Human Lipoprotein A Recombinant Rabbit Monoclonal Antibody [PSH13-81] - BSA and Azide free (Capture)
Catalog# HA723570
Human Lipoprotein A Recombinant Rabbit Monoclonal Antibody [PSH13-81] - BSA and Azide free (Capture)
-
ELISA(Cap)
-
Human
-
Cynomolgus monkey
-
15.6-2,000 pg/mL
-
6.08
-
unconjugated
Overview
Product Name
Human Lipoprotein A Recombinant Rabbit Monoclonal Antibody [PSH13-81] - BSA and Azide free (Capture)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human LPA aa 20-1,033 (HA210979).
Species Reactivity
Human, Cynomolgus monkey
Validated Applications
ELISA(Cap)
Positive Control
Recombinant Human Lipoprotein A protein (HA210979).
Conjugation
unconjugated
Clone Number
PSH13-81
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Cap)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH13-82] to Human Lipoprotein A antibody (Detector) (HA723571) or Rabbit monoclonal [PSH13-83] to Human Lipoprotein A antibody (Detector) (HA723573) and recombinant Human Lipoprotein A protein (HA210979) as the standard. The reference range value is 15.6-2,000 pg/mL.
Target
Function
Lipoprotein(a) is a low-density lipoprotein variant containing a protein called apolipoprotein(a). Genetic and epidemiological studies have identified lipoprotein(a) as a risk factor for atherosclerosis and related diseases, such as coronary heart disease and stroke. Lp(a) is assembled at the hepatocyte cell membrane surface, which is similar to typical LDL particles. However, there are other possible locations of assembly. The particles mainly exist in plasma. Lp(a) contributes to the process of atherogenesis. The structure of apolipoprotein(a) is similar to plasminogen and tPA (tissue plasminogen activator) and it competes with plasminogen for its binding site, leading to reduced fibrinolysis. Also, because Lp(a) stimulates secretion of PAI-1, it leads to thrombogenesis. It also may enhance coagulation by inhibiting the function of tissue factor pathway inhibitor.
Background References
1. Lampsas S et al. Lipoprotein(a) in Atherosclerotic Diseases: From Pathophysiology to Diagnosis and Treatment. Molecules. 2023 Jan
2. Nordestgaard BG et al. Lipoprotein(a) and cardiovascular disease. Lancet. 2024 Sep
Subcellular Location
Extracellular region, plasma lipoprotein particle.
UNIPROT
Synonyms
AK38 antibody
Antiangiogenic AK38 protein antibody
apo(a) antibody
APOA antibody
apolipoprotein(a) antibody
LP antibody
lp(a) antibody
LPA antibody
Images
-
Sandwich ELISA analysis of human Lipoprotein A matched pair antibodies
Capture: HA723570, Human Lipoprotein A Rabbit mAb [PSH13-81]
Detector: HA723571, Human Lipoprotein A Rabbit mAb [PSH13-82]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723570) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human Lipoprotein A protein (HA210979) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723571, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
Sandwich ELISA analysis of human Lipoprotein A matched pair antibodies
Capture: HA723570, Human Lipoprotein A Rabbit mAb [PSH13-81]
Detector: HA723573, Human Lipoprotein A Rabbit mAb [PSH13-83]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723570) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human Lipoprotein A protein (HA210979) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723573, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
Interpolated concentrations of native Lipoprotein A in human urine samples.
Capture: HA723570, Human Lipoprotein A Rabbit mAb [PSH13-81]
Detector: HA723571, Human Lipoprotein A Rabbit mAb [PSH13-82]
The concentrations of Lipoprotein A were measured in duplicates, interpolated from the Lipoprotein A standard curve and corrected for sample dilution. Undiluted samples are human urine 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lipoprotein A concentration was determined to be 5,193 pg/ml in human urine. -
Interpolated concentrations of native Lipoprotein A in human urine samples.
Capture: HA723570, Human Lipoprotein A Rabbit mAb [PSH13-81]
Detector: HA723573, Human Lipoprotein A Rabbit mAb [PSH13-83]
The concentrations of Lipoprotein A were measured in duplicates, interpolated from the Lipoprotein A standard curve and corrected for sample dilution. Undiluted samples are human urine 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lipoprotein A concentration was determined to be 4,023 pg/ml in human urine. -
Interpolated concentrations of native Lipoprotein A in human serum samples.
Capture: HA723570, Human Lipoprotein A Rabbit mAb [PSH13-81]
Detector: HA723571, Human Lipoprotein A Rabbit mAb [PSH13-82]
The concentrations of Lipoprotein A were measured in duplicates, interpolated from the Lipoprotein A standard curve and corrected for sample dilution. Undiluted samples are human serum 0.033%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lipoprotein A concentration was determined to be 4.48 µg/ml in human serum. -
Interpolated concentrations of native Lipoprotein A in human serum samples.
Capture: HA723570, Human Lipoprotein A Rabbit mAb [PSH13-81]
Detector: HA723573, Human Lipoprotein A Rabbit mAb [PSH13-83]
The concentrations of Lipoprotein A were measured in duplicates, interpolated from the Lipoprotein A standard curve and corrected for sample dilution. Undiluted samples are human serum 0.004%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lipoprotein A concentration was determined to be 16.01 µg/ml in human serum. -
Interpolated concentrations of spiked Lipoprotein A in human cell culture media samples.
Capture: HA723570, Human Lipoprotein A Rabbit mAb [PSH13-81]
Detector: HA723571, Human Lipoprotein A Rabbit mAb [PSH13-82]
The concentrations of Lipoprotein A were measured in duplicates, interpolated from the Lipoprotein A standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). -
Interpolated concentrations of spiked Lipoprotein A in human cell culture media samples.
Capture: HA723570, Human Lipoprotein A Rabbit mAb [PSH13-81]
Detector: HA723573, Human Lipoprotein A Rabbit mAb [PSH13-83]
The concentrations of Lipoprotein A were measured in duplicates, interpolated from the Lipoprotein A standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"