Biotin Conjugated Human VEGFA Recombinant Rabbit Monoclonal Antibody [PSH12-92] - Detector
Overview
Product Name
Biotin Conjugated Human VEGFA Recombinant Rabbit Monoclonal Antibody [PSH12-92] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human VEGF aa 27-147 (HA210929).
Species Reactivity
Human, Dog
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Human VEGFA protein (HA210929).
Conjugation
Biotin
Clone Number
PSH12-92
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
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Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-91] to Human VEGFA antibody (Capture) (HA723493) and recombinant Human VEGFA protein (HA210929) as the standard. The reference range value is 31.3-4,000 pg/mL.
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ELISA
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Use at an assay dependent concentration.
Target
Function
Vascular endothelial growth factor A (VEGF-A) is a protein that in humans is encoded by the VEGFA gene. This gene is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. VEGF-A shows prominent activity with vascular endothelial cells, primarily through its interactions with the VEGFR1 and -R2 receptors found prominently on the endothelial cell membrane. However, it does have effects on a number of other cell types (e.g., stimulation monocyte/macrophage migration, neurons, cancer cells, kidney and epithelial cells ). In vitro, VEGF-A has been shown to stimulate endothelial cell mitogenesis and cell migration. VEGF-A is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor.
Background References
1. Rizvi F et al. VEGFA mRNA-LNP promotes biliary epithelial cell-to-hepatocyte conversion in acute and chronic liver diseases and reverses steatosis and fibrosis. Cell Stem Cell. 2023 Dec
2. White AL et al. VEGFA Isoforms as Pro-Angiogenic Therapeutics for Cerebrovascular Diseases. Biomolecules. 2023 Apr
Subcellular Location
Secreted.
UNIPROT
Synonyms
Folliculostellate cell-derived growth factor antibody
Glioma-derived endothelial cell mitogen antibody
MGC70609 antibody
MVCD1 antibody
Vascular endothelial growth factor A antibody
vascular endothelial growth factor A121 antibody
vascular endothelial growth factor A165 antibody
vascular endothelial growth factor antibody
Vascular permeability factor antibody
VEGF A antibody
ExpandImages
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![Sandwich ELISA analysis of Human VEGFA matched pair antibodies<br /><br />Capture: <a href="/products/HA723493" style="font-weight: bold;text-decoration: underline;">HA723493</a>, Human VEGFA Rabbit mAb [PSH12-91]<br />Detector: <a href="/products/HA723494" style="font-weight: bold;text-decoration: underline;">HA723494</a>, Human VEGFA Rabbit mAb [PSH12-92]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (<a href="/products/HA723493" style="font-weight: bold;text-decoration: underline;">HA723493</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human VEGFA protein (<a href="/products/HA210929" style="font-weight: bold;text-decoration: underline;">HA210929</a>) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723494" style="font-weight: bold;text-decoration: underline;">HA723494</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723495B_1.png?v=20260604174818)
Sandwich ELISA analysis of Human VEGFA matched pair antibodies
Capture: HA723493, Human VEGFA Rabbit mAb [PSH12-91]
Detector: HA723494, Human VEGFA Rabbit mAb [PSH12-92]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723493) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human VEGFA protein (HA210929) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723494, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native VEGFA in A431 and HepG2 cell culture supernatant.<br /><br />Capture: <a href="/products/HA723493" style="font-weight: bold;text-decoration: underline;">HA723493</a>, Human VEGFA Rabbit mAb [PSH12-91]<br />Detector: <a href="/products/HA723494" style="font-weight: bold;text-decoration: underline;">HA723494</a>, Human VEGFA Rabbit mAb [PSH12-92]<br /><br />The concentrations of VEGFA measured in duplicate and interpolated from the VEGFA standard curve and corrected for sample dilution. Undiluted samples are as follows: A431 cell culture supernatant 12.5% and HepG2 cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGFA concentration was determined to be 6,033 pg/ml in A431 cell culture supernatant and 3,949 pg/ml in HepG2 cell culture supernatant.](https://storage.huabio.cn/huabio/productImg/HA723495B_2.png?v=20260604174818)
Interpolated concentrations of native VEGFA in A431 and HepG2 cell culture supernatant.
Capture: HA723493, Human VEGFA Rabbit mAb [PSH12-91]
Detector: HA723494, Human VEGFA Rabbit mAb [PSH12-92]
The concentrations of VEGFA measured in duplicate and interpolated from the VEGFA standard curve and corrected for sample dilution. Undiluted samples are as follows: A431 cell culture supernatant 12.5% and HepG2 cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGFA concentration was determined to be 6,033 pg/ml in A431 cell culture supernatant and 3,949 pg/ml in HepG2 cell culture supernatant. -
![Interpolated concentrations of spiked VEGFA in human cell culture media samples.<br /><br />Capture: <a href="/products/HA723493" style="font-weight: bold;text-decoration: underline;">HA723493</a>, Human VEGFA Rabbit mAb [PSH12-91]<br />Detector: <a href="/products/HA723494" style="font-weight: bold;text-decoration: underline;">HA723494</a>, Human VEGFA Rabbit mAb [PSH12-92]<br /><br />The concentrations of VEGFA were measured in duplicates, interpolated from the VEGFAstandard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).](https://storage.huabio.cn/huabio/productImg/HA723495B_3.png?v=20260604174818)
Interpolated concentrations of spiked VEGFA in human cell culture media samples.
Capture: HA723493, Human VEGFA Rabbit mAb [PSH12-91]
Detector: HA723494, Human VEGFA Rabbit mAb [PSH12-92]
The concentrations of VEGFA were measured in duplicates, interpolated from the VEGFAstandard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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