non-muscle Myosin IIA Recombinant Rabbit Monoclonal Antibody [PSH14-10]
Catalog# HA723600
non-muscle Myosin IIA Recombinant Rabbit Monoclonal Antibody [PSH14-10]
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WB
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IF-Cell
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IHC-P
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FC
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IP
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Human
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Mouse
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Rat
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HA751505
不含抗保成分
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unconjugated
Overview
Product Name
non-muscle Myosin IIA Recombinant Rabbit Monoclonal Antibody [PSH14-10]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human MYH9 aa 1,151-1,430.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P, FC, IP
Molecular Weight
Predicted band size: 227 kDa
Positive Control
HeLa cell lysate, HUVEC cell lysate, A431 cell lysate, HT-29 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, PC-12 cell lysate, A549 cell lysate, Mouse kidney tissue lysate, Mouse lung tissue lysate, Mouse liver tissue lysate, Rat kidney tissue lysate, Rat lung tissue lysate, Rat liver tissue lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue, rat liver tissue, HeLa, NIH/3T3.
Conjugation
unconjugated
Clone Number
PSH14-10
Reactivity Data
| WB | IF-Cell | IHC-P | FC | IP | |
|---|---|---|---|---|---|
| Human |
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| Mouse |
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| Rat |
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Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IF-Cell
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1:100
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IHC-P
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1:1,000
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FC
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1:1,000
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IP
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1-2μg/sample
Target
Function
Myosin-9 also known as myosin, heavy chain 9, non-muscle or non-muscle myosin heavy chain IIa (NMMHC-IIA) is a protein which in humans is encoded by the MYH9 gene. Non-muscle myosin IIA (NM IIA) is expressed in most cells and tissues where it participates in a variety of processes requiring contractile force, such as cytokinesis, cell migration, polarization and adhesion, maintenance of cell shape, and signal transduction. Myosin IIs are motor proteins that are part of a superfamily composed of more than 30 classes. Class II myosins include muscle and non-muscle myosins that are organized as hexameric molecules consisting of two heavy chains (230 kDa), two regulatory light chains (20 KDa) controlling the myosin activity, and two essential light chains (17 kDa), which stabilize the heavy chain structure.
Background References
1. Liu Q et al. MYH9: A key protein involved in tumor progression and virus-related diseases. Biomed Pharmacother. 2024 Feb
2. Lee S et al. Identification of MYH9 as a key regulator for synoviocyte migration and invasion through secretome profiling. Ann Rheum Dis. 2023 Aug
Subcellular Location
Cytoplasm, cytoskeleton, cell cortex, Cytoplasmic vesicle, secretory vesicle, Cortical granule, Cell membrane.
Synonyms
BDPLT 6 antibody
BDPLT6 antibody
Cellular myosin heavy chain antibody
Cellular myosin heavy chain type A antibody
DFNA 17 antibody
DFNA17 antibody
EPSTS antibody
FTNS antibody
MGC104539 antibody
MHA antibody
ExpandImages
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Western blot analysis of non-muscle Myosin IIA on different lysates with Rabbit anti-non-muscle Myosin IIA antibody (HA723600) at 1/5,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HUVEC cell lysate (20 µg/Lane)
Lane 3: A431 cell lysate (20 µg/Lane)
Lane 4: HT-29 cell lysate (20 µg/Lane)
Lane 5: NIH/3T3 cell lysate (20 µg/Lane)
Lane 6: C2C12 cell lysate (20 µg/Lane)
Lane 7: PC-12 cell lysate (20 µg/Lane)
Lane 8: A549 cell lysate (20 µg/Lane)
Lane 9: Mouse kidney tissue lysate (40 µg/Lane)
Lane 10: Mouse lung tissue lysate (40 µg/Lane)
Lane 11: Mouse liver tissue lysate (40 µg/Lane)
Lane 12: Rat kidney tissue lysate (40 µg/Lane)
Lane 13: Rat lung tissue lysate (40 µg/Lane)
Lane 14: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 227 kDa
Observed band size: 227 kDa
Exposure time: 1 minute 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723600) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-non-muscle Myosin IIA antibody (HA723600) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723600) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-non-muscle Myosin IIA antibody (HA723600) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723600) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-non-muscle Myosin IIA antibody (HA723600) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723600) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-non-muscle Myosin IIA antibody (HA723600) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723600) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunocytochemistry analysis of HeLa cells labeling non-muscle Myosin IIA with Rabbit anti-non-muscle Myosin IIA antibody (HA723600) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-non-muscle Myosin IIA antibody (HA723600) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of NIH/3T3 cells labeling non-muscle Myosin IIA with Rabbit anti-non-muscle Myosin IIA antibody (HA723600) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-non-muscle Myosin IIA antibody (HA723600) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of HeLa cells labeling non-muscle Myosin IIA.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA723600, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Flow cytometric analysis of NIH/3T3 cells labeling non-muscle Myosin IIA.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA723600, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
non-muscle Myosin IIA was immunoprecipitated from 0.2 mg HeLa cell lysate with HA723600 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723600 at 1/50,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: HA723600 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of HA723600 in HeLa cell lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 3 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"