Biotin Conjugated Human Oxytocin-neurophysin 1 Recombinant Rabbit Monoclonal Antibody [PSH12-29] - Detector
Overview
Product Name
Biotin Conjugated Human Oxytocin-neurophysin 1 Recombinant Rabbit Monoclonal Antibody [PSH12-29] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Oxytocin-neurophysin 1 aa 20-125 (HA211134).
Species Reactivity
Human
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Human Oxytocin-neurophysin 1 protein (HA211134).
Conjugation
Biotin
Clone Number
PSH12-29
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-28] to Human Oxytocin-neurophysin 1 antibody (Capture) (HA723452) and Recombinant Human Oxytocin-neurophysin 1 protein (HA211134) as the standard. The reference range value is 78.1-10,000 pg/mL.
-
ELISA
-
Use at an assay dependent concentration.
Target
Function
Oxytocin is a peptide hormone and neuropeptide normally produced in the hypothalamus and released by the posterior pituitary. Present in animals since early stages of evolution, in humans it plays roles in behavior that include social bonding, love, reproduction, childbirth, and the period after childbirth. Oxytocin is released into the bloodstream as a hormone in response to sexual activity and during childbirth. It is also available in pharmaceutical form. In either form, oxytocin stimulates uterine contractions to speed up the process of childbirth. In its natural form, it also plays a role in maternal bonding and milk production. Production and secretion of oxytocin is controlled by a positive feedback mechanism, where its initial release stimulates production and release of further oxytocin. For example, when oxytocin is released during a contraction of the uterus at the start of childbirth, this stimulates production and release of more oxytocin and an increase in the intensity and frequency of contractions. This process compounds in intensity and frequency and continues until the triggering activity ceases. A similar process takes place during lactation and during sexual activity.
Background References
1. De Leon D et al. Methylation of OXT and OXTR genes, central oxytocin, and social behavior in female macaques. Horm Behav. 2020 Nov
2. Khazaal NM et al. The relationship between OXT gene polymorphisms and reproductive hormones in pregnant and lactating Awassi Ewes. Mol Biol Rep. 2023 Oct
Subcellular Location
Secreted.
UNIPROT
Synonyms
MGC126890 antibody
MGC126892 antibody
Neurophysin 1 antibody
Neurophysin I antibody
Ocytocin antibody
OT antibody
OT NPI antibody
OXT antibody
Oxytocin antibody
Oxytocin Neurophysin preproprotein antibody
ExpandImages
-
![Sandwich ELISA analysis of human Oxytocin-neurophysin 1 matched pair antibodies<br /><br />Capture: <a href="/products/HA723452" style="font-weight: bold;text-decoration: underline;">HA723452</a>, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-28]<br />Detector: <a href="/products/HA723453" style="font-weight: bold;text-decoration: underline;">HA723453</a>, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-29]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA723452" style="font-weight: bold;text-decoration: underline;">HA723452</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Oxytocin-neurophysin 1 protein (<a href="/products/HA211134" style="font-weight: bold;text-decoration: underline;">HA211134</a>) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723453" style="font-weight: bold;text-decoration: underline;">HA723453</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723454B_1.png?v=20260604174822)
Sandwich ELISA analysis of human Oxytocin-neurophysin 1 matched pair antibodies
Capture: HA723452, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-28]
Detector: HA723453, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-29]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723452) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Oxytocin-neurophysin 1 protein (HA211134) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA723453, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native Oxytocin-neurophysin 1 in human serum samples.<br /><br />Capture: <a href="/products/HA723452" style="font-weight: bold;text-decoration: underline;">HA723452</a>, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-28]<br />Detector: <a href="/products/HA723453" style="font-weight: bold;text-decoration: underline;">HA723453</a>, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-29]<br /><br />Interpolated concentration of native Oxytocin-neurophysin 1 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Oxytocin-neurophysin 1 concentration was determined to be 1368 pg/mL in Human serum.](https://storage.huabio.cn/huabio/productImg/HA723454B_2.png?v=20260604174822)
Interpolated concentrations of native Oxytocin-neurophysin 1 in human serum samples.
Capture: HA723452, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-28]
Detector: HA723453, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-29]
Interpolated concentration of native Oxytocin-neurophysin 1 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Oxytocin-neurophysin 1 concentration was determined to be 1368 pg/mL in Human serum. -
![Interpolated concentrations of spiked Oxytocin-neurophysin 1 in cell culture media samples.<br /><br />Capture: <a href="/products/HA723452" style="font-weight: bold;text-decoration: underline;">HA723452</a>, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-28]<br />Detector: <a href="/products/HA723453" style="font-weight: bold;text-decoration: underline;">HA723453</a>, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-29]<br /><br />The concentrations of Oxytocin-neurophysin 1 were measured in duplicates, interpolated from the Oxytocin-neurophysin 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).](https://storage.huabio.cn/huabio/productImg/HA723454B_3.png?v=20260604174822)
Interpolated concentrations of spiked Oxytocin-neurophysin 1 in cell culture media samples.
Capture: HA723452, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-28]
Detector: HA723453, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-29]
The concentrations of Oxytocin-neurophysin 1 were measured in duplicates, interpolated from the Oxytocin-neurophysin 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Human Oxytocin-neurophysin 1 Recombinant Rabbit Monoclonal Antibody [PSH12-29] - BSA and Azide free (Detector)
Application: ELISA(Det)
Reactivity: Human
Conjugate: unconjugated
