CHCHD2 Recombinant Rabbit Monoclonal Antibody [PSH13-87]
Catalog# HA723580
CHCHD2 Recombinant Rabbit Monoclonal Antibody [PSH13-87]
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WB
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IF-Cell
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FC
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IP
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Human
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Mouse
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Rat
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HA751489
不含抗保成分
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unconjugated
Overview
Product Name
CHCHD2 Recombinant Rabbit Monoclonal Antibody [PSH13-87]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human CHCHD2 aa 1-151.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, FC, IP
Molecular Weight
Predicted band size: 16 kDa
Positive Control
A431 cell lysate, HeLa cell lysate, HepG2 cell lysate, K-562 cell lysate, Jurkat cell lysate, HEK-293 cell lysate, LNCaP cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, Mouse kidney tissue lysate, A431.
Conjugation
unconjugated
Clone Number
PSH13-87
Reactivity Data
| WB | IF-Cell | FC | IP | |
|---|---|---|---|---|
| Human |
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| Mouse |
|
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| Rat |
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Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IF-Cell
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1:100
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FC
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1:1,000
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IP
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1-2μg/sample
Target
Function
Coiled-coil-helix-coiled-coil-helix domain containing 2 is a protein that in humans is encoded by the CHCHD2 gene. The protein encoded by this gene belongs to a class of eukaryotic CX(9)C proteins characterized by four cysteine residues spaced ten amino acids apart from one another. These residues form disulfide linkages that define a CHCH fold. In response to stress, the protein translocates from the mitochondrial intermembrane space to the nucleus where it binds to a highly conserved 13 nucleotide oxygen responsive element in the promoter of cytochrome oxidase 4I2, a subunit of the terminal enzyme of the electron transport chain. In concert with recombination signal sequence-binding protein J, binding of this protein activates the oxygen responsive element at four percent oxygen. In addition, it has been shown that this protein is a negative regulator of mitochondria-mediated apoptosis. In response to apoptotic stimuli, mitochondrial levels of this protein decrease, allowing BCL2-associated X protein to oligomerize and activate the Caspase Cascade. Pseudogenes of this gene are found on multiple chromosomes. Alternative splicing results in multiple transcript variants.
Background References
1. Kee TR et al. Mitochondrial CHCHD2: Disease-Associated Mutations, Physiological Functions, and Current Animal Models. Front Aging Neurosci. 2021 Apr
2. Leventoux N et al. Aberrant CHCHD2-associated mitochondriopathy in Kii ALS/PDC astrocytes. Acta Neuropathol. 2024 May
Subcellular Location
Nucleus, Mitochondrion, Mitochondrion intermembrane space.
Synonyms
16.7kD protein antibody
Aging Associated Gene 10 Protein antibody
Aging-associated gene 10 protein antibody
C7orf17 antibody
CHCH2_HUMAN antibody
CHCHD 2 antibody
CHCHD2 antibody
Coiled Coil Helix Coiled Coil Helix Domain Containing 2 antibody
Coiled coil helix coiled coil helix domain containing protein 2, mitochondrial antibody
Coiled-coil-helix-coiled-coil-helix domain-containing protein 2, mitochondrial antibody
ExpandImages
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Western blot analysis of CHCHD2 on different lysates with Rabbit anti-CHCHD2 antibody (HA723580) at 1/5,000 dilution.
Lane 1: A431 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: HepG2 cell lysate (20 µg/Lane)
Lane 4: K-562 cell lysate (20 µg/Lane)
Lane 5: Jurkat cell lysate (20 µg/Lane)
Lane 6: HEK-293 cell lysate (20 µg/Lane)
Lane 7: LNCaP cell lysate (20 µg/Lane)
Lane 8: Neuro-2a cell lysate (20 µg/Lane)
Lane 9: PC-12 cell lysate (20 µg/Lane)
Lane 10: Mouse kidney tissue lysate (40 µg/Lane)
Predicted band size: 16 kDa
Observed band size: 17 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723580) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of A431 cells labeling CHCHD2 with Rabbit anti-CHCHD2 antibody (HA723580) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CHCHD2 antibody (HA723580) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI. -
Flow cytometric analysis of A431 cells labeling CHCHD2.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA723580, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
CHCHD2 was immunoprecipitated from 0.2 mg A431 cell lysate with HA723580 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723580 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: A431 cell lysate (input)
Lane 2: HA723580 IP in A431 cell lysate
Lane 3: Rabbit IgG instead of HA723580 in A431 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 6 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"