TFEB Recombinant Rabbit Monoclonal Antibody [PSH12-05]
Catalog# HA723419
TFEB Recombinant Rabbit Monoclonal Antibody [PSH12-05]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
TFEB Recombinant Rabbit Monoclonal Antibody [PSH12-05]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human TFEB aa 301-476.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 53 kDa
Positive Control
Raji cell lysate, HeLa cell lysate, RAW264.7 cell lysate, Mouse colon tissue lysate, Rat colon tissue lysate.
Conjugation
unconjugated
Clone Number
PSH12-05
Reactivity Data
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:5,000
Target
Function
Transcription factor EB is a protein that in humans is encoded by the TFEB gene. TFEB is a master gene for lysosomal biogenesis. It encodes a transcription factor that coordinates expression of lysosomal hydrolases, membrane proteins and genes involved in autophagy. Upon nutrient depletion and under aberrant lysosomal storage conditions such as in lysosomal storage diseases, TFEB translocates from the cytoplasm to the nucleus, resulting in the activation of its target genes. TFEB overexpression in cultured cells induces lysosomal biogenesis, exocytosis and autophagy.
Background References
1. Franco-Juárez B et al. TFEB; Beyond Its Role as an Autophagy and Lysosomes Regulator. Cells. 2022 Oct
2. Cui Z et al. Structure of the lysosomal mTORC1-TFEB-Rag-Ragulator megacomplex. Nature. 2023 Feb
Subcellular Location
Nucleus, Cytoplasm, cytosol, Lysosome membrane.
Synonyms
Alpha TFEB antibody
AlphaTFEB antibody
bHLHe35 antibody
Class E basic helix-loop-helix protein 35 antibody
T cell transcription factor EB antibody
TCFEB antibody
TFEB antibody
TFEB_HUMAN antibody
Transcription factor EB antibody
Images
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Western blot analysis of TFEB on different lysates with Rabbit anti-TFEB antibody (HA723419) at 1/5,000 dilution.
Lane 1: Raji cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: RAW264.7 cell lysate (20 µg/Lane)
Lane 4: Mouse colon tissue lysate (40 µg/Lane)
Lane 5: Rat colon tissue lysate (40 µg/Lane)
Predicted band size: 53 kDa
Observed band size: 70 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723419) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"