Mouse IL-18 Recombinant Rabbit Monoclonal Antibody [PSH12-04] - BSA and Azide free (Detector)
Overview
Product Name
Mouse IL-18 Recombinant Rabbit Monoclonal Antibody [PSH12-04] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mouse IL-18 aa 36-192 (HA210940).
Species Reactivity
Mouse
Validated Applications
ELISA(Det)
Positive Control
Recombinant Mouse IL-18 protein (HA210940).
Conjugation
unconjugated
Clone Number
PSH12-04
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-03] to Mouse IL-18 antibody (Capture) (HA723416) and recombinant Mouse IL-18 protein (HA210940) as the standard. The reference range value is 31.3-8,000 pg/mL.
Target
Function
Interleukin-18 (IL-18), also known as interferon-gamma inducing factor is a protein which in humans is encoded by the IL18 gene. The protein encoded by this gene is a proinflammatory cytokine. Many cell types, both hematopoietic cells and non-hematopoietic cells, have the potential to produce IL-18. It was first described in 1989 as a factor that induced interferon-γ (IFN-γ) production in mouse spleen cells. Originally, IL-18 production was recognized in Kupffer cells, liver-resident macrophages. However, IL-18 is constitutively expressed in non-hematopoietic cells, such as intestinal epithelial cells, keratinocytes, and endothelial cells. IL-18 can modulate both innate and adaptive immunity and its dysregulation can cause autoimmune or inflammatory diseases. IL-18 belongs to the IL-1 superfamily and is produced mainly by macrophages but also other cell types, stimulates various cell types and has pleiotropic functions. IL-18 is a proinflammatory cytokine that facilitates type 1 responses. Together with IL-12, it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). IL-18 in combination with IL12 acts on CD4, CD8 T cells and NK cells to induce IFNγ production, type II interferon that plays an important role in activating the macrophages or other cells. The combination of this IL-18 and IL-12 has been shown to inhibit IL-4 dependent IgE and IgG1 production and enhance IgG2a production in B cells. Importantly, without IL-12 or IL-15, IL-18 does not induce IFNγ production, but plays an important role in the differentiation of naive T cells into Th2 cells and stimulates mast cells and basophils to produce IL-4, IL-13, and chemical mediators such as histamine.
Background References
1. Zhang X et al. IL18 signaling causes islet β cell development and insulin secretion via different receptors on acinar and β cells. Dev Cell. 2022 Jun
2. Lin T et al. NET-Triggered NLRP3 Activation and IL18 Release Drive Oxaliplatin-Induced Peripheral Neuropathy. Cancer Immunol Res. 2022 Dec
Subcellular Location
Cytoplasm, Secreted.
UNIPROT
Synonyms
Iboctadekin antibody
IFN gamma inducing factor antibody
IFN-gamma-inducing factor antibody
IGIF antibody
IL 1 gamma antibody
IL 18 antibody
IL 1g antibody
IL-1 gamma antibody
IL-18 antibody
IL1 gamma antibody
ExpandImages
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![Sandwich ELISA analysis of Mouse IL-18 matched pair antibodies<br /><br />Capture: <a href="/products/HA723416" style="font-weight: bold;text-decoration: underline;">HA723416</a>, Mouse IL-18 Rabbit mAb [PSH12-03]<br />Detector: <a href="/products/HA723417" style="font-weight: bold;text-decoration: underline;">HA723417</a>, Mouse IL-18 Rabbit mAb [PSH12-04]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (<a href="/products/HA723416" style="font-weight: bold;text-decoration: underline;">HA723416</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-18 protein (<a href="/products/HA210940" style="font-weight: bold;text-decoration: underline;">HA210940</a>) starting from 8,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723417" style="font-weight: bold;text-decoration: underline;">HA723417</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723417_1.png?v=20260429161927)
Sandwich ELISA analysis of Mouse IL-18 matched pair antibodies
Capture: HA723416, Mouse IL-18 Rabbit mAb [PSH12-03]
Detector: HA723417, Mouse IL-18 Rabbit mAb [PSH12-04]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723416) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-18 protein (HA210940) starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA723417, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native IL-18 in mouse spleen and Neuro-2a extract samples based on a 1000 µg/ml extract load.<br /><br />Capture: <a href="/products/HA723416" style="font-weight: bold;text-decoration: underline;">HA723416</a>, Mouse IL-18 Rabbit mAb [PSH12-03]<br />Detector: <a href="/products/HA723956" style="font-weight: bold;text-decoration: underline;">HA723956</a>, Mouse IL-18 Rabbit mAb [PSH12-04]<br /><br />Interpolated concentration of native IL-18 was measured in duplicate at different sample concentrations and interpolated from the IL-18 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean IL-18 concentration was determined to be 37,776 pg/mL in mouse spleen tissue extract, There was no detectable signal in Neuro-2a cell extract.](https://storage.huabio.cn/huabio/productImg/HA723417_2.png?v=20260429161927)
Interpolated concentrations of native IL-18 in mouse spleen and Neuro-2a extract samples based on a 1000 µg/ml extract load.
Capture: HA723416, Mouse IL-18 Rabbit mAb [PSH12-03]
Detector: HA723956, Mouse IL-18 Rabbit mAb [PSH12-04]
Interpolated concentration of native IL-18 was measured in duplicate at different sample concentrations and interpolated from the IL-18 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean IL-18 concentration was determined to be 37,776 pg/mL in mouse spleen tissue extract, There was no detectable signal in Neuro-2a cell extract. -
![Interpolated concentrations of spiked IL-18 in cell culture media samples.<br /><br />Capture: <a href="/products/HA723416" style="font-weight: bold;text-decoration: underline;">HA723416</a>, Mouse IL-18 Rabbit mAb [PSH12-03]<br />Detector: <a href="/products/HA723956" style="font-weight: bold;text-decoration: underline;">HA723956</a>, Mouse IL-18 Rabbit mAb [PSH12-04]<br /><br />The concentrations of IL-18 were measured in duplicates, interpolated from the IL-18 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).](https://storage.huabio.cn/huabio/productImg/HA723417_3.png?v=20260429161927)
Interpolated concentrations of spiked IL-18 in cell culture media samples.
Capture: HA723416, Mouse IL-18 Rabbit mAb [PSH12-03]
Detector: HA723956, Mouse IL-18 Rabbit mAb [PSH12-04]
The concentrations of IL-18 were measured in duplicates, interpolated from the IL-18 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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