Mouse IL-1 alpha Recombinant Rabbit Monoclonal Antibody [PSH13-43] - BSA and Azide free (Detector)
Overview
Product Name
Mouse IL-1 alpha Recombinant Rabbit Monoclonal Antibody [PSH13-43] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mouse IL1 alpha aa 115-270 (HA210971).
Species Reactivity
Mouse
Validated Applications
ELISA(Det)
Positive Control
Recombinant Mouse IL-1 alpha protein (HA210971).
Conjugation
unconjugated
Clone Number
PSH13-43
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH13-42] to Mouse IL-1 alpha antibody (Capture) (HA723529) and recombinant Mouse IL-1 alpha protein (HA210971) as the standard. The reference range value is 4.9-1,200 pg/mL.
Target
Function
Interleukin-1 alpha (IL-1 alpha) also known as hematopoietin 1 is a cytokine of the interleukin 1 family that in humans is encoded by the IL1A gene. In general, Interleukin 1 is responsible for the production of inflammation, as well as the promotion of fever and sepsis. IL-1α inhibitors are being developed to interrupt those processes and treat diseases. IL-1α is produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. It is on the pathway that activates tumor necrosis factor-alpha.
Background References
1. Harati-Sadegh M et al. IL1A and IL1B gene polymorphisms and keratoconus susceptibility: evidence from an updated meta-analysis. Ophthalmic Genet. 2021 Oct
2. Zhang N et al. IRF1 and IL1A associated with PANoptosis serve as potential immune signatures for lung ischemia reperfusion injury following lung transplantation. Int Immunopharmacol. 2024 Sep
Subcellular Location
Secreted.
UNIPROT
Synonyms
BAF antibody
FAF antibody
Hematopoietin 1 antibody
Hematopoietin-1 antibody
IL 1 alpha antibody
IL 1A antibody
IL-1 alpha antibody
Il-1a antibody
IL1 ALPHA antibody
IL1 antibody
ExpandImages
-
![Sandwich ELISA analysis of Mouse IL-1 alpha matched pair antibodies<br /><br />Capture: <a href="/products/HA723526" style="font-weight: bold;text-decoration: underline;">HA723526</a>, Mouse IL-1 alpha Rabbit mAb [PSH13-42]<br />Detector: <a href="/products/HA723527" style="font-weight: bold;text-decoration: underline;">HA723527</a>, Mouse IL-1 alpha Rabbit mAb [PSH13-43]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA723529" style="font-weight: bold;text-decoration: underline;">HA723529</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-1 alpha protein (<a href="/products/HA210971" style="font-weight: bold;text-decoration: underline;">HA210971</a>) starting from 1,200 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723530" style="font-weight: bold;text-decoration: underline;">HA723530</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723530_1.png?v=20260401002330)
Sandwich ELISA analysis of Mouse IL-1 alpha matched pair antibodies
Capture: HA723526, Mouse IL-1 alpha Rabbit mAb [PSH13-42]
Detector: HA723527, Mouse IL-1 alpha Rabbit mAb [PSH13-43]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723529) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-1 alpha protein (HA210971) starting from 1,200 pg/ml to 0 pg/ml and detect antibody (HA723530, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native IL-1 alpha in Raw264.7 supernatant treated or untreated with LPS for 48 hours.<br /><br />Capture: <a href="/products/HA723529" style="font-weight: bold;text-decoration: underline;">HA723529</a>, Mouse IL-1 alpha Rabbit mAb [PSH13-42]<br />Detector: <a href="/products/HA723530" style="font-weight: bold;text-decoration: underline;">HA723530</a>, Mouse IL-1 alpha Rabbit mAb [PSH13-43]<br /><br />Interpolated concentration of native IL-1 alpha was measured in duplicate at different sample concentrations. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean IL-1 alpha concentration was determined to be 1,185 pg/mL in neat Raw264.7 treated supernatant, undetectable in untreated Raw264.7 supernatant.](https://storage.huabio.cn/huabio/productImg/HA723530_2.png?v=20260401002330)
Interpolated concentrations of native IL-1 alpha in Raw264.7 supernatant treated or untreated with LPS for 48 hours.
Capture: HA723529, Mouse IL-1 alpha Rabbit mAb [PSH13-42]
Detector: HA723530, Mouse IL-1 alpha Rabbit mAb [PSH13-43]
Interpolated concentration of native IL-1 alpha was measured in duplicate at different sample concentrations. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean IL-1 alpha concentration was determined to be 1,185 pg/mL in neat Raw264.7 treated supernatant, undetectable in untreated Raw264.7 supernatant. -
![Interpolated concentrations of spiked IL-1 alpha in cell culture media samples.<br /><br />Capture: <a href="/products/HA723529" style="font-weight: bold;text-decoration: underline;">HA723529</a>, Mouse IL-1 alpha Rabbit mAb [PSH13-42]<br />Detector: <a href="/products/HA723530" style="font-weight: bold;text-decoration: underline;">HA723530</a>, Mouse IL-1 alpha Rabbit mAb [PSH13-43]<br /><br />The concentrations of IL-1 alpha were measured in duplicates, interpolated from the IL-1 alpha standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).](https://storage.huabio.cn/huabio/productImg/HA723530_3.png?v=20260401002330)
Interpolated concentrations of spiked IL-1 alpha in cell culture media samples.
Capture: HA723529, Mouse IL-1 alpha Rabbit mAb [PSH13-42]
Detector: HA723530, Mouse IL-1 alpha Rabbit mAb [PSH13-43]
The concentrations of IL-1 alpha were measured in duplicates, interpolated from the IL-1 alpha standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"