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NDUFA9 Recombinant Rabbit Monoclonal Antibody [PSH14-08]

Usd: 385

Specification

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Catalog# HA723598

NDUFA9 Recombinant Rabbit Monoclonal Antibody [PSH14-08]

Application

  • WB

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

NDUFA9 Recombinant Rabbit Monoclonal Antibody [PSH14-08]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human NDUFA9 aa 1-230.

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB, IP

Molecular Weight

Predicted band size: 43 kDa

Positive Control

HepG2 cell lysate, HeLa cell lysate, U-2 OS cell lysate, Caco-2 cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, Neuro-2a cell lysate, C6 cell lysate, L6 cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate, Mouse skeletal muscle tissue lysate, Rat skeletal muscle tissue lysate.

Conjugation

unconjugated

Clone Number

PSH14-08

Reactivity Data

WB IP
Human
Tested Tested
Tested Tested
Mouse
Tested Tested
Rat
Tested Tested
Green Monkey
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IP

  • 1-2μg/sample

Target

Function

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9 is an enzyme that in humans is encoded by the NDUFA9 gene. The NDUFA9 protein is a subunit of NADH:ubiquinone oxidoreductase (Complex I of the electron transport chain), which is located in the mitochondrial inner membrane and is the largest of the five complexes of the electron transport chain. Mutations in NADH dehydrogenase (ubiquinone), also known as Complex I, frequently lead to complex neurodegenerative diseases such as Leigh's syndrome. In the case of NDUFA9, a mutation to the MT-ND3 gene might interrupt their interaction and formation of subcomplexes, compromising Complex I function and leading to disease.

Background References

1. Liu Y et al. NDUFA9 and its crotonylation modification promote browning of white adipocytes by activating mitochondrial function in mice. Int J Biochem Cell Biol. 2024 Jun

2. Nesti C et al. Additive effect of DNAJC30 and NDUFA9 mutations causing Leigh syndrome. J Neurol. 2023 Jun

Subcellular Location

Mitochondrion matrix.

UNIPROT

SwissProt: Q16795 Human

SwissProt: Q9DC69 Mouse

SwissProt: Q5BK63 Rat

Synonyms

CI-39kD antibody

Complex I subunit NDUFA9 antibody

Complex I-39kD antibody

mitochondrial antibody

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9 antibody

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9 mitochondrial antibody

NADH Ubiquinone Oxidoreductase 1 alpha subcomplex 9 antibody

NADH-ubiquinone oxidoreductase 39 kDa subunit antibody

NDUA9_HUMAN antibody

NDUFA9 antibody

Images

  • Western blot analysis of NDUFA9 on different lysates with Rabbit anti-NDUFA9 antibody (<a href="/products/HA723598" style="font-weight: bold;text-decoration: underline;">HA723598</a>) at 1/5,000 dilution.<br /><br />Lane 1: HepG2 cell lysate<br />Lane 2: HeLa cell lysate<br />Lane 3: U-2 OS cell lysate<br />Lane 4: Caco-2 cell lysate<br />Lane 5: COS-1 cell lysate<br />Lane 6: NIH/3T3 cell lysate<br />Lane 7: Neuro-2a cell lysate<br />Lane 8: C6 cell lysate<br />Lane 9: L6 cell lysate<br />Lane 10: Mouse liver tissue lysate<br />Lane 11: Rat liver tissue lysate<br />Lane 12: Mouse skeletal muscle tissue lysate<br />Lane 13: Rat skeletal muscle tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 43 kDa<br />Observed band size: 36 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723598" style="font-weight: bold;text-decoration: underline;">HA723598</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of NDUFA9 on different lysates with Rabbit anti-NDUFA9 antibody (HA723598) at 1/5,000 dilution.

    Lane 1: HepG2 cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: U-2 OS cell lysate
    Lane 4: Caco-2 cell lysate
    Lane 5: COS-1 cell lysate
    Lane 6: NIH/3T3 cell lysate
    Lane 7: Neuro-2a cell lysate
    Lane 8: C6 cell lysate
    Lane 9: L6 cell lysate
    Lane 10: Mouse liver tissue lysate
    Lane 11: Rat liver tissue lysate
    Lane 12: Mouse skeletal muscle tissue lysate
    Lane 13: Rat skeletal muscle tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 43 kDa
    Observed band size: 36 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723598) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • NDUFA9 was immunoprecipitated from 0.2 mg HepG2 cell lysate with <a href="/products/HA723598" style="font-weight: bold;text-decoration: underline;">HA723598</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA723598" style="font-weight: bold;text-decoration: underline;">HA723598</a> at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: HepG2 cell lysate (input)<br />Lane 2: <a href="/products/HA723598" style="font-weight: bold;text-decoration: underline;">HA723598</a> IP in HepG2 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA723598" style="font-weight: bold;text-decoration: underline;">HA723598</a> in HepG2 cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 15 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    NDUFA9 was immunoprecipitated from 0.2 mg HepG2 cell lysate with HA723598 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723598 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: HepG2 cell lysate (input)
    Lane 2: HA723598 IP in HepG2 cell lysate
    Lane 3: Rabbit IgG instead of HA723598 in HepG2 cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 15 seconds; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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