Decorin Recombinant Rabbit Monoclonal Antibody [PSH12-99]
Catalog# HA723504
Decorin Recombinant Rabbit Monoclonal Antibody [PSH12-99]
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WB
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IHC-P
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Human
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Mouse
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Rat
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HA751454
不含抗保成分
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unconjugated
Overview
Product Name
Decorin Recombinant Rabbit Monoclonal Antibody [PSH12-99]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Decorin aa 1-359.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 40 kDa
Positive Control
Mouse colon tissue lysate, Rat kidney tissue lysate, Rat colon tissue lysate, Rat placenta tissue lysate, human heart tissue, human kidney tissue, mouse heart tissue, mouse kidney tissue, rat heart tissue, rat kidney tissue.
Conjugation
unconjugated
Clone Number
PSH12-99
Reactivity Data
| WB | IHC-P | |
|---|---|---|
| Human |
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| Mouse |
|
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| Rat |
|
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IHC-P
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1:100-1:200
Target
Function
Decorin is a protein that in humans is encoded by the DCN gene. Decorin is a proteoglycan that is on average 90 - 140 kilodaltons (kDa) in molecular weight. It belongs to the small leucine-rich proteoglycan (SLRP) family and consists of a protein core containing leucine repeats with a glycosaminoglycan (GAG) chain consisting of either chondroitin sulfate (CS) or dermatan sulfate (DS). Decorin is a small cellular or pericellular matrix proteoglycan and is closely related in structure to biglycan protein. Decorin and biglycan are thought to be the result of a gene duplication. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly.
Background References
1. Wang L et al. Decorin promotes decidual M1-like macrophage polarization via mitochondrial dysfunction resulting in recurrent pregnancy loss. Theranostics. 2022 Oct
2. Lin Y et al. Bifidobacterium adolescentis induces Decorin(+) macrophages via TLR2 to suppress colorectal carcinogenesis. J Exp Clin Cancer Res. 2023 Jul
Subcellular Location
Secreted, extracellular space, extracellular matrix.
Synonyms
Bone proteoglycan II antibody
CSCD antibody
DCN antibody
DCN protein antibody
Decorin antibody
Decorin proteoglycan antibody
Dermatan sulphate proteoglycans II antibody
DKFZp686J19238 antibody
DSPG 2 antibody
DSPG2 antibody
ExpandImages
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Western blot analysis of Decorin on different lysates with Rabbit anti-Decorin antibody (HA723504) at 1/5,000 dilution.
Lane 1: Mouse colon tissue lysate (40 µg/Lane)
Lane 2: Rat kidney tissue lysate (40 µg/Lane)
Lane 3: Rat colon tissue lysate (40 µg/Lane)
Lane 4: Rat placenta tissue lysate (40 µg/Lane)
Predicted band size: 40 kDa
Observed band size: 40-75 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723504) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-Decorin antibody (HA723504) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723504) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Decorin antibody (HA723504) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723504) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Decorin antibody (HA723504) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723504) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Decorin antibody (HA723504) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723504) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-Decorin antibody (HA723504) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723504) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Decorin antibody (HA723504) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723504) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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