Human CCL17 / TARC Recombinant Rabbit Monoclonal Antibody [PSH13-35] - BSA and Azide free (Detector)
Overview
Product Name
Human CCL17 / TARC Recombinant Rabbit Monoclonal Antibody [PSH13-35] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human CCL17 aa 24-94 (HA210895).
Species Reactivity
Human
Validated Applications
ELISA(Det)
Positive Control
Recombinant Human CCL17 / TARC protein (HA210895).
Conjugation
unconjugated
Clone Number
PSH13-35
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH13-33] to Human CCL17 / TARC antibody (Capture) (HA723513) and recombinant Human CCL17 / TARC protein (HA210895) as the standard. The reference range value is 31.3-2,000 pg/mL.
Target
Function
Chemokine, which displays chemotactic activity for T lymphocytes, preferentially Th2 cells, but not monocytes or granulocytes. Therefore plays an important role in a wide range of inflammatory and immunological processes. In the brain, required to maintain the typical, highly branched morphology of hippocampal microglia under homeostatic conditions. May be important for the appropriate adaptation of microglial morphology and synaptic plasticity to acute lipopolysaccharide (LPS)-induced neuroinflammation. Plays a role in wound healing, mainly by inducing fibroblast migration into the wound.
Background References
1. Feng G et al. CCL17 Aggravates Myocardial Injury by Suppressing Recruitment of Regulatory T Cells. Circulation. 2022 Mar
2. Lupancu TJ et al. CCL17/TARC in autoimmunity and inflammation-not just a T-cell chemokine. Immunol Cell Biol. 2023 Aug
Subcellular Location
Secreted.
UNIPROT
Synonyms
A-152E5.3 antibody
A152E53 antibody
ABCD 2 antibody
ABCD2 antibody
C-C motif chemokine 17 antibody
CC chemokine TARC antibody
CCL17 antibody
CCL17_HUMAN antibody
Chemokine CC Motif Ligand 17 antibody
MGC138271 antibody
ExpandImages
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![Sandwich ELISA analysis of Human CCL17 / TARC matched pair antibodies<br /><br />Capture: <a href="/products/HA723513" style="font-weight: bold;text-decoration: underline;">HA723513</a>, Human CCL17 / TARC Rabbit mAb [PSH13-33]<br />Detector: <a href="/products/HA723516" style="font-weight: bold;text-decoration: underline;">HA723516</a>, Human CCL17 / TARC Rabbit mAb [PSH13-35]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA723513" style="font-weight: bold;text-decoration: underline;">HA723513</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL17 / TARC protein (<a href="/products/HA210895" style="font-weight: bold;text-decoration: underline;">HA210895</a>) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723516" style="font-weight: bold;text-decoration: underline;">HA723516</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723516_1.png?v=20260401002324)
Sandwich ELISA analysis of Human CCL17 / TARC matched pair antibodies
Capture: HA723513, Human CCL17 / TARC Rabbit mAb [PSH13-33]
Detector: HA723516, Human CCL17 / TARC Rabbit mAb [PSH13-35]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723513) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL17 / TARC protein (HA210895) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723516, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native CCL17 in human PBMC cell culture supernatant.<br /><br />Capture: <a href="/products/HA723513" style="font-weight: bold;text-decoration: underline;">HA723513</a>, Human CCL17 / TARC Rabbit mAb [PSH13-33]<br />Detector: <a href="/products/HA723516" style="font-weight: bold;text-decoration: underline;">HA723516</a>, Human CCL17 / TARC Rabbit mAb [PSH13-35]<br /><br />PBMC cells were stimulated with 10 µg/ml PHA-M and incubated for 5 days. The concentrations of CCL17 measured in duplicate and interpolated from the CCL17 standard curve and corrected for sample dilution. Undiluted samples are as follows: stimulated 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL17 concentration was determined to be 8,300 pg/ml in PHA-M stimulated PBMC cell culture supernatant.](https://storage.huabio.cn/huabio/productImg/HA723516_2.png?v=20260401002324)
Interpolated concentrations of native CCL17 in human PBMC cell culture supernatant.
Capture: HA723513, Human CCL17 / TARC Rabbit mAb [PSH13-33]
Detector: HA723516, Human CCL17 / TARC Rabbit mAb [PSH13-35]
PBMC cells were stimulated with 10 µg/ml PHA-M and incubated for 5 days. The concentrations of CCL17 measured in duplicate and interpolated from the CCL17 standard curve and corrected for sample dilution. Undiluted samples are as follows: stimulated 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL17 concentration was determined to be 8,300 pg/ml in PHA-M stimulated PBMC cell culture supernatant. -
![Interpolated concentrations of spiked CCL17 in human cell culture media samples.<br /><br />Capture: <a href="/products/HA723513" style="font-weight: bold;text-decoration: underline;">HA723513</a>, Human CCL17 / TARC Rabbit mAb [PSH13-33]<br />Detector: <a href="/products/HA723516" style="font-weight: bold;text-decoration: underline;">HA723516</a>, Human CCL17 / TARC Rabbit mAb [PSH13-35]<br /><br />The concentrations of CCL17 were measured in duplicates, interpolated from the CCL17 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).](https://storage.huabio.cn/huabio/productImg/HA723516_3.png?v=20260401002324)
Interpolated concentrations of spiked CCL17 in human cell culture media samples.
Capture: HA723513, Human CCL17 / TARC Rabbit mAb [PSH13-33]
Detector: HA723516, Human CCL17 / TARC Rabbit mAb [PSH13-35]
The concentrations of CCL17 were measured in duplicates, interpolated from the CCL17 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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