Biotin Conjugated Mouse CD68 Recombinant Rabbit Monoclonal Antibody [PSH12-27] - Detector
Overview
Product Name
Biotin Conjugated Mouse CD68 Recombinant Rabbit Monoclonal Antibody [PSH12-27] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mouse CD68 aa 21-291 (HA211131).
Species Reactivity
Mouse
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Mouse CD68 protein (HA211131).
Conjugation
Biotin
Clone Number
PSH12-27
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
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Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-26] to Mouse CD68 antibody (Capture) (HA723449) and Recombinant Mouse CD68 protein (HA211131) as the standard. The reference range value is 31.3-4,000 pg/ml.
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ELISA
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Use at an assay dependent concentration.
Target
Function
CD68 (Cluster of Differentiation 68) is a protein highly expressed by cells in the monocyte lineage (e.g., monocytic phagocytes, osteoclasts), by circulating macrophages, and by tissue macrophages (e.g., Kupffer cells, microglia). Human CD68 is a Type I transmembrane glycoprotein, heavily glycosylated in its extracellular domain, with a molecular weight of 110 kD. Its primary sequence consists of 354 amino acids with predicted molecular weight of 37.4 kD if it were not glycosylated. The human CD68 protein is encoded by the "CD68" gene which maps to Chromosome 17. Other names or aliases for this gene in humans and other animals include: CD68 Molecule, CD68 Antigen, GP110, Macrosialin, Scavenger Receptor Class D, Member 1, SCARD1, and LAMP4. The mouse equivalent is known as "macrosialin".
Background References
1. Deng R et al. Periosteal CD68(+) F4/80(+) Macrophages Are Mechanosensitive for Cortical Bone Formation by Secretion and Activation of TGF-beta1. Adv Sci (Weinh). 2022 Jan
2. Deng G et al. SPI1(+)CD68(+) macrophages as a biomarker for gastric cancer metastasis: a rationale for combined antiangiogenic and immunotherapy strategies. J Immunother Cancer. 2024 Oct
Subcellular Location
Endosome membrane, Lysosome membrane; Cell membrane.
UNIPROT
Synonyms
CD 68 antibody
CD68 antibody
CD68 antigen antibody
CD68 molecule antibody
CD68_HUMAN antibody
DKFZp686M18236 antibody
gp11 antibody
Gp110 antibody
LAMP4 antibody
Macrophage antigen CD68 (microsialin) antibody
ExpandImages
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![Sandwich ELISA analysis of Mouse CD68 matched pair antibodies<br /><br />Capture: <a href="/products/HA723449" style="font-weight: bold;text-decoration: underline;">HA723449</a>, Mouse CD68 Rabbit mAb [PSH12-26]<br />Detector: <a href="/products/HA723450" style="font-weight: bold;text-decoration: underline;">HA723450</a>, Mouse CD68 Rabbit mAb [PSH12-27]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA723449" style="font-weight: bold;text-decoration: underline;">HA723449</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CD68 protein (<a href="/products/HA211131" style="font-weight: bold;text-decoration: underline;">HA211131</a>) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723450" style="font-weight: bold;text-decoration: underline;">HA723450</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723451B_1.png?v=20260604174822)
Sandwich ELISA analysis of Mouse CD68 matched pair antibodies
Capture: HA723449, Mouse CD68 Rabbit mAb [PSH12-26]
Detector: HA723450, Mouse CD68 Rabbit mAb [PSH12-27]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723449) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CD68 protein (HA211131) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723450, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native CD68 in mouse spleen, Raw264.7 and Neuro-2a extract samples based on a 1000 µg/ml extract load.<br /><br />Capture: <a href="/products/HA723449" style="font-weight: bold;text-decoration: underline;">HA723449</a>, Mouse CD68 Rabbit mAb [PSH12-26]<br />Detector: <a href="/products/HA723450" style="font-weight: bold;text-decoration: underline;">HA723450</a>, Mouse CD68 Rabbit mAb [PSH12-27]<br /><br />Interpolated concentration of native CD68 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD68 concentration was determined to be 35353 pg/mL in mouse spleen extract,47421 pg/ml in Raw264.7 extract and undetectable in Neuro-2a extract.](https://storage.huabio.cn/huabio/productImg/HA723451B_2.png?v=20260604174822)
Interpolated concentrations of native CD68 in mouse spleen, Raw264.7 and Neuro-2a extract samples based on a 1000 µg/ml extract load.
Capture: HA723449, Mouse CD68 Rabbit mAb [PSH12-26]
Detector: HA723450, Mouse CD68 Rabbit mAb [PSH12-27]
Interpolated concentration of native CD68 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD68 concentration was determined to be 35353 pg/mL in mouse spleen extract,47421 pg/ml in Raw264.7 extract and undetectable in Neuro-2a extract. -
![Interpolated concentrations of spiked CD68 in cell culture media samples.<br /><br />Capture: <a href="/products/HA723449" style="font-weight: bold;text-decoration: underline;">HA723449</a>, Mouse CD68 Rabbit mAb [PSH12-26]<br />Detector: <a href="/products/HA723450" style="font-weight: bold;text-decoration: underline;">HA723450</a>, Mouse CD68 Rabbit mAb [PSH12-27]<br /><br />The concentrations of CD68 were measured in duplicates, interpolated from the CD68 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).](https://storage.huabio.cn/huabio/productImg/HA723451B_3.png?v=20260604174822)
Interpolated concentrations of spiked CD68 in cell culture media samples.
Capture: HA723449, Mouse CD68 Rabbit mAb [PSH12-26]
Detector: HA723450, Mouse CD68 Rabbit mAb [PSH12-27]
The concentrations of CD68 were measured in duplicates, interpolated from the CD68 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"