Dlx2 Recombinant Rabbit Monoclonal Antibody [PSH13-46]
Catalog# HA723535
Dlx2 Recombinant Rabbit Monoclonal Antibody [PSH13-46]
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WB
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Mouse
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Rat
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unconjugated
Overview
Product Name
Dlx2 Recombinant Rabbit Monoclonal Antibody [PSH13-46]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Dlx2 aa 1-328.
Species Reactivity
Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 34 kDa
Positive Control
Neuro-2a cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
PSH13-46
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | IHC-Fr | FC | IF-Cell | |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
Target
Function
Homeobox protein DLX-2 is a protein that in humans is encoded by the DLX2 gene. Many vertebrate homeo box-containing genes have been identified on the basis of their sequence similarity with Drosophila developmental genes. Members of the Dlx gene family contain a homeobox that is related to that of Distal-less (Dll), a gene expressed in the head and limbs of the developing fruit fly. The Distal-less (Dlx) family of genes comprises at least 6 different members, DLX1-DLX6. The DLX proteins are postulated to play a role in forebrain and craniofacial development. This gene is located in a tail-to-tail configuration with another member of the gene family on the long arm of chromosome 2.
Background References
1. Zhang B et al. DLX2 promotes osteosarcoma epithelial-mesenchymal transition and doxorubicin resistance by enhancing HOXC8-CDH2 axis. iScience. 2023 Oct
2. Zeng X et al. DLX2 activates Wnt1 transcription and mediates Wnt/beta-catenin signal to promote osteogenic differentiation of hBMSCs. Gene. 2020 Jun
Subcellular Location
Nucleus.
Synonyms
DII A antibody
Distal less homeo box 2 antibody
Distal less homeobox 2 antibody
DLX 2 antibody
Dlx2 antibody
DLX2_HUMAN antibody
Dlx5 antibody
Homeobox protein DLX 2 antibody
Homeobox protein DLX-2 antibody
Homeobox protein DLX2 antibody
ExpandImages
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Western blot analysis of Dlx2 on different lysates with Rabbit anti-Dlx2 antibody (HA723535) at 1/2,000 dilution.
Lane 1: Neuro-2a cell lysate (20 µg/Lane)
Lane 2: Mouse brain tissue lysate (40 µg/Lane)
Lane 3: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 34 kDa
Observed band size: 45 kDa
Exposure time: 2 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723535) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"