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TFEB Recombinant Rabbit Monoclonal Antibody [PSH12-05]

Usd: 385

Specification

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Catalog# HA723419

TFEB Recombinant Rabbit Monoclonal Antibody [PSH12-05]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

TFEB Recombinant Rabbit Monoclonal Antibody [PSH12-05]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human TFEB aa 301-476.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 53 kDa

Positive Control

Raji cell lysate, HeLa cell lysate, RAW264.7 cell lysate, Mouse colon tissue lysate, Rat colon tissue lysate.

Conjugation

unconjugated

Clone Number

PSH12-05

Reactivity Data

WB
Human
Tested Tested
Mouse
Tested Tested
Rat
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

Target

Function

Transcription factor EB is a protein that in humans is encoded by the TFEB gene. TFEB is a master gene for lysosomal biogenesis. It encodes a transcription factor that coordinates expression of lysosomal hydrolases, membrane proteins and genes involved in autophagy. Upon nutrient depletion and under aberrant lysosomal storage conditions such as in lysosomal storage diseases, TFEB translocates from the cytoplasm to the nucleus, resulting in the activation of its target genes. TFEB overexpression in cultured cells induces lysosomal biogenesis, exocytosis and autophagy.

Background References

1. Franco-Juárez B et al. TFEB; Beyond Its Role as an Autophagy and Lysosomes Regulator. Cells. 2022 Oct

2. Cui Z et al. Structure of the lysosomal mTORC1-TFEB-Rag-Ragulator megacomplex. Nature. 2023 Feb

Subcellular Location

Nucleus, Cytoplasm, cytosol, Lysosome membrane.

UNIPROT

SwissProt: P19484 Human

SwissProt: Q9R210 Mouse

Entrez Gene: 316214 Rat

Synonyms

Alpha TFEB antibody

AlphaTFEB antibody

bHLHe35 antibody

Class E basic helix-loop-helix protein 35 antibody

T cell transcription factor EB antibody

TCFEB antibody

TFEB antibody

TFEB_HUMAN antibody

Transcription factor EB antibody

Images

  • Western blot analysis of TFEB on different lysates with Rabbit anti-TFEB antibody (<a href="/products/HA723419" style="font-weight: bold;text-decoration: underline;">HA723419</a>) at 1/5,000 dilution.<br /><br />Lane 1: Raji cell lysate (20 µg/Lane)<br />Lane 2: HeLa cell lysate (20 µg/Lane)<br />Lane 3: RAW264.7 cell lysate (20 µg/Lane)<br />Lane 4: Mouse colon tissue lysate (40 µg/Lane)<br />Lane 5: Rat colon tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 53 kDa<br />Observed band size: 70 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723419" style="font-weight: bold;text-decoration: underline;">HA723419</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TFEB on different lysates with Rabbit anti-TFEB antibody (HA723419) at 1/5,000 dilution.

    Lane 1: Raji cell lysate (20 µg/Lane)
    Lane 2: HeLa cell lysate (20 µg/Lane)
    Lane 3: RAW264.7 cell lysate (20 µg/Lane)
    Lane 4: Mouse colon tissue lysate (40 µg/Lane)
    Lane 5: Rat colon tissue lysate (40 µg/Lane)

    Predicted band size: 53 kDa
    Observed band size: 70 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723419) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"