Mouse CD86 Recombinant Rabbit Monoclonal Antibody [PSH13-64] - BSA and Azide free (Detector)
Overview
Product Name
Mouse CD86 Recombinant Rabbit Monoclonal Antibody [PSH13-64] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mouse CD68 aa 24-244 (HA210879).
Species Reactivity
Mouse
Validated Applications
ELISA(Det)
Positive Control
Recombinant Mouse CD86 protein (HA210879).
Conjugation
unconjugated
Clone Number
PSH13-64
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH13-63] to Mouse CD86 antibody (Capture) (HA723552) and recombinant Mouse CD86 protein (HA210879) as the standard. The reference range value is 7.8-1,000 pg/mL.
Target
Function
Cluster of Differentiation 86 (also known as CD86 and B7-2) is a protein constitutively expressed on dendritic cells, Langerhans cells, macrophages, B-cells (including memory B-cells), and on other antigen-presenting cells. Along with CD80, CD86 provides costimulatory signals necessary for T cell activation and survival. Depending on the ligand bound, CD86 can signal for self-regulation and cell-cell association, or for attenuation of regulation and cell-cell disassociation. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Alternative splicing results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined.
Background References
1. Tekguc M et al. Treg-expressed CTLA-4 depletes CD80/CD86 by trogocytosis, releasing free PD-L1 on antigen-presenting cells. Proc Natl Acad Sci U S A. 2021 Jul
2. Kennedy A et al. Differences in CD80 and CD86 transendocytosis reveal CD86 as a key target for CTLA-4 immune regulation. Nat Immunol. 2022 Sep
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
Activation B7-2 antigen 3 antibody
Activation B7-2 antigen antibody
B-lymphocyte activation antigen B7-2 2 antibody
B-lymphocyte activation antigen B7-2 antibody
B7 2 antibody
B7 antibody
B7-2 antibody
B7.2 antibody
B70 antibody
B72 antibody
ExpandImages
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![Sandwich ELISA analysis of Mouse CD86 matched pair antibodies<br /><br />Capture: <a href="/products/HA723552" style="font-weight: bold;text-decoration: underline;">HA723552</a>, Mouse CD86 Rabbit mAb [PSH13-63]<br />Detector: <a href="/products/HA723553" style="font-weight: bold;text-decoration: underline;">HA723553</a>, Mouse CD86 Rabbit mAb [PSH13-64]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA723552" style="font-weight: bold;text-decoration: underline;">HA723552</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CD86 protein (<a href="/products/HA210879" style="font-weight: bold;text-decoration: underline;">HA210879</a>) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723553" style="font-weight: bold;text-decoration: underline;">HA723553</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723553_1.png?v=20260401002340)
Sandwich ELISA analysis of Mouse CD86 matched pair antibodies
Capture: HA723552, Mouse CD86 Rabbit mAb [PSH13-63]
Detector: HA723553, Mouse CD86 Rabbit mAb [PSH13-64]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723552) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CD86 protein (HA210879) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723553, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native CD86 in mouse serum samples.<br /><br />Capture: <a href="/products/HA723552" style="font-weight: bold;text-decoration: underline;">HA723552</a>, Mouse CD86 Rabbit mAb [PSH13-63]<br />Detector: <a href="/products/HA723553" style="font-weight: bold;text-decoration: underline;">HA723553</a>, Mouse CD86 Rabbit mAb [PSH13-64]<br /><br />Interpolated concentration of native CD86 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD86 concentration was determined to be 999 pg/mL in Mouse serum.](https://storage.huabio.cn/huabio/productImg/HA723553_2.png?v=20260401002340)
Interpolated concentrations of native CD86 in mouse serum samples.
Capture: HA723552, Mouse CD86 Rabbit mAb [PSH13-63]
Detector: HA723553, Mouse CD86 Rabbit mAb [PSH13-64]
Interpolated concentration of native CD86 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD86 concentration was determined to be 999 pg/mL in Mouse serum. -
![Interpolated concentrations of native CD68 in J774A.1 Cell extract samples based on a 1000 µg/ml extract load.<br /><br />Capture: <a href="/products/HA723552" style="font-weight: bold;text-decoration: underline;">HA723552</a>, Mouse CD86 Rabbit mAb [PSH13-63]<br />Detector: <a href="/products/HA723553" style="font-weight: bold;text-decoration: underline;">HA723553</a>, Mouse CD86 Rabbit mAb [PSH13-64]<br /><br />Interpolated concentration of native CD86 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD86 concentration was determined to be 220 pg/mL in J774A.1 Cell extract.](https://storage.huabio.cn/huabio/productImg/HA723553_3.png?v=20260401002340)
Interpolated concentrations of native CD68 in J774A.1 Cell extract samples based on a 1000 µg/ml extract load.
Capture: HA723552, Mouse CD86 Rabbit mAb [PSH13-63]
Detector: HA723553, Mouse CD86 Rabbit mAb [PSH13-64]
Interpolated concentration of native CD86 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD86 concentration was determined to be 220 pg/mL in J774A.1 Cell extract. -
![Interpolated concentrations of spiked CD86 in cell culture media samples.<br /><br />Capture: <a href="/products/HA723552" style="font-weight: bold;text-decoration: underline;">HA723552</a>, Mouse CD86 Rabbit mAb [PSH13-63]<br />Detector: <a href="/products/HA723553" style="font-weight: bold;text-decoration: underline;">HA723553</a>, Mouse CD86 Rabbit mAb [PSH13-64]<br /><br />The concentrations of CD86 were measured in duplicates, interpolated from the CD86 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).](https://storage.huabio.cn/huabio/productImg/HA723553_4.png?v=20260401002340)
Interpolated concentrations of spiked CD86 in cell culture media samples.
Capture: HA723552, Mouse CD86 Rabbit mAb [PSH13-63]
Detector: HA723553, Mouse CD86 Rabbit mAb [PSH13-64]
The concentrations of CD86 were measured in duplicates, interpolated from the CD86 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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