Human TSLP Recombinant Rabbit Monoclonal Antibody [PSH12-88] - BSA and Azide free (Detector)
Overview
Product Name
Human TSLP Recombinant Rabbit Monoclonal Antibody [PSH12-88] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human TSLP aa 29-159 (HA210952).
Species Reactivity
Human
Validated Applications
ELISA(Det)
Positive Control
Recombinant Human TSLP protein (HA210952).
Conjugation
unconjugated
Clone Number
PSH12-88
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-87] to Human TSLP antibody (Capture) (HA723487) and recombinant Human TSLP protein (HA210952) as the standard. The reference range value is 7.8-1,000 pg/mL.
Target
Function
This gene encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2 (TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. The protein is therefore considered a potential therapeutic target for the treatment of such diseases. In addition, the shorter (predominant) isoform is an antimicrobial protein, displaying antibacterial and antifungal activity against B. cereus, E. coli, E. faecalis, S. mitis, S. epidermidis, and C. albicans. Alternative splicing of this gene results in multiple transcript variants.
Background References
1. O\'Byrne PM et al. Development of an inhaled anti-TSLP therapy for asthma. Pulm Pharmacol Ther. 2023 Feb
2. Nakajima S et al. Anti-TSLP antibodies: Targeting a master regulator of type 2 immune responses. Allergol Int. 2020 Apr
Subcellular Location
Secreted.
UNIPROT
Synonyms
Thymic stromal lymphopoietin antibody
Thymic stromal lymphopoietin protein TSLP antibody
Tslp antibody
TSLP protein antibody
TSLP_HUMAN antibody
Images
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![Sandwich ELISA analysis of Human TSLP matched pair antibodies<br /><br />Capture: <a href="/products/HA723487" style="font-weight: bold;text-decoration: underline;">HA723487</a>, Human TSLP Rabbit mAb [PSH12-87]<br />Detector: <a href="/products/HA723488" style="font-weight: bold;text-decoration: underline;">HA723488</a>, Human TSLP Rabbit mAb [PSH12-88]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (<a href="/products/HA723487" style="font-weight: bold;text-decoration: underline;">HA723487</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human TSLP protein (<a href="/products/HA210952" style="font-weight: bold;text-decoration: underline;">HA210952</a>) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723488" style="font-weight: bold;text-decoration: underline;">HA723488</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723488_1.png?v=20260429161945)
Sandwich ELISA analysis of Human TSLP matched pair antibodies
Capture: HA723487, Human TSLP Rabbit mAb [PSH12-87]
Detector: HA723488, Human TSLP Rabbit mAb [PSH12-88]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723487) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human TSLP protein (HA210952) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723488, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
Interpolated concentrations of spiked TSLP in human cell culture media samples.
The concentrations of TSLP were measured in duplicates, interpolated from the TSLPstandard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"