Biotin Conjugated Mouse/Rat Factor XII Recombinant Rabbit Monoclonal Antibody [PSH12-23] - Detector
Overview
Product Name
Biotin Conjugated Mouse/Rat Factor XII Recombinant Rabbit Monoclonal Antibody [PSH12-23] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mouse Factor XII aa 20-597 (HA210984).
Species Reactivity
Mouse, Rat
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Mouse Factor XII protein (HA210984).
Conjugation
Biotin
Clone Number
PSH12-23
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-21] to Mouse/Rat Factor XII antibody (Capture) (HA723443) and Recombinant Mouse Factor XII protein (HA210984) as the standard. The reference range value is 469-30,000 pg/mL.
-
ELISA
-
Use at an assay dependent concentration.
Target
Function
Coagulation factor XII, also known as Hageman factor, is a plasma protein involved in coagulation. It is the zymogen form of factor XIIa (EC 3.4.21.38), an enzyme of the serine protease (or serine endopeptidase) class. In humans, factor XII is encoded by F12 gene. Factor XII (FXII, Hageman factor) is a plasma glycoprotein of approximately 90 kDa and is part of the coagulation cascade. It activates factor XI and prekallikrein in vitro. FXII is activated to FXIIa by negatively charged surfaces such as glass, initiating the intrinsic pathway. FXII also plays a role in diagnostic coagulation assays called activated partial thromboplastin times (aPTT). In vivo, FXII is activated by binding to polyanions in a process termed contact activation. Various polymers, such as kaolin and glass, act as non-physiological activators of FXII. Activated platelets release inorganic polyphosphates, which activate FXII, initiating the intrinsic pathway and contributing to fibrin formation and thrombus development. Polyphosphates also accelerate thrombin-induced activation of factor XI. Polyphosphate forms calcium-rich nanoparticles in vivo, which accumulate on platelets and activate FXII. The regulation of platelet polyphosphates is still not fully understood, but the phosphate-exporter XPR1 has been identified as a key regulator. Targeting XPR1 increases polyphosphate levels, accelerating thrombosis in mouse models. Given FXII’s role in thrombosis while sparing hemostasis, it has become a target for anticoagulant drug development. Inhibitors targeting FXII are in clinical trials.
Background References
1. Shamanaev A et al. Factor XII Structure-Function Relationships. Semin Thromb Hemost. 2024 Oct
2. Sparkenbaugh EM et al. Factor XII contributes to thrombotic complications and vaso-occlusion in sickle cell disease. Blood. 2023 Apr
Subcellular Location
Secreted.
Synonyms
Factor XII antibody
Beta factor XIIa part 1 antibody
Beta factor XIIa part 2 antibody
Coagulation factor XII antibody
Coagulation factor XIIa heavy chain antibody
Coagulation factor XIIa light chain antibody
F12 antibody
F12 deficiency antibody
FA12_HUMAN antibody
Factor XII deficiency antibody
ExpandImages
-
![Sandwich ELISA analysis of Mouse Factor XII matched pair antibodies<br /><br />Capture: <a href="/products/HA723443" style="font-weight: bold;text-decoration: underline;">HA723443</a>, Mouse/Rat Factor XII Rabbit mAb [PSH12-21]<br />Detector: <a href="/products/HA723444" style="font-weight: bold;text-decoration: underline;">HA723444</a>, Mouse/Rat Factor XII Rabbit mAb [PSH12-23]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA723443" style="font-weight: bold;text-decoration: underline;">HA723443</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Factor XII protein (<a href="/products/HA210984" style="font-weight: bold;text-decoration: underline;">HA210984</a>) starting from 18,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723444" style="font-weight: bold;text-decoration: underline;">HA723444</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723445B_1.png?v=20260604174823)
Sandwich ELISA analysis of Mouse Factor XII matched pair antibodies
Capture: HA723443, Mouse/Rat Factor XII Rabbit mAb [PSH12-21]
Detector: HA723444, Mouse/Rat Factor XII Rabbit mAb [PSH12-23]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723443) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Factor XII protein (HA210984) starting from 18,000 pg/ml to 0 pg/ml and detect antibody (HA723444, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native Factor XII in mouse samples.<br /><br />Capture: <a href="/products/HA723443" style="font-weight: bold;text-decoration: underline;">HA723443</a>, Mouse/Rat Factor XII Rabbit mAb [PSH12-21]<br />Detector: <a href="/products/HA723444" style="font-weight: bold;text-decoration: underline;">HA723444</a>, Mouse/Rat Factor XII Rabbit mAb [PSH12-23]<br /><br />Interpolated concentration of native Factor XII was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Factor XII concentration was determined to be 22,555,940 pg/mL in mouse serum and 20,935 pg/ml in mouse liver Supernatant.](https://storage.huabio.cn/huabio/productImg/HA723445B_2.png?v=20260604174823)
Interpolated concentrations of native Factor XII in mouse samples.
Capture: HA723443, Mouse/Rat Factor XII Rabbit mAb [PSH12-21]
Detector: HA723444, Mouse/Rat Factor XII Rabbit mAb [PSH12-23]
Interpolated concentration of native Factor XII was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Factor XII concentration was determined to be 22,555,940 pg/mL in mouse serum and 20,935 pg/ml in mouse liver Supernatant. -
![Interpolated concentrations of spiked Factor XII in cell culture media samples.<br /><br />Capture: <a href="/products/HA723443" style="font-weight: bold;text-decoration: underline;">HA723443</a>, Mouse/Rat Factor XII Rabbit mAb [PSH12-21]<br />Detector: <a href="/products/HA723444" style="font-weight: bold;text-decoration: underline;">HA723444</a>, Mouse/Rat Factor XII Rabbit mAb [PSH12-23]<br /><br />The concentrations of Factor XII were measured in duplicates, interpolated from the Factor XII standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).](https://storage.huabio.cn/huabio/productImg/HA723445B_3.png?v=20260604174823)
Interpolated concentrations of spiked Factor XII in cell culture media samples.
Capture: HA723443, Mouse/Rat Factor XII Rabbit mAb [PSH12-21]
Detector: HA723444, Mouse/Rat Factor XII Rabbit mAb [PSH12-23]
The concentrations of Factor XII were measured in duplicates, interpolated from the Factor XII standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). -
![Interpolated concentrations of native Factor XII in rat samples.<br /><br />Capture: <a href="/products/HA723443" style="font-weight: bold;text-decoration: underline;">HA723443</a>, Mouse/Rat Factor XII Rabbit mAb [PSH12-21]<br />Detector: <a href="/products/HA723444" style="font-weight: bold;text-decoration: underline;">HA723444</a>, Mouse/Rat Factor XII Rabbit mAb [PSH12-23]<br /><br />Interpolated concentration of native Factor XII was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Factor XII concentration was determined to be 16,655,930 pg/mL in rat serum.](https://storage.huabio.cn/huabio/productImg/HA723445B_4.png?v=20260604174823)
Interpolated concentrations of native Factor XII in rat samples.
Capture: HA723443, Mouse/Rat Factor XII Rabbit mAb [PSH12-21]
Detector: HA723444, Mouse/Rat Factor XII Rabbit mAb [PSH12-23]
Interpolated concentration of native Factor XII was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Factor XII concentration was determined to be 16,655,930 pg/mL in rat serum.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"