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AMACR Recombinant Rabbit Monoclonal Antibody [PSH13-54]

Usd: 385

Specification

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Catalog# HA723543

AMACR Recombinant Rabbit Monoclonal Antibody [PSH13-54]

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

AMACR Recombinant Rabbit Monoclonal Antibody [PSH13-54]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human AMACR aa 1-382.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 42 kDa

Positive Control

LNCaP cell lysate, U-2 OS cell lysate, A375 cell lysate, Mouse kidney tissue lysate, human prostate cancer tissue, human renal cell carcinoma tissue, human kidney tissue.

Conjugation

unconjugated

Clone Number

PSH13-54

Reactivity Data

WB IHC-P IF-Tissue
Human
Tested Tested
Tested Tested
Mouse
Tested Tested
Not Recommended
Rat
Not Recommended
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:10,000

  • IF-Tissue

  • 1:1,000

Target

Function

α-Methylacyl-CoA racemase (AMACR, EC 5.1.99.4) is an enzyme that in humans is encoded by the AMACR gene. In mammalian cells, the enzyme is responsible for converting (2R)-methylacyl-CoA esters to their (2S)-methylacyl-CoA epimers and known substrates, including coenzyme A esters of pristanic acid (mostly derived from phytanic acid, a 3-methyl branched-chain fatty acid that is abundant in the diet) and bile acids derived from cholesterol. This transformation is required in order to degrade (2R)-methylacyl-CoA esters by β-oxidation, which process requires the (2S)-epimer. The enzyme is known to be localised in peroxisomes and mitochondria, both of which are known to β-oxidize 2-methylacyl-CoA esters.

Background References

1. Lerner G et al. AMACR Expression is a Potential Diagnostic Marker in Apocrine Lesions of Breast, and is Associated with High Histologic Grade and Lymph Node Metastases in Some Invasive Apocrine Breast Cancers. Clin Breast Cancer. 2023 Feb

2. Travaglino A et al. Diagnostic accuracy of HNF1beta, Napsin A and P504S/Alpha-Methylacyl-CoA Racemase (AMACR) as markers of endometrial clear cell carcinoma. Pathol Res Pract. 2022 Sep

Subcellular Location

Peroxisome, Mitochondrion.

UNIPROT

SwissProt: Q9UHK6 Human

SwissProt: O09174 Mouse

Synonyms

2 arylpropionyl CoA epimerase antibody

2 methylacyl CoA racemase antibody

2-methylacyl-CoA racemase antibody

Alpha methylacyl CoA racemase antibody

Alpha methylacyl Coenzyme A racemase antibody

Alpha methylacyl-CoA racemase deficiency, included antibody

Alpha-methylacyl-CoA racemase antibody

Amacr antibody

AMACR deficiency, included antibody

AMACR_HUMAN antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of AMACR on different lysates with Rabbit anti-AMACR antibody (<a href="/products/HA723543" style="font-weight: bold;text-decoration: underline;">HA723543</a>) at 1/5,000 dilution.<br /><br />Lane 1: LNCaP cell lysate (20 µg/Lane)<br />Lane 2: U-2 OS cell lysate (low expression) (20 µg/Lane)<br />Lane 3: A375 cell lysate (20 µg/Lane)<br />Lane 4: Mouse kidney tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 42 kDa<br />Observed band size: 42 kDa<br /><br />Exposure time: 2 minute 22 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723543" style="font-weight: bold;text-decoration: underline;">HA723543</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of AMACR on different lysates with Rabbit anti-AMACR antibody (HA723543) at 1/5,000 dilution.

    Lane 1: LNCaP cell lysate (20 µg/Lane)
    Lane 2: U-2 OS cell lysate (low expression) (20 µg/Lane)
    Lane 3: A375 cell lysate (20 µg/Lane)
    Lane 4: Mouse kidney tissue lysate (40 µg/Lane)

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

    Exposure time: 2 minute 22 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723543) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-AMACR antibody (<a href="/products/HA723543" style="font-weight: bold;text-decoration: underline;">HA723543</a>) at 1/10,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723543" style="font-weight: bold;text-decoration: underline;">HA723543</a>) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-AMACR antibody (HA723543) at 1/10,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723543) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-AMACR antibody (<a href="/products/HA723543" style="font-weight: bold;text-decoration: underline;">HA723543</a>) at 1/10,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723543" style="font-weight: bold;text-decoration: underline;">HA723543</a>) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-AMACR antibody (HA723543) at 1/10,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723543) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue with Rabbit anti-AMACR antibody (<a href="/products/HA723543" style="font-weight: bold;text-decoration: underline;">HA723543</a>) at 1/10,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723543" style="font-weight: bold;text-decoration: underline;">HA723543</a>) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue with Rabbit anti-AMACR antibody (HA723543) at 1/10,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723543) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-AMACR antibody (<a href="/products/HA723543" style="font-weight: bold;text-decoration: underline;">HA723543</a>) at 1/10,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723543" style="font-weight: bold;text-decoration: underline;">HA723543</a>) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-AMACR antibody (HA723543) at 1/10,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723543) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IF-Tissue<br /><br />Species: Human<br /><br />Site: kidney<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/1,000

    Application: IF-Tissue

    Species: Human

    Site: kidney

    Sample: Paraffin-embedded section

    Antibody concentration: 1/1,000

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