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PRAK / MK5 Recombinant Rabbit Monoclonal Antibody [PSH14-06]

Usd: 385

Specification

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Catalog# HA723596

PRAK / MK5 Recombinant Rabbit Monoclonal Antibody [PSH14-06]

Application

  • WB

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

PRAK / MK5 Recombinant Rabbit Monoclonal Antibody [PSH14-06]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human PRAK / MK5 aa 251-473.

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB, IP

Molecular Weight

Predicted band size: 54 kDa

Positive Control

HeLa cell lysate, A431 cell lysate, A549 cell lysate, MDA-MB-231 cell lysate, Jurkat cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, MEF cell lysate, C6 cell lysate, PC-12 cell lysate.

Conjugation

unconjugated

Clone Number

PSH14-06

Reactivity Data

WB IP
Human
Tested Tested
Tested Tested
Mouse
Tested Tested
Rat
Tested Tested
Green Monkey
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IP

  • 1-2μg/sample

Target

Function

MAP kinase-activated protein kinase 5 is an enzyme that in humans is encoded by the MAPKAPK5 gene. The protein encoded by this gene is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases, including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately-spliced transcript variants of this gene encoding distinct isoforms have been reported. A link between Alzheimer's disease and reduced levels of MAPKAPK5 has been proposed. Clinical trials may confirm if this is the case.

Background References

1. Wang Y et al. The TTYH3/MK5 Positive Feedback Loop regulates Tumor Progression via GSK3-β/β-catenin signaling in HCC. Int J Biol Sci. 2022 Jun

2. Soulez M et al. ERK3-MK5 signaling regulates myogenic differentiation and muscle regeneration by promoting FoxO3 degradation. J Cell Physiol. 2022 Apr

Subcellular Location

Cytoplasm, Nucleus.

UNIPROT

SwissProt: Q8IW41 Human

SwissProt: O54992 Mouse

Entrez Gene: 498183 Rat

Synonyms

MAP kinase-activated protein kinase 5 antibody

MAPK-activated protein kinase 5 antibody

MAPK5_HUMAN antibody

MAPKAP kinase 5 antibody

MAPKAPK-5 antibody

MAPKAPK5 antibody

Mitogen activated protein kinase activated protein kinase 5 antibody

p38-regulated/activated protein kinase antibody

PRAK antibody

Images

  • Western blot analysis of PRAK / MK5 on different lysates with Rabbit anti-PRAK / MK5 antibody (<a href="/products/HA723596" style="font-weight: bold;text-decoration: underline;">HA723596</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: A431 cell lysate<br />Lane 3: A549 cell lysate<br />Lane 4: MDA-MB-231 cell lysate<br />Lane 5: Jurkat cell lysate<br />Lane 6: COS-1 cell lysate<br />Lane 7: NIH/3T3 cell lysate<br />Lane 8: MEF cell lysate<br />Lane 9: C6 cell lysate<br />Lane 10: PC-12 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 54 kDa<br />Observed band size: 52/66 kDa<br /><br />Exposure time: 59 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723596" style="font-weight: bold;text-decoration: underline;">HA723596</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PRAK / MK5 on different lysates with Rabbit anti-PRAK / MK5 antibody (HA723596) at 1/5,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: A431 cell lysate
    Lane 3: A549 cell lysate
    Lane 4: MDA-MB-231 cell lysate
    Lane 5: Jurkat cell lysate
    Lane 6: COS-1 cell lysate
    Lane 7: NIH/3T3 cell lysate
    Lane 8: MEF cell lysate
    Lane 9: C6 cell lysate
    Lane 10: PC-12 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 54 kDa
    Observed band size: 52/66 kDa

    Exposure time: 59 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723596) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • PRAK / MK5 was immunoprecipitated from 0.2 mg HeLa cell lysate with <a href="/products/HA723596" style="font-weight: bold;text-decoration: underline;">HA723596</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA723596" style="font-weight: bold;text-decoration: underline;">HA723596</a> at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: HeLa cell lysate (input)<br />Lane 2: <a href="/products/HA723596" style="font-weight: bold;text-decoration: underline;">HA723596</a> IP in HeLa cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA723596" style="font-weight: bold;text-decoration: underline;">HA723596</a> in HeLa cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 30 seconds; ECL: <a href="/products/K1802" style="font-weight: bold;text-decoration: underline;">K1802</a>

    PRAK / MK5 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA723596 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723596 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: HeLa cell lysate (input)
    Lane 2: HA723596 IP in HeLa cell lysate
    Lane 3: Rabbit IgG instead of HA723596 in HeLa cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 30 seconds; ECL: K1802

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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metafield image

PRAK / MK5 Recombinant Rabbit Monoclonal Antibody [PSH14-06] - BSA and Azide free

Application: WB,IP

Reactivity: Human,Mouse,Rat,Green monkey

Conjugate: unconjugated