Bid Recombinant Rabbit Monoclonal Antibody [PSH14-03]
Catalog# HA723593
Bid Recombinant Rabbit Monoclonal Antibody [PSH14-03]
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WB
-
IP
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Human
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Mouse
-
unconjugated
Overview
Product Name
Bid Recombinant Rabbit Monoclonal Antibody [PSH14-03]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within mouse Bid aa 51-195.
Species Reactivity
Human, Mouse
Validated Applications
WB, IP
Target Molecular Weight
Predicted band size: 22 kDa
Positive Control
Jurkat cell lysate, HT-29 cell lysate, A549 cell lysate, RAW264.7 cell lysate.
Conjugation
unconjugated
Clone Number
PSH14-03
Reactivity Data
| WB | IP | |
|---|---|---|
| Human |
|
|
| Mouse |
|
|
| Green Monkey |
|
|
| Rat |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:5,000
-
IP
-
1-2μg/sample
Target
Function
The BH3 interacting-domain death agonist, or BID, gene is a pro-apoptotic member of the Bcl-2 protein family. Bcl-2 family members share one or more of the four characteristic domains of homology entitled the Bcl-2 homology (BH) domains (named BH1, BH2, BH3 and BH4), and can form hetero- or homodimers. Bcl-2 proteins act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities.
Background References
1. Wyżewski Z et al. Bid Protein: A Participant in the Apoptotic Network with Roles in Viral Infections. Int J Mol Sci. 2025 Mar
2. Bertran-Alamillo J et al. BID expression determines the apoptotic fate of cancer cells after abrogation of the spindle assembly checkpoint by AURKB or TTK inhibitors. Mol Cancer. 2023 Jul
Subcellular Location
Cytoplasm, Mitochondrion membrane, Mitochondrion outer membrane.
Synonyms
Apoptic death agonist antibody
Apoptotic death agonist BID antibody
BH3 interacting domain death agonist antibody
BH3 interacting domain death agonist p11 antibody
BH3 interacting domain death agonist p13 antibody
BH3 interacting domain death agonist p15 antibody
BH3-interacting domain death agonist p11 antibody
BID antibody
BID isoform ES(1b) antibody
BID isoform L(2) antibody
ExpandImages
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Western blot analysis of Bid on different lysates with Rabbit anti-Bid antibody (HA723593) at 1/5,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: HT-29 cell lysate
Lane 3: A549 cell lysate
Lane 4: RAW264.7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723593) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Bid was immunoprecipitated from 0.2 mg RAW264.7 cell lysate with HA723593 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723593 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: RAW264.7 cell lysate (input)
Lane 2: HA723593 IP in RAW264.7 cell lysate
Lane 3: Rabbit IgG instead of HA723593 in RAW264.7 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 46 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Bid Recombinant Rabbit Monoclonal Antibody [PSH14-03] - BSA and Azide free
Application: WB,IP
Reactivity: Human,Mouse
Conjugate: unconjugated
