TGF beta Receptor I Recombinant Rabbit Monoclonal Antibody [PSH14-14]
Catalog# HA723604
TGF beta Receptor I Recombinant Rabbit Monoclonal Antibody [PSH14-14]
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WB
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IP
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Human
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Mouse
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Rat
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HA751509
不含抗保成分
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unconjugated
Overview
Product Name
TGF beta Receptor I Recombinant Rabbit Monoclonal Antibody [PSH14-14]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human TGF beta Receptor I aa 34-503.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IP
Molecular Weight
Predicted band size: 56 kDa
Positive Control
THP-1 cell lysate, MCF7 cell lysate, SH-SY5Y cell lysate, Neuro-2a cell lysate, RAW264.7 cell lysate, C6 cell lysate.
Conjugation
unconjugated
Clone Number
PSH14-14
Reactivity Data
| WB | IP | |
|---|---|---|
| Human |
|
|
| Mouse |
|
|
| Rat |
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IP
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1-2μg/sample
Target
Function
Transforming growth factor beta receptor I (activin A receptor type II-like kinase, 53kDa) is a membrane-bound TGF beta receptor protein of the TGF-beta receptor family for the TGF beta superfamily of signaling ligands. TGFBR1 is its human gene. The protein encoded by this gene forms a heteromeric complex with type II TGF-β receptors when bound to TGF-β, transducing the TGF-β signal from the cell surface to the cytoplasm. The encoded protein is a serine/threonine protein kinase. Mutations in this gene have been associated with Loeys–Dietz aortic aneurysm syndrome (LDS, LDAS).
Background References
1. Wang J et al. Nestin promotes pulmonary fibrosis via facilitating recycling of TGF-beta receptor I. Eur Respir J. 2022 May
2. Ghorbaninejad M et al. TGF-beta receptor I inhibitor may restrict the induction of EMT in inflamed intestinal epithelial cells. Exp Biol Med (Maywood). 2023 Apr
Subcellular Location
Cell membrane, Cell junction, tight junction, Cell surface, Membrane raft.
Synonyms
AAT 5 antibody
AAT5 antibody
Activin A receptor type II like kinase 53kDa antibody
Activin A receptor type II like kinase antibody
Activin A receptor type II like kinase, 53kD antibody
Activin A receptor type II like protein kinase of 53kD antibody
activin A receptor type II-like kinase, 53kDa antibody
activin A receptor type II-like protein kinase of 53kD antibody
Activin receptor like kinase 5 antibody
Activin receptor-like kinase 5 antibody
ExpandImages
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Western blot analysis of TGF beta Receptor I on different lysates with Rabbit anti-TGF beta Receptor I antibody (HA723604) at 1/2,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: MCF7 cell lysate
Lane 3: SH-SY5Y cell lysate
Lane 4: Neuro-2a cell lysate
Lane 5: RAW264.7 cell lysate
Lane 6: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 56 kDa
Observed band size: 50/37 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723604) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
TGF beta Receptor I was immunoprecipitated from 0.2 mg THP-1 cell lysate with HA723604 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723604 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: THP-1 cell lysate (input)
Lane 2: HA723604 IP in THP-1 cell lysate
Lane 3: Rabbit IgG instead of HA723604 in THP-1 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 1 minute 7 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
TGF beta Receptor I Recombinant Rabbit Monoclonal Antibody [PSH14-14] - BSA and Azide free
Application: WB,IP
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
