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KCNA5 / KV1-5 Recombinant Rabbit Monoclonal Antibody [PSH12-30]

Usd: 385

Specification

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Catalog# HA723455

KCNA5 / KV1-5 Recombinant Rabbit Monoclonal Antibody [PSH12-30]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

KCNA5 / KV1-5 Recombinant Rabbit Monoclonal Antibody [PSH12-30]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within rat KCNA5 aa 249-298 / 602.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 67 kDa

Positive Control

PANC-1 cell lysate, Mouse hippocampus tissue lysate, Mouse heart tissue lysate, Rat hippocampus tissue lysate, Rat heart tissue lysate.

Conjugation

unconjugated

Clone Number

PSH12-30

Reactivity Data

WB
Human
Tested Tested
Mouse
Tested Tested
Rat
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

Target

Function

KCNA5 is a voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes. It forms tetrameric, potassium-selective channels, allowing potassium ions to pass according to their electrochemical gradient. The channel alternates between open and closed states in response to changes in membrane voltage, contributing to the regulation of electrical activity in cells. KCNA5 can form functional homotetrameric channels or heterotetrameric channels with other KCNA family members, such as KCNA1, KCNA2, KCNA4, and KCNA5, which influence channel properties. This channel is critical for normal electrical conduction, including the formation of the ventricular conduction system and action potential configuration. It may also play a role in regulating insulin secretion in pancreatic islets. KCNA5 is associated with delayed rectifier potassium currents, which help restore the resting membrane potential after depolarization, particularly in beta cells. Defects in KCNA5 are linked to familial atrial fibrillation (ATFB7), a condition characterized by irregular heart rhythms that can lead to stroke or heart failure. Additionally, KCNA5 is implicated in pulmonary vascular function, where it helps set the resting membrane potential and participates in hypoxic pulmonary vasoconstriction.

Background References

1. Williams CP, et al. Modulation of the human Kv1.5 channel by protein kinase C activation: role of the Kvbeta1.2 subunit. J Pharmacol Exp Ther. 2002 Aug.

2. Olson TM, et al. Kv1.5 channelopathy due to KCNA5 loss-of-function mutation causes human atrial fibrillation. Hum Mol Genet. 2006 Jul.

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P22460 Human

SwissProt: Q61762 Mouse

SwissProt: P19024 Rat

Synonyms

ATFB7 antibody

cardiac potassium channel antibody

HCK1 antibody

HK2 antibody

HPCN1 antibody

insulinoma and islet potassium channel antibody

KCNA5 antibody

KCNA5_HUMAN antibody

KV1.5 antibody

PCN1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of KCNA5 / KV1-5 on different lysates with Rabbit anti-KCNA5 / KV1-5 antibody (<a href="/products/HA723455" style="font-weight: bold;text-decoration: underline;">HA723455</a>) at 1/1,000 dilution.<br /><br />Lane 1: PANC-1 cell lysate(no heat) (15 µg/Lane)<br />Lane 2: Mouse hippocampus tissue lysate (no heat) (20 µg/Lane)<br />Lane 3: Mouse heart tissue lysate (no heat) (20 µg/Lane)<br />Lane 4: Mouse liver tissue lysate (negative) (20 µg/Lane)<br />Lane 5: Rat hippocampus tissue lysate (20 µg/Lane)<br />Lane 6: Rat liver tissue lysate (negative) (20 µg/Lane)<br />Lane 7: Rat heart tissue lysate (20 µg/Lane)<br /><br />Notice: no heat means the lysate is not boiled.<br /><br />Predicted band size: 67 kDa<br />Observed band size: 67 kDa<br /><br />Exposure time: 1 minute 18 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723455" style="font-weight: bold;text-decoration: underline;">HA723455</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of KCNA5 / KV1-5 on different lysates with Rabbit anti-KCNA5 / KV1-5 antibody (HA723455) at 1/1,000 dilution.

    Lane 1: PANC-1 cell lysate(no heat) (15 µg/Lane)
    Lane 2: Mouse hippocampus tissue lysate (no heat) (20 µg/Lane)
    Lane 3: Mouse heart tissue lysate (no heat) (20 µg/Lane)
    Lane 4: Mouse liver tissue lysate (negative) (20 µg/Lane)
    Lane 5: Rat hippocampus tissue lysate (20 µg/Lane)
    Lane 6: Rat liver tissue lysate (negative) (20 µg/Lane)
    Lane 7: Rat heart tissue lysate (20 µg/Lane)

    Notice: no heat means the lysate is not boiled.

    Predicted band size: 67 kDa
    Observed band size: 67 kDa

    Exposure time: 1 minute 18 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723455) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"