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CaV2.3 / CACNA1E Recombinant Rabbit Monoclonal Antibody [PSH13-51]

Usd: 385

Specification

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Catalog# HA723540

CaV2.3 / CACNA1E Recombinant Rabbit Monoclonal Antibody [PSH13-51]

Application

  • IHC-Fr

  • IHC-P

  • WB

Reactivity

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

CaV2.3 / CACNA1E Recombinant Rabbit Monoclonal Antibody [PSH13-51]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human CACNA1E aa 1,964-2,313.

Species Reactivity

Mouse, Rat

Validated Applications

IHC-Fr, IHC-P, WB

Molecular Weight

Predicted band size: 262 kDa

Positive Control

Mouse striatum tissue, mouse hippocampus tissue, rat hippocampus tissue, Mouse brain tissue lysate, Mouse hippocampus tissue lysate, Rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

PSH13-51

Reactivity Data

IHC-Fr IHC-P WB
Mouse
Tested Tested
Tested Tested
Tested Tested
Rat
Not Recommended
Tested Tested
Tested Tested
Human
Not Recommended
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-Fr

  • 1:500

  • IHC-P

  • 1:3,000-1:6,000

  • WB

  • 1:5,000

Target

Function

The R-type calcium channel is a type of voltage-dependent calcium channel. Like the others of this class, the α1 subunit forms the pore through which calcium enters the cell and determines most of the channel's properties. This α1 subunit is also known as the calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E) or Cav2.3 which in humans is encoded by the CACNA1E gene. They are strongly expressed in cortex, hippocampus, striatum, amygdala and interpeduncular nucleus. They are poorly understood, but like Q-type calcium channels, they appear to be present in cerebellar granule cells. They have a high threshold of activation and relatively slow kinetics.

Background References

1. Schneider T et al. Cav2.3 R-type calcium channels: from its discovery to pathogenic de novo CACNA1E variants: a historical perspective. Pflugers Arch. 2020 Jul

2. Royer-Bertrand B et al. De novo variants in CACNA1E found in patients with intellectual disability, developmental regression and social cognition deficit but no seizures. Mol Autism. 2021 Oct

Subcellular Location

Membrane.

UNIPROT

SwissProt: Q61290 Mouse

SwissProt: Q07652 Rat

Synonyms

alpha-1 polypeptide antibody

BII antibody

Brain calcium channel II antibody

CAC 1E antibody

CAC1E antibody

CAC1E_HUMAN antibody

CACH 6 antibody

CACH6 antibody

CACNA1E antibody

Cacnl1 a6 antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: hippocampus<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: hippocampus

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Rabbit anti-CaV2.3 / CACNA1E antibody (<a href="/products/HA723540" style="font-weight: bold;text-decoration: underline;">HA723540</a>) at 1/3,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723540" style="font-weight: bold;text-decoration: underline;">HA723540</a>) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Rabbit anti-CaV2.3 / CACNA1E antibody (HA723540) at 1/3,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723540) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-CaV2.3 / CACNA1E antibody (<a href="/products/HA723540" style="font-weight: bold;text-decoration: underline;">HA723540</a>) at 1/6,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723540" style="font-weight: bold;text-decoration: underline;">HA723540</a>) at 1/6,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-CaV2.3 / CACNA1E antibody (HA723540) at 1/6,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723540) at 1/6,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded mouse kidney tissue (negative) with Rabbit anti-CaV2.3 / CACNA1E antibody (<a href="/products/HA723540" style="font-weight: bold;text-decoration: underline;">HA723540</a>) at 1/3,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723540" style="font-weight: bold;text-decoration: underline;">HA723540</a>) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue (negative) with Rabbit anti-CaV2.3 / CACNA1E antibody (HA723540) at 1/3,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723540) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-CaV2.3 / CACNA1E antibody (<a href="/products/HA723540" style="font-weight: bold;text-decoration: underline;">HA723540</a>) at 1/6,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723540" style="font-weight: bold;text-decoration: underline;">HA723540</a>) at 1/6,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-CaV2.3 / CACNA1E antibody (HA723540) at 1/6,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723540) at 1/6,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded rat kidney tissue (negative) with Rabbit anti-CaV2.3 / CACNA1E antibody (<a href="/products/HA723540" style="font-weight: bold;text-decoration: underline;">HA723540</a>) at 1/3,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723540" style="font-weight: bold;text-decoration: underline;">HA723540</a>) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue (negative) with Rabbit anti-CaV2.3 / CACNA1E antibody (HA723540) at 1/3,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723540) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of CaV2.3 / CACNA1E on different lysates with Rabbit anti-CaV2.3 / CACNA1E antibody (<a href="/products/HA723540" style="font-weight: bold;text-decoration: underline;">HA723540</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate (no heat)<br />Lane 2: Mouse hippocampus tissue lysate (no heat)<br />Lane 3: Mouse lung tissue lysate (negative) (no heat)<br />Lane 4: Rat brain tissue lysate (no heat)<br /><br />Notice: no heat means the lysate is not boiled.<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 262 kDa<br />Observed band size: 257 kDa<br /><br />Exposure time: 46 seconds; ECL: K1801;<br /><br />3-8% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723540" style="font-weight: bold;text-decoration: underline;">HA723540</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of CaV2.3 / CACNA1E on different lysates with Rabbit anti-CaV2.3 / CACNA1E antibody (HA723540) at 1/5,000 dilution.

    Lane 1: Mouse brain tissue lysate (no heat)
    Lane 2: Mouse hippocampus tissue lysate (no heat)
    Lane 3: Mouse lung tissue lysate (negative) (no heat)
    Lane 4: Rat brain tissue lysate (no heat)

    Notice: no heat means the lysate is not boiled.

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 262 kDa
    Observed band size: 257 kDa

    Exposure time: 46 seconds; ECL: K1801;

    3-8% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723540) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

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