA723413" style="font-weight: bold;text-decoration: underline;">HA723413</a> IP in MCF7 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA723413" style="font-weight: bold;text-decoration: underline;">HA723413</a> in MCF7 cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 30 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    BAF170 was immunoprecipitated from 0.2 mg MCF7 cell lysate with HA723413 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723413 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: MCF7 cell lysate (input)
    Lane 2: HA723413 IP in MCF7 cell lysate
    Lane 3: Rabbit IgG instead of HA723413 in MCF7 cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 30 seconds; ECL: K1801

  • Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells with BAF170 (<a href="/products/HA723413" style="font-weight: bold;text-decoration: underline;">HA723413</a>) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

    Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells with BAF170 (HA723413) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

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