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FLI1 Recombinant Rabbit Monoclonal Antibody [PSH14-17]

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Specification

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Catalog# HA723605

FLI1 Recombinant Rabbit Monoclonal Antibody [PSH14-17]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

FLI1 Recombinant Rabbit Monoclonal Antibody [PSH14-17]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human FLI1 aa 351-400 / 452.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 51 kDa

Positive Control

U-937 cell lysate, Jurkat cell lysate, human B cell lymphoma tissue, human lung tissue.

Conjugation

unconjugated

Clone Number

PSH14-17

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

Target

Function

Friend leukemia integration 1 transcription factor (FLI1), also known as transcription factor ERGB, is a protein that in humans is encoded by the FLI1 gene, which is a proto-oncogene. Fli-1 is a member of the ETS transcription factor family that was first identified in erythroleukemias induced by Friend Murine Leukemia Virus (F-MuLV). Fli-1 is activated through retroviral insertional mutagenesis in 90% of F-MuLV-induced erythroleukemias. The constitutive activation of fli-1 in erythroblasts leads to a dramatic shift in the Epo/Epo-R signal transduction pathway, blocking erythroid differentiation, activating the Ras pathway, and resulting in massive Epo-independent proliferation of erythroblasts. These results suggest that Fli-1 overexpression in erythroblasts alters their responsiveness to Epo and triggers abnormal proliferation by switching the signaling event(s) associated with terminal differentiation to proliferation.

Background References

1. Mikhailova EV et al. Fli1 and Tissue Fibrosis in Various Diseases. Int J Mol Sci. 2023 Jan

2. Chen E et al. FLI1 promotes IFN-gamma-induced kynurenine production to impair anti-tumor immunity. Nat Commun. 2024 May

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q01543 Human

Synonyms

ERGB transcription factor antibody

Ewing Sarcoma breakpoint region 2 antibody

EWSR 2 antibody

EWSR2 antibody

FLI 1 antibody

FLI 1 proto oncogene antibody

Fli-1 proto-oncogene, ETS transcription factor antibody

FLI1 antibody

FLI1 EWS fusion gene antibody

FLI1 proto oncogene antibody

Expand

Images

  • Western blot analysis of FLI1 on different lysates with Rabbit anti-FLI1 antibody (<a href="/products/HA723605" style="font-weight: bold;text-decoration: underline;">HA723605</a>) at 1/1,000 dilution.<br /><br />Lane 1: U-937 cell lysate<br />Lane 2: Jurkat cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 51 kDa<br />Observed band size: 51 kDa<br /><br />Exposure time: 2 minutes 6 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723605" style="font-weight: bold;text-decoration: underline;">HA723605</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of FLI1 on different lysates with Rabbit anti-FLI1 antibody (HA723605) at 1/1,000 dilution.

    Lane 1: U-937 cell lysate
    Lane 2: Jurkat cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 51 kDa
    Observed band size: 51 kDa

    Exposure time: 2 minutes 6 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723605) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human B cell lymphoma tissue with Rabbit anti-FLI1 antibody (<a href="/products/HA723605" style="font-weight: bold;text-decoration: underline;">HA723605</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723605" style="font-weight: bold;text-decoration: underline;">HA723605</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human B cell lymphoma tissue with Rabbit anti-FLI1 antibody (HA723605) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723605) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-FLI1 antibody (<a href="/products/HA723605" style="font-weight: bold;text-decoration: underline;">HA723605</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723605" style="font-weight: bold;text-decoration: underline;">HA723605</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-FLI1 antibody (HA723605) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723605) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"