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TBC1D1 Recombinant Rabbit Monoclonal Antibody [PSH13-71]

Usd: 385

Specification

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Catalog# HA723560

TBC1D1 Recombinant Rabbit Monoclonal Antibody [PSH13-71]

Application

  • WB

  • IHC-P

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

TBC1D1 Recombinant Rabbit Monoclonal Antibody [PSH13-71]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human TBC1D1 aa 1-430.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IP

Molecular Weight

Predicted band size: 133 kDa

Positive Control

LNCaP cell lysate, Jurkat cell lysate, C2C12 cell lysate, PC-12 cell lysate, mouse kidney tissue, mouse testis tissue.

Conjugation

unconjugated

Clone Number

PSH13-71

Reactivity Data

WB IHC-P IP
Human
Tested Tested
Not Recommended
Tested Tested
Mouse
Tested Tested
Tested Tested
Rat
Tested Tested
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:10,000-1:50,000

  • IHC-P

  • 1:200

  • IP

  • 1-2μg/sample

Target

Function

TBC1 domain family member 1 is a protein that in humans is encoded by the TBC1D1 gene. TBC1D1 is the founding member of a family of proteins sharing a 180- to 200-amino acid TBC domain presumed to have a role in regulating cell growth and cell differentiation. These proteins share significant homology with TRE2 (USP6), yeast Bub2, and CDC16.

Background References

1. Song D et al. Novel prognostic biomarker TBC1D1 is associated with immunotherapy resistance in gliomas. Front Immunol. 2024 Mar

2. Wang Q et al. TBC1D1 is an energy-responsive polarization regulator of macrophages via governing ROS production in obesity. Sci China Life Sci. 2024 Sep

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q86TI0 Human

SwissProt: Q60949 Mouse

Entrez Gene: 360937 Rat

Synonyms

KIAA1108 antibody

TBC 1 antibody

TBC antibody

TBC1 (tre 2/USP6, BUB2, cdc16) domain family, member 1 antibody

TBC1 antibody

TBC1 domain family member 1 antibody

Tbc1d1 antibody

TBCD1_HUMAN antibody

Images

  • Western blot analysis of TBC1D1 on different lysates with Rabbit anti-TBC1D1 antibody (<a href="/products/HA723560" style="font-weight: bold;text-decoration: underline;">HA723560</a>) at 1/10,000 dilution.<br /><br />Lane 1: LNCaP cell lysate (20 µg/Lane)<br />Lane 2: Jurkat cell lysate (20 µg/Lane)<br />Lane 3: C2C12 cell lysate (20 µg/Lane)<br />Lane 4: PC-12 cell lysate (20 µg/Lane)<br /><br />Predicted band size: 133 kDa<br />Observed band size: 150 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723560" style="font-weight: bold;text-decoration: underline;">HA723560</a>) at 1/10,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TBC1D1 on different lysates with Rabbit anti-TBC1D1 antibody (HA723560) at 1/10,000 dilution.

    Lane 1: LNCaP cell lysate (20 µg/Lane)
    Lane 2: Jurkat cell lysate (20 µg/Lane)
    Lane 3: C2C12 cell lysate (20 µg/Lane)
    Lane 4: PC-12 cell lysate (20 µg/Lane)

    Predicted band size: 133 kDa
    Observed band size: 150 kDa

    Exposure time: 25 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723560) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-TBC1D1 antibody (<a href="/products/HA723560" style="font-weight: bold;text-decoration: underline;">HA723560</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723560" style="font-weight: bold;text-decoration: underline;">HA723560</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-TBC1D1 antibody (HA723560) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723560) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-TBC1D1 antibody (<a href="/products/HA723560" style="font-weight: bold;text-decoration: underline;">HA723560</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723560" style="font-weight: bold;text-decoration: underline;">HA723560</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-TBC1D1 antibody (HA723560) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723560) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • TBC1D1 was immunoprecipitated from 0.2 mg Jurkat cell lysate with <a href="/products/HA723560" style="font-weight: bold;text-decoration: underline;">HA723560</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA723560" style="font-weight: bold;text-decoration: underline;">HA723560</a> at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: Jurkat cell lysate (input)<br />Lane 2: <a href="/products/HA723560" style="font-weight: bold;text-decoration: underline;">HA723560</a> IP in Jurkat cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA723560" style="font-weight: bold;text-decoration: underline;">HA723560</a> in Jurkat cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 3 minutes; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    TBC1D1 was immunoprecipitated from 0.2 mg Jurkat cell lysate with HA723560 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723560 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: Jurkat cell lysate (input)
    Lane 2: HA723560 IP in Jurkat cell lysate
    Lane 3: Rabbit IgG instead of HA723560 in Jurkat cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 3 minutes; ECL: K1801

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